##gff-version 3
##sequence-region BBa_K2150026 1 4861
BBa_K2150026	.	CDS	2070	4724	.	+	0	ID=BBa_I2032;Name=BBa_I2032
BBa_K2150026	.	promoter	4567	4594	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
BBa_K2150026	.	ribosome_entry_site	91	102	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150026	.	conserved_region	95	98	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150026	.	terminator	4733	4861	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150026	.	terminator	4733	4812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150026	.	engineered_region	4733	4812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150026	.	stem_loop	4744	4787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150026	.	terminator	4821	4861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150026	.	stop_codon	4854	4854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150026	.	polyA_site	4848	4861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150026	.	stem_loop	4828	4847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150026	.	engineered_region	4821	4861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150026	.	promoter	1	20	.	+	0	ID=BBa_K2150031;Name=BBa_K2150031
BBa_K2150026	.	CDS	109	2043	.	+	0	ID=BBa_K2150013;Name=BBa_K2150013
BBa_K2150026	.	CDS	109	1279	.	+	0	ID=annotation2489537;Name=TetX;Parent=BBa_K2150013
BBa_K2150026	.	tag	1280	1323	.	+	0	ID=annotation2489538;Name=protein linker;Parent=BBa_K2150013
BBa_K2150026	.	CDS	1324	2043	.	+	0	ID=annotation2489539;Name=GFP;Parent=BBa_K2150013
BBa_K2150026	.	ribosome_entry_site	2052	2063	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150026	.	conserved_region	2056	2059	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150026	.	promoter	29	82	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2150026	.	engineered_region	29	82	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2150026	.	promoter	48	53	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2150026	.	promoter	71	76	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2150026	.	non_covalent_binding_site	29	47	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2150026	.	non_covalent_binding_site	54	72	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_K2150026
taatacgactcactatagggtactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgag
cactactagagaaagaggagaaatactagatgaaaaaaaccatgcgtatcgacaccgacaaacagatgaacctgctgtc
tgacaaaaacgttgcgatcatcggtggtggtccggttggtctgaccatggcgaaactgcttcagcagaacggtatcgac
gtttctgtttacgaacgtgacaacgaccgtgaagcgcgtatcttcggtggtaccctggacctgcacaaaggttctggtc
aggaagcgatgaaaaaagcgggtctgcttcagacctactacgacctggcgctgccgatgggtgttaacatcgcggacaa
aaaaggtaacatcctgtctaccaaaaacgttaaaccggaaaaccgtttcgacaacccggaaatcaaccgtaacgacctg
cgtgcgatcctgctgaactctctggaaaacgacaccgttatctgggaccgtaaactggttatgctggaaccgggtaaaa
aaaaatggaccctgaccttcgaaaacaaaccgtctgaaaccgcggacctggttatcctggcgaacggtggtatgtctaa
agttcgtaaattcgttaccgacaccgaagttgaagaaaccggtaccttcaacatccaggcggacatccaccagccggaa
atcaactgcccgggtttcttccagctgtgcaacggtaaccgtctgatggcgtctcaccagggtaacctgctgttcgcga
acccgaacaacaacggtgcgctgcacttcggtatctctttcaaaaccccggacgaatggaaaaaccagacccaggttga
cttccagaaccgtaactctgttgttgacttcctgctgaaagagttctctgactgggacgaacgttacaaagaactgatc
cacaccaccctgtctttcgttggtctggcgacccgtatcttcccgctggaaaaaccgtggaaatctaaacgtccgctgc
cgatcaccatgatcggtgacgcggcgcacctgatgccgccgttcgcgggtcagggtgttaactctggtctggttgacgc
gctgatcctgtctgacaacctggcggacggtaaattcaactctatcgaagaagcggttaaaaactacgaacagcagatg
ttcatgtacggtaaagaagcgcaggaagaatctacccagaacgaaatcgaaatgttcaaaccggacttcaccttccagc
agctgctgaacgttggtggaggaggttctggaggcggtggaagtggtggcggaggtagcatgcgtaaaggagaagaact
tttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggt
gaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacac
ttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagag
tgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtc
aagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggac
acaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaactt
caaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggc
cctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccaca
tggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaa
gaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttca
acactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtga
agcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatc
actaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccga
cagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaac
cagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgt
atccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaaga
aagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataa
ggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaa
aatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcag
gtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtgg
ctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtt
tacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcg
ccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaacc
ggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggct
cgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcc
cttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggact
gcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggt
gtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctc
cactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtaca
gcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcg
atgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttg
ctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaa
cactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgc
agtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagata
ccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagct
gatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctg
gctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtt
tccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcc
taccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagc
caagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacg
actccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtc
ttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcactt
ccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata