LOCUS BBa_K2150029 5807 bp DNA linear 26-Dec-2024 DEFINITION pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT ACCESSION BBa_K2150029 VERSION BBa_K2150029.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 misc_feature 958..961 /label=conserved RBS 954..965 /label=BBa_B0034 promoter 3469..3496 /label=putative E. coli promoter CDS 972..3626 /label=BBa_I2032 stem_loop 3648..3667 /label=T7 TE misc_feature 3674..3674 /label=stop misc_feature 3671..3671 /label=C misc_feature 3644..3644 /label=C polyA_site 3635..3640 /label='' polyA_site 3668..3681 /label=polya misc_feature 3635..3681 /label=BBa_B0013 terminator 3635..3681 /label=BBa_B0013 promoter 3690..3709 /label=BBa_K2150031 misc_feature 3722..3725 /label=conserved RBS 3718..3729 /label=BBa_B0034 CDS 3736..4906 /label=TetX misc_feature 4907..4950 /label=protein linker CDS 4951..5670 /label=GFP CDS 3736..5670 /label=BBa_K2150013 stem_loop 5690..5733 /label=stem_loop misc_feature 5679..5758 /label=BBa_B0010 terminator 5679..5758 /label=BBa_B0010 stem_loop 5774..5793 /label=T7 TE misc_feature 5800..5800 /label=stop polyA_site 5794..5807 /label=polya misc_feature 5767..5807 /label=BBa_B0012 terminator 5767..5807 /label=BBa_B0012 terminator 5679..5807 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 961 agaaatacta gatgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg 1021 ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt 1081 tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc 1141 gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc 1201 tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg 1261 gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca 1321 tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg 1381 tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc 1441 ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg 1501 tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg 1561 gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca 1621 tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag 1681 taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc 1741 gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc 1801 cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg 1861 tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt 1921 acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg 1981 tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc 2041 gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt 2101 ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca 2161 gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc 2221 cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta 2281 acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt 2341 actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg 2401 agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac 2461 tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg 2521 agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg 2581 acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc 2641 gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga 2701 aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg 2761 tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg 2821 ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc 2881 tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc 2941 cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca 3001 tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga 3061 actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag 3121 agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc 3181 aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct 3241 tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg 3301 gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt 3361 gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca 3421 ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg 3481 agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc 3541 aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag 3601 agtcggactt cgcgttcgcg taataatact agagaaaaaa tcaaactggc tcaccttcgg 3661 gtgggccttt ttgcgtttat atactagagt aatacgactc actatagggt actagagaaa 3721 gaggagaaat actagatgaa aaaaaccatg cgtatcgaca ccgacaaaca gatgaacctg 3781 ctgtctgaca aaaacgttgc gatcatcggt ggtggtccgg ttggtctgac catggcgaaa 3841 ctgcttcagc agaacggtat cgacgtttct gtttacgaac gtgacaacga ccgtgaagcg 3901 cgtatcttcg gtggtaccct ggacctgcac aaaggttctg gtcaggaagc gatgaaaaaa 3961 gcgggtctgc ttcagaccta ctacgacctg gcgctgccga tgggtgttaa catcgcggac 4021 aaaaaaggta acatcctgtc taccaaaaac gttaaaccgg aaaaccgttt cgacaacccg 4081 gaaatcaacc gtaacgacct gcgtgcgatc ctgctgaact ctctggaaaa cgacaccgtt 4141 atctgggacc gtaaactggt tatgctggaa ccgggtaaaa aaaaatggac cctgaccttc 4201 gaaaacaaac cgtctgaaac cgcggacctg gttatcctgg cgaacggtgg tatgtctaaa 4261 gttcgtaaat tcgttaccga caccgaagtt gaagaaaccg gtaccttcaa catccaggcg 4321 gacatccacc agccggaaat caactgcccg ggtttcttcc agctgtgcaa cggtaaccgt 4381 ctgatggcgt ctcaccaggg taacctgctg ttcgcgaacc cgaacaacaa cggtgcgctg 4441 cacttcggta tctctttcaa aaccccggac gaatggaaaa accagaccca ggttgacttc 4501 cagaaccgta actctgttgt tgacttcctg ctgaaagagt tctctgactg ggacgaacgt 4561 tacaaagaac tgatccacac caccctgtct ttcgttggtc tggcgacccg tatcttcccg 4621 ctggaaaaac cgtggaaatc taaacgtccg ctgccgatca ccatgatcgg tgacgcggcg 4681 cacctgatgc cgccgttcgc gggtcagggt gttaactctg gtctggttga cgcgctgatc 4741 ctgtctgaca acctggcgga cggtaaattc aactctatcg aagaagcggt taaaaactac 4801 gaacagcaga tgttcatgta cggtaaagaa gcgcaggaag aatctaccca gaacgaaatc 4861 gaaatgttca aaccggactt caccttccag cagctgctga acgttggtgg aggaggttct 4921 ggaggcggtg gaagtggtgg cggaggtagc atgcgtaaag gagaagaact tttcactgga 4981 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 5041 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 5101 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc 5161 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa 5221 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 5281 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 5341 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 5401 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 5461 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 5521 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 5581 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca 5641 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa 5701 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 5761 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //