##gff-version 3
##sequence-region BBa_K2150028 1 4916
BBa_K2150028	.	CDS	2845	4779	.	+	0	ID=BBa_K2150013;Name=BBa_K2150013
BBa_K2150028	.	CDS	2845	4015	.	+	0	ID=annotation2489537;Name=TetX;Parent=BBa_K2150013
BBa_K2150028	.	tag	4016	4059	.	+	0	ID=annotation2489538;Name=protein linker;Parent=BBa_K2150013
BBa_K2150028	.	CDS	4060	4779	.	+	0	ID=annotation2489539;Name=GFP;Parent=BBa_K2150013
BBa_K2150028	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150028	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150028	.	promoter	2799	2818	.	+	0	ID=BBa_K2150031;Name=BBa_K2150031
BBa_K2150028	.	CDS	81	2735	.	+	0	ID=BBa_I2032;Name=BBa_I2032
BBa_K2150028	.	promoter	2578	2605	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
BBa_K2150028	.	ribosome_entry_site	2827	2838	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150028	.	conserved_region	2831	2834	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150028	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2150028	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2150028	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2150028	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2150028	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2150028	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2150028	.	terminator	4788	4916	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150028	.	terminator	4876	4916	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150028	.	engineered_region	4876	4916	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150028	.	stem_loop	4883	4902	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150028	.	polyA_site	4903	4916	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150028	.	stop_codon	4909	4909	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150028	.	terminator	4788	4867	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150028	.	engineered_region	4788	4867	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150028	.	stem_loop	4799	4842	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150028	.	terminator	2744	2790	.	+	0	ID=BBa_B0013;Name=BBa_B0013
BBa_K2150028	.	sequence_alteration	2780	2780	.	+	0	ID=annotation1695;Name=C;Parent=BBa_B0013
BBa_K2150028	.	polyA_site	2744	2749	.	+	0	ID=annotation1697;Name='';Parent=BBa_B0013
BBa_K2150028	.	engineered_region	2744	2790	.	+	0	ID=annotation7021;Name=BBa_B0013;Parent=BBa_B0013
BBa_K2150028	.	sequence_alteration	2753	2753	.	+	0	ID=annotation1696;Name=C;Parent=BBa_B0013
BBa_K2150028	.	stem_loop	2757	2776	.	+	0	ID=annotation1691;Name=T7 TE;Parent=BBa_B0013
BBa_K2150028	.	stop_codon	2783	2783	.	+	0	ID=annotation1692;Name=stop;Parent=BBa_B0013
BBa_K2150028	.	polyA_site	2777	2790	.	+	0	ID=annotation1698;Name=polya;Parent=BBa_B0013
>BBa_K2150028
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgac
cattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgca
agatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccc
taagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttc
ctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaata
caaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttga
agctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaa
gttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattc
atgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagt
aggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggc
atctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacg
gtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggt
gtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcacc
aagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgaca
tgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccg
tatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggac
tggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcga
aaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttcc
gttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacact
tggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctga
gctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatga
ggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaac
gagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatct
ctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcg
ttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagct
attgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctg
tgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaa
agataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcag
gaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacacca
acaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagcca
ccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtacc
attccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactgg
ctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaa
cttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagaaaaaatcaaactggctcacct
tcgggtgggcctttttgcgtttatatactagagtaatacgactcactatagggtactagagaaagaggagaaatactag
atgaaaaaaaccatgcgtatcgacaccgacaaacagatgaacctgctgtctgacaaaaacgttgcgatcatcggtggtg
gtccggttggtctgaccatggcgaaactgcttcagcagaacggtatcgacgtttctgtttacgaacgtgacaacgaccg
tgaagcgcgtatcttcggtggtaccctggacctgcacaaaggttctggtcaggaagcgatgaaaaaagcgggtctgctt
cagacctactacgacctggcgctgccgatgggtgttaacatcgcggacaaaaaaggtaacatcctgtctaccaaaaacg
ttaaaccggaaaaccgtttcgacaacccggaaatcaaccgtaacgacctgcgtgcgatcctgctgaactctctggaaaa
cgacaccgttatctgggaccgtaaactggttatgctggaaccgggtaaaaaaaaatggaccctgaccttcgaaaacaaa
ccgtctgaaaccgcggacctggttatcctggcgaacggtggtatgtctaaagttcgtaaattcgttaccgacaccgaag
ttgaagaaaccggtaccttcaacatccaggcggacatccaccagccggaaatcaactgcccgggtttcttccagctgtg
caacggtaaccgtctgatggcgtctcaccagggtaacctgctgttcgcgaacccgaacaacaacggtgcgctgcacttc
ggtatctctttcaaaaccccggacgaatggaaaaaccagacccaggttgacttccagaaccgtaactctgttgttgact
tcctgctgaaagagttctctgactgggacgaacgttacaaagaactgatccacaccaccctgtctttcgttggtctggc
gacccgtatcttcccgctggaaaaaccgtggaaatctaaacgtccgctgccgatcaccatgatcggtgacgcggcgcac
ctgatgccgccgttcgcgggtcagggtgttaactctggtctggttgacgcgctgatcctgtctgacaacctggcggacg
gtaaattcaactctatcgaagaagcggttaaaaactacgaacagcagatgttcatgtacggtaaagaagcgcaggaaga
atctacccagaacgaaatcgaaatgttcaaaccggacttcaccttccagcagctgctgaacgttggtggaggaggttct
ggaggcggtggaagtggtggcggaggtagcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttg
aattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttac
ccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaa
tgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaa
gaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatag
aatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacac
aatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaa
gcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacct
gtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgct
gggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata