BBa_K2150028
type: ComponentDefinition
persistent identity: BBa K2150028
display id: BBa_K2150028
version: 1
was generated by: iGEM to SBOL conversion
description: pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T10:23:30Z
mutable provenance: T7RNAP:PCR amplified from Genome of BL21. pT7:PCR amplified from BBa_K525998. As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).
owned by: james
owned by: myers
mutable description: In this part, the first promoter pTet is constitutively on, producing T7 RNA polymerase(T7RNAP). T7RNAP can activate the T7 promoter, thus producing the fusion protein in large quantity. The fusion protein(referred to as tetX-GFP) of tetX(a tetracycline-degrading enzyme) and GFP combined with a 3*GGGGS linker is used to report the expression level of tetX.
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29891
owner id: 31283
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Two different terminators are used to avoid repeated sequence. Linker for fusion protein is carefully designed to avoid repeated sequence which may cause problems in assembly.
star: false
creator: Jianyi Huang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I2032
component: BBa_B0034
component: BBa_K2150031
component: BBa_B0013
component: BBa_K2150013
component: BBa_B0015
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_I2032
sequence annotation: BBa_B0013
sequence annotation: BBa_K2150031
sequence annotation: BBa_K2150013
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2150028_sequence/1

BBa_I2032
type: Component
persistent identity: component2496137
display id: component2496137
version: 1
top level: BBa_K2150028
definition: BBa_I2032
access: public

BBa_B0034
type: Component
persistent identity: component2496135
display id: component2496135
version: 1
top level: BBa_K2150028
definition: BBa_B0034
access: public

BBa_K2150031
type: Component
persistent identity: component2496145
display id: component2496145
version: 1
top level: BBa_K2150028
definition: pT7
access: public

BBa_B0013
type: Component
persistent identity: component2496139
display id: component2496139
version: 1
top level: BBa_K2150028
definition: BBa_B0013
access: public

BBa_K2150013
type: Component
persistent identity: component2496151
display id: component2496151
version: 1
top level: BBa_K2150028
definition: tetX-GFP
access: public

BBa_B0034
type: Component
persistent identity: component2496147
display id: component2496147
version: 1
top level: BBa_K2150028
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2496158
display id: component2496158
version: 1
top level: BBa_K2150028
definition: BBa_B0015
access: public

BBa_R0040
type: Component
persistent identity: component2496129
display id: component2496129
version: 1
top level: BBa_K2150028
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2496129
display id: annotation2496129
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496129/range2496129/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496129/range2496129/1
type: Range
persistent identity: range2496129
display id: range2496129
version: 1
top level: BBa_K2150028
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2496135
display id: annotation2496135
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496135/range2496135/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496135/range2496135/1
type: Range
persistent identity: range2496135
display id: range2496135
version: 1
top level: BBa_K2150028
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_I2032
type: SequenceAnnotation
persistent identity: annotation2496137
display id: annotation2496137
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496137/range2496137/1
component: BBa_I2032

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496137/range2496137/1
type: Range
persistent identity: range2496137
display id: range2496137
version: 1
top level: BBa_K2150028
direction: direction/reverse
start: 81
end: 2735
orientation: inline

BBa_B0013
type: SequenceAnnotation
persistent identity: annotation2496139
display id: annotation2496139
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496139/range2496139/1
component: BBa_B0013

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496139/range2496139/1
type: Range
persistent identity: range2496139
display id: range2496139
version: 1
top level: BBa_K2150028
direction: direction/forward
start: 2744
end: 2790
orientation: inline

BBa_K2150031
type: SequenceAnnotation
persistent identity: annotation2496145
display id: annotation2496145
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496145/range2496145/1
component: BBa_K2150031

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496145/range2496145/1
type: Range
persistent identity: range2496145
display id: range2496145
version: 1
top level: BBa_K2150028
direction: direction/reverse
start: 2799
end: 2818
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2496147
display id: annotation2496147
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496147/range2496147/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496147/range2496147/1
type: Range
persistent identity: range2496147
display id: range2496147
version: 1
top level: BBa_K2150028
direction: direction/reverse
start: 2827
end: 2838
orientation: inline

BBa_K2150013
type: SequenceAnnotation
persistent identity: annotation2496151
display id: annotation2496151
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496151/range2496151/1
component: BBa_K2150013

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496151/range2496151/1
type: Range
persistent identity: range2496151
display id: range2496151
version: 1
top level: BBa_K2150028
direction: direction/reverse
start: 2845
end: 4779
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2496158
display id: annotation2496158
version: 1
top level: BBa_K2150028
location: https://synbiohub.org/public/igem/BBa_K2150028/annotation2496158/range2496158/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2150028/annotation2496158/range2496158/1
type: Range
persistent identity: range2496158
display id: range2496158
version: 1
top level: BBa_K2150028
direction: direction/reverse
start: 4788
end: 4916
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I2032
type: ComponentDefinition
persistent identity: BBa I2032
display id: BBa_I2032
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA polymerase
created: 2007-04-11T11:00:00Z
modified: 2016-10-14T06:52:19Z
mutable provenance: Bacteriophage T7
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 27399
owner id: 135
owning group id: 84
sample status: In stock
status: status/Available
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: putative E. coli promoter
sequence: https://synbiohub.org/public/igem/BBa_I2032_sequence/1

putative E. coli promoter
type: SequenceAnnotation
persistent identity: annotation1928674
display id: annotation1928674
version: 1
top level: BBa_I2032
location: https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
type: Range
persistent identity: range1928674
display id: range1928674
version: 1
top level: BBa_I2032
direction: direction/forward
start: 2498
end: 2525
orientation: inline

tetX-GFP
type: ComponentDefinition
persistent identity: BBa K2150013
display id: BBa_K2150013
version: 1
was generated by: iGEM to SBOL conversion
description: A fusion protein combining tetX(BBa_K2150101) with GFP(BBa_E0040)
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T01:06:12Z
mutable provenance: The source of tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).
owned by: james
owned by: myers
mutable description: This protein(noted as tetX-GFP) combines a tetracycline-degrading enzyme (tetX monooxygenase, ) with a green fluorescent protein (GFPmt3, BBa_E0040). TetX-GFP has activities of both the tetX and the GFP. As a degradation enzyme, it can degrade tetracycline(tc) and its analogs as we describe (here), and also give the tetracycline resistance to E.coli. As a green fluorescent protein, it inherits the normal function of BBa_E0040. However, the expression quantity of this protein may be smaller than BBa_E0040 under the same promoter or it may take longer time for tetX-GFP to mature, for we notice that when it is expressed under the same promoter as BBa_E0040, the fluorescent intensity(FI) of tetX-GFP is weaker than BBa_E0040 at the same growth stage of E.coli, and it takes longer for E.coli expressing tetX-GFP to reach the same FI as E.coli expressing BBa_E0040. We use tetX-GFP as a monitor of the quantity of tetX expressed in E.coli. TetX-GFP can also act as a reporter of whether our system is working (meaning whether our machine is degrading tetracycline). When tetX-GFP is expressed under a tetracycline-regulated promoter such as BBa_R0040, it will become a visible sensor of tetracycline in the presence of tetR, with no need for additional tetracycline-resistance genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2620_
m user id: 29891
owner id: 29891
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The protein linker is 3*GGGGS which provides flexibility to the fusion protein. When choosing the codon for this linker, we need to avoid repeated sequence which may cause problems when assemble these parts.
star: false
creator: Yang Ye
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: TetX
sequence annotation: protein linker
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K2150013_sequence/1

TetX
type: SequenceAnnotation
persistent identity: annotation2489537
display id: annotation2489537
version: 1
top level: tetX-GFP
location: https://synbiohub.org/public/igem/BBa_K2150013/annotation2489537/range2489537/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K2150013/annotation2489537/range2489537/1
type: Range
persistent identity: range2489537
display id: range2489537
version: 1
top level: tetX-GFP
direction: direction/forward
start: 1
end: 1171
orientation: inline

protein linker
type: SequenceAnnotation
persistent identity: annotation2489538
display id: annotation2489538
version: 1
top level: tetX-GFP
location: https://synbiohub.org/public/igem/BBa_K2150013/annotation2489538/range2489538/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K2150013/annotation2489538/range2489538/1
type: Range
persistent identity: range2489538
display id: range2489538
version: 1
top level: tetX-GFP
direction: direction/forward
start: 1172
end: 1215
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2489539
display id: annotation2489539
version: 1
top level: tetX-GFP
location: https://synbiohub.org/public/igem/BBa_K2150013/annotation2489539/range2489539/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2150013/annotation2489539/range2489539/1
type: Range
persistent identity: range2489539
display id: range2489539
version: 1
top level: tetX-GFP
direction: direction/forward
start: 1216
end: 1935
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa K2150031
display id: BBa_K2150031
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter without RBS
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T09:06:12Z
mutable provenance: PCR amplified from BBa_K525998
owned by: james
owned by: myers
mutable description: This part is the T7 promoter without RBS. It has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase(T7RNAP). However, we observed leakage of it in the absence of T7RNAP.
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 31283
owner id: 31283
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We deleted the RBS in BBa_K525998, so that RBS with different efficiency can be used.
star: false
creator: Jianyi Huang
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K2150031_sequence/1

BBa_B0013
type: ComponentDefinition
persistent identity: BBa B0013
display id: BBa_B0013
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphage T7 (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ''
sequence annotation: BBa_B0013
sequence annotation: C
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0013_sequence/1

''
type: SequenceAnnotation
persistent identity: annotation1697
display id: annotation1697
version: 1
top level: BBa_B0013
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0013/annotation1697/range1697/1
type: Range
persistent identity: range1697
display id: range1697
version: 1
top level: BBa_B0013
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0013
type: SequenceAnnotation
persistent identity: annotation7021
display id: annotation7021
version: 1
top level: BBa_B0013
location: https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0013/annotation7021/range7021/1
type: Range
persistent identity: range7021
display id: range7021
version: 1
top level: BBa_B0013
direction: direction/forward
start: 1
end: 47
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1696
display id: annotation1696
version: 1
top level: BBa_B0013
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_B0013/annotation1696/range1696/1
type: Range
persistent identity: range1696
display id: range1696
version: 1
top level: BBa_B0013
direction: direction/forward
start: 10
end: 10
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1691
display id: annotation1691
version: 1
top level: BBa_B0013
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0013/annotation1691/range1691/1
type: Range
persistent identity: range1691
display id: range1691
version: 1
top level: BBa_B0013
direction: direction/forward
start: 14
end: 33
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1698
display id: annotation1698
version: 1
top level: BBa_B0013
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0013/annotation1698/range1698/1
type: Range
persistent identity: range1698
display id: range1698
version: 1
top level: BBa_B0013
direction: direction/forward
start: 34
end: 47
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1695
display id: annotation1695
version: 1
top level: BBa_B0013
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_B0013/annotation1695/range1695/1
type: Range
persistent identity: range1695
display id: range1695
version: 1
top level: BBa_B0013
direction: direction/forward
start: 37
end: 37
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1692
display id: annotation1692
version: 1
top level: BBa_B0013
location: https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0013/annotation1692/range1692/1
type: Range
persistent identity: range1692
display id: range1692
version: 1
top level: BBa_B0013
direction: direction/forward
start: 40
end: 40
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2150028_sequence/1
type: Sequence
persistent identity: BBa K2150028 sequence
display id: BBa_K2150028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I2032_sequence/1
type: Sequence
persistent identity: BBa I2032 sequence
display id: BBa_I2032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2150013_sequence/1
type: Sequence
persistent identity: BBa K2150013 sequence
display id: BBa_K2150013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaaccatgcgtatcgacaccgacaaacagatgaacctgctgtctgacaaaaacgttgcgatcatcggtggtggtccggttggtctgaccatggcgaaactgcttcagcagaacggtatcgacgtttctgtttacgaacgtgacaacgaccgtgaagcgcgtatcttcggtggtaccctggacctgcacaaaggttctggtcaggaagcgatgaaaaaagcgggtctgcttcagacctactacgacctggcgctgccgatgggtgttaacatcgcggacaaaaaaggtaacatcctgtctaccaaaaacgttaaaccggaaaaccgtttcgacaacccggaaatcaaccgtaacgacctgcgtgcgatcctgctgaactctctggaaaacgacaccgttatctgggaccgtaaactggttatgctggaaccgggtaaaaaaaaatggaccctgaccttcgaaaacaaaccgtctgaaaccgcggacctggttatcctggcgaacggtggtatgtctaaagttcgtaaattcgttaccgacaccgaagttgaagaaaccggtaccttcaacatccaggcggacatccaccagccggaaatcaactgcccgggtttcttccagctgtgcaacggtaaccgtctgatggcgtctcaccagggtaacctgctgttcgcgaacccgaacaacaacggtgcgctgcacttcggtatctctttcaaaaccccggacgaatggaaaaaccagacccaggttgacttccagaaccgtaactctgttgttgacttcctgctgaaagagttctctgactgggacgaacgttacaaagaactgatccacaccaccctgtctttcgttggtctggcgacccgtatcttcccgctggaaaaaccgtggaaatctaaacgtccgctgccgatcaccatgatcggtgacgcggcgcacctgatgccgccgttcgcgggtcagggtgttaactctggtctggttgacgcgctgatcctgtctgacaacctggcggacggtaaattcaactctatcgaagaagcggttaaaaactacgaacagcagatgttcatgtacggtaaagaagcgcaggaagaatctacccagaacgaaatcgaaatgttcaaaccggacttcaccttccagcagctgctgaacgttggtggaggaggttctggaggcggtggaagtggtggcggaggtagcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2150031_sequence/1
type: Sequence
persistent identity: BBa K2150031 sequence
display id: BBa_K2150031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0013_sequence/1
type: Sequence
persistent identity: BBa B0013 sequence
display id: BBa_B0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z