LOCUS BBa_K2150026 4861 bp DNA linear 22-Dec-2024 DEFINITION pT7+pTet+RBS+tetX-GFP(fusion protein)+RBS+T7RNAP+DT ACCESSION BBa_K2150026 VERSION BBa_K2150026.1 FEATURES Location/Qualifiers promoter 1..20 /label=BBa_K2150031 misc_feature 29..47 /label=TetR 1 misc_feature 29..82 /label=BBa_R0040 promoter 48..53 /label=-35 misc_feature 54..72 /label=TetR 2 promoter 71..76 /label=-10 promoter 29..82 /label=BBa_R0040 misc_feature 95..98 /label=conserved RBS 91..102 /label=BBa_B0034 CDS 109..1279 /label=TetX misc_feature 1280..1323 /label=protein linker CDS 1324..2043 /label=GFP CDS 109..2043 /label=BBa_K2150013 misc_feature 2056..2059 /label=conserved RBS 2052..2063 /label=BBa_B0034 promoter 4567..4594 /label=putative E. coli promoter CDS 2070..4724 /label=BBa_I2032 stem_loop 4744..4787 /label=stem_loop misc_feature 4733..4812 /label=BBa_B0010 terminator 4733..4812 /label=BBa_B0010 stem_loop 4828..4847 /label=T7 TE misc_feature 4854..4854 /label=stop polyA_site 4848..4861 /label=polya misc_feature 4821..4861 /label=BBa_B0012 terminator 4821..4861 /label=BBa_B0012 terminator 4733..4861 /label=BBa_B0015 ORIGIN 1 taatacgact cactataggg tactagagtc cctatcagtg atagagattg acatccctat 61 cagtgataga gatactgagc actactagag aaagaggaga aatactagat gaaaaaaacc 121 atgcgtatcg acaccgacaa acagatgaac ctgctgtctg acaaaaacgt tgcgatcatc 181 ggtggtggtc cggttggtct gaccatggcg aaactgcttc agcagaacgg tatcgacgtt 241 tctgtttacg aacgtgacaa cgaccgtgaa gcgcgtatct tcggtggtac cctggacctg 301 cacaaaggtt ctggtcagga agcgatgaaa aaagcgggtc tgcttcagac ctactacgac 361 ctggcgctgc cgatgggtgt taacatcgcg gacaaaaaag gtaacatcct gtctaccaaa 421 aacgttaaac cggaaaaccg tttcgacaac ccggaaatca accgtaacga cctgcgtgcg 481 atcctgctga actctctgga aaacgacacc gttatctggg accgtaaact ggttatgctg 541 gaaccgggta aaaaaaaatg gaccctgacc ttcgaaaaca aaccgtctga aaccgcggac 601 ctggttatcc tggcgaacgg tggtatgtct aaagttcgta aattcgttac cgacaccgaa 661 gttgaagaaa ccggtacctt caacatccag gcggacatcc accagccgga aatcaactgc 721 ccgggtttct tccagctgtg caacggtaac cgtctgatgg cgtctcacca gggtaacctg 781 ctgttcgcga acccgaacaa caacggtgcg ctgcacttcg gtatctcttt caaaaccccg 841 gacgaatgga aaaaccagac ccaggttgac ttccagaacc gtaactctgt tgttgacttc 901 ctgctgaaag agttctctga ctgggacgaa cgttacaaag aactgatcca caccaccctg 961 tctttcgttg gtctggcgac ccgtatcttc ccgctggaaa aaccgtggaa atctaaacgt 1021 ccgctgccga tcaccatgat cggtgacgcg gcgcacctga tgccgccgtt cgcgggtcag 1081 ggtgttaact ctggtctggt tgacgcgctg atcctgtctg acaacctggc ggacggtaaa 1141 ttcaactcta tcgaagaagc ggttaaaaac tacgaacagc agatgttcat gtacggtaaa 1201 gaagcgcagg aagaatctac ccagaacgaa atcgaaatgt tcaaaccgga cttcaccttc 1261 cagcagctgc tgaacgttgg tggaggaggt tctggaggcg gtggaagtgg tggcggaggt 1321 agcatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 1381 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac 1441 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca 1501 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa 1561 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt 1621 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt 1681 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac 1741 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat 1801 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca 1861 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 1921 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc 1981 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa 2041 taatactaga gaaagaggag aaatactaga tgaacacgat taacatcgct aagaacgact 2101 tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc 2161 gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct 2221 tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca 2281 agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg 2341 aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc 2401 cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca 2461 atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct 2521 tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca 2581 acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc 2641 tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc 2701 atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc 2761 gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg 2821 ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt 2881 gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc 2941 gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg 3001 tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca 3061 acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg 3121 acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc 3181 ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc 3241 gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc 3301 ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa 3361 tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac 3421 caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg 3481 ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg 3541 cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct 3601 tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct 3661 cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc 3721 gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct 3781 acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg 3841 ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc 3901 tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta 3961 agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc 4021 tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga 4081 atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag 4141 ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca 4201 aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg 4261 atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt 4321 tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg 4381 cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc 4441 ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc 4501 acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa 4561 ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg 4621 accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga 4681 acctccgtga catcttagag tcggacttcg cgttcgcgta ataatactag agccaggcat 4741 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4801 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4861 a //