##gff-version 3
##sequence-region BBa_K2150027 1 5752
BBa_K2150027	.	promoter	920	973	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2150027	.	engineered_region	920	973	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2150027	.	promoter	962	967	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2150027	.	non_covalent_binding_site	920	938	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2150027	.	promoter	939	944	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2150027	.	non_covalent_binding_site	945	963	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2150027	.	CDS	2961	5615	.	+	0	ID=BBa_I2032;Name=BBa_I2032
BBa_K2150027	.	promoter	5458	5485	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
BBa_K2150027	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150027	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150027	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150027	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150027	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150027	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150027	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150027	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150027	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150027	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150027	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150027	.	ribosome_entry_site	2943	2954	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150027	.	conserved_region	2947	2950	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150027	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K2150027	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K2150027	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K2150027	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K2150027	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K2150027	.	ribosome_entry_site	982	993	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150027	.	conserved_region	986	989	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150027	.	CDS	1000	2934	.	+	0	ID=BBa_K2150013;Name=BBa_K2150013
BBa_K2150027	.	CDS	1000	2170	.	+	0	ID=annotation2489537;Name=TetX;Parent=BBa_K2150013
BBa_K2150027	.	CDS	2215	2934	.	+	0	ID=annotation2489539;Name=GFP;Parent=BBa_K2150013
BBa_K2150027	.	tag	2171	2214	.	+	0	ID=annotation2489538;Name=protein linker;Parent=BBa_K2150013
BBa_K2150027	.	terminator	5624	5752	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150027	.	terminator	5712	5752	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150027	.	engineered_region	5712	5752	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150027	.	polyA_site	5739	5752	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150027	.	stem_loop	5719	5738	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150027	.	stop_codon	5745	5745	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150027	.	terminator	5624	5703	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150027	.	engineered_region	5624	5703	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150027	.	stem_loop	5635	5678	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150027	.	promoter	892	911	.	+	0	ID=BBa_K2150031;Name=BBa_K2150031
>BBa_K2150027
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtaatacgactcactatagggtactagagtccctatcagtgatagagattgacatccc
tatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaaaaccatgcgtatcgacaccg
acaaacagatgaacctgctgtctgacaaaaacgttgcgatcatcggtggtggtccggttggtctgaccatggcgaaact
gcttcagcagaacggtatcgacgtttctgtttacgaacgtgacaacgaccgtgaagcgcgtatcttcggtggtaccctg
gacctgcacaaaggttctggtcaggaagcgatgaaaaaagcgggtctgcttcagacctactacgacctggcgctgccga
tgggtgttaacatcgcggacaaaaaaggtaacatcctgtctaccaaaaacgttaaaccggaaaaccgtttcgacaaccc
ggaaatcaaccgtaacgacctgcgtgcgatcctgctgaactctctggaaaacgacaccgttatctgggaccgtaaactg
gttatgctggaaccgggtaaaaaaaaatggaccctgaccttcgaaaacaaaccgtctgaaaccgcggacctggttatcc
tggcgaacggtggtatgtctaaagttcgtaaattcgttaccgacaccgaagttgaagaaaccggtaccttcaacatcca
ggcggacatccaccagccggaaatcaactgcccgggtttcttccagctgtgcaacggtaaccgtctgatggcgtctcac
cagggtaacctgctgttcgcgaacccgaacaacaacggtgcgctgcacttcggtatctctttcaaaaccccggacgaat
ggaaaaaccagacccaggttgacttccagaaccgtaactctgttgttgacttcctgctgaaagagttctctgactggga
cgaacgttacaaagaactgatccacaccaccctgtctttcgttggtctggcgacccgtatcttcccgctggaaaaaccg
tggaaatctaaacgtccgctgccgatcaccatgatcggtgacgcggcgcacctgatgccgccgttcgcgggtcagggtg
ttaactctggtctggttgacgcgctgatcctgtctgacaacctggcggacggtaaattcaactctatcgaagaagcggt
taaaaactacgaacagcagatgttcatgtacggtaaagaagcgcaggaagaatctacccagaacgaaatcgaaatgttc
aaaccggacttcaccttccagcagctgctgaacgttggtggaggaggttctggaggcggtggaagtggtggcggaggta
gcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatc
gaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagc
atgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataa
cgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaa
gctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccatta
agaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattga
ggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaag
cgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcg
aggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccgg
aatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgct
gaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgt
ggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagc
actgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatc
aacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagc
gtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgc
tgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgct
aaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaag
gtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaat
ccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaac
atcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttct
gctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctc
tggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgtt
caggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaag
tagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatg
gctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttc
cgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcagg
ctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggct
taagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtg
cattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcc
tcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtat
cgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatc
gaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaa
ctatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctca
attggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcg
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata