LOCUS BBa_K2150027 5752 bp DNA linear 01-Jun-2024 DEFINITION pCon+RBS+tetR+DT+pT7+pTet+RBS+tetX-GFP(fusion protein)+RBS+T7RNAP+D T ACCESSION BBa_K2150027 VERSION BBa_K2150027.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 promoter 892..911 /label=BBa_K2150031 misc_feature 920..938 /label=TetR 1 misc_feature 920..973 /label=BBa_R0040 promoter 939..944 /label=-35 misc_feature 945..963 /label=TetR 2 promoter 962..967 /label=-10 promoter 920..973 /label=BBa_R0040 misc_feature 986..989 /label=conserved RBS 982..993 /label=BBa_B0034 CDS 1000..2170 /label=TetX misc_feature 2171..2214 /label=protein linker CDS 2215..2934 /label=GFP CDS 1000..2934 /label=BBa_K2150013 misc_feature 2947..2950 /label=conserved RBS 2943..2954 /label=BBa_B0034 promoter 5458..5485 /label=putative E. coli promoter CDS 2961..5615 /label=BBa_I2032 stem_loop 5635..5678 /label=stem_loop misc_feature 5624..5703 /label=BBa_B0010 terminator 5624..5703 /label=BBa_B0010 stem_loop 5719..5738 /label=T7 TE misc_feature 5745..5745 /label=stop polyA_site 5739..5752 /label=polya misc_feature 5712..5752 /label=BBa_B0012 terminator 5712..5752 /label=BBa_B0012 terminator 5624..5752 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaatacgac 901 tcactatagg gtactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag 961 agatactgag cactactaga gaaagaggag aaatactaga tgaaaaaaac catgcgtatc 1021 gacaccgaca aacagatgaa cctgctgtct gacaaaaacg ttgcgatcat cggtggtggt 1081 ccggttggtc tgaccatggc gaaactgctt cagcagaacg gtatcgacgt ttctgtttac 1141 gaacgtgaca acgaccgtga agcgcgtatc ttcggtggta ccctggacct gcacaaaggt 1201 tctggtcagg aagcgatgaa aaaagcgggt ctgcttcaga cctactacga cctggcgctg 1261 ccgatgggtg ttaacatcgc ggacaaaaaa ggtaacatcc tgtctaccaa aaacgttaaa 1321 ccggaaaacc gtttcgacaa cccggaaatc aaccgtaacg acctgcgtgc gatcctgctg 1381 aactctctgg aaaacgacac cgttatctgg gaccgtaaac tggttatgct ggaaccgggt 1441 aaaaaaaaat ggaccctgac cttcgaaaac aaaccgtctg aaaccgcgga cctggttatc 1501 ctggcgaacg gtggtatgtc taaagttcgt aaattcgtta ccgacaccga agttgaagaa 1561 accggtacct tcaacatcca ggcggacatc caccagccgg aaatcaactg cccgggtttc 1621 ttccagctgt gcaacggtaa ccgtctgatg gcgtctcacc agggtaacct gctgttcgcg 1681 aacccgaaca acaacggtgc gctgcacttc ggtatctctt tcaaaacccc ggacgaatgg 1741 aaaaaccaga cccaggttga cttccagaac cgtaactctg ttgttgactt cctgctgaaa 1801 gagttctctg actgggacga acgttacaaa gaactgatcc acaccaccct gtctttcgtt 1861 ggtctggcga cccgtatctt cccgctggaa aaaccgtgga aatctaaacg tccgctgccg 1921 atcaccatga tcggtgacgc ggcgcacctg atgccgccgt tcgcgggtca gggtgttaac 1981 tctggtctgg ttgacgcgct gatcctgtct gacaacctgg cggacggtaa attcaactct 2041 atcgaagaag cggttaaaaa ctacgaacag cagatgttca tgtacggtaa agaagcgcag 2101 gaagaatcta cccagaacga aatcgaaatg ttcaaaccgg acttcacctt ccagcagctg 2161 ctgaacgttg gtggaggagg ttctggaggc ggtggaagtg gtggcggagg tagcatgcgt 2221 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 2281 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 2341 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 2401 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 2461 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 2521 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 2581 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 2641 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 2701 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 2761 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 2821 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 2881 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag 2941 agaaagagga gaaatactag atgaacacga ttaacatcgc taagaacgac ttctctgaca 3001 tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc 3061 gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga 3121 tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca 3181 tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag 3241 ctaagcgcgg caagcgcccg acagccttcc agttcctgca agaaatcaag ccggaagccg 3301 tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg 3361 ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta 3421 tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg 3481 tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg 3541 gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag 3601 tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg 3661 ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta 3721 tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc 3781 ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc 3841 tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc 3901 ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag 3961 tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg 4021 cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc 4081 tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc 4141 gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca 4201 tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc 4261 cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta 4321 aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc 4381 cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta 4441 agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt 4501 tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc 4561 tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg 4621 taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg 4681 ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag 4741 tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta 4801 aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag 4861 tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata 4921 ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg 4981 ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg 5041 aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga 5101 agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc 5161 ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc 5221 agttccgctt acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac 5281 aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga 5341 ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct 5401 tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg 5461 acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc 5521 acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg 5581 acatcttaga gtcggacttc gcgttcgcgt aataatacta gagccaggca tcaaataaaa 5641 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 5701 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //