LOCUS       BBa_K2150030            1697 bp    DNA     linear       14-May-2024
DEFINITION  pCon+pTet+pLas+RBS+ydcD+DT+pCon+RBS+lasR+DT
ACCESSION   BBa_K2150030
VERSION     BBa_K2150030.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    44..62
                     /label=TetR 1
     misc_feature    44..97
                     /label=BBa_R0040
     promoter        63..68
                     /label=-35
     misc_feature    69..87
                     /label=TetR 2
     promoter        86..91
                     /label=-10
     promoter        44..97
                     /label=BBa_R0040
     misc_feature    151..168
                     /label=OP2
     misc_feature    211..230
                     /label=OP1
     promoter        222..227
                     /label=-35
     promoter        245..250
                     /label=-10
     promoter        106..262
                     /label=BBa_R0079
     misc_feature    275..278
                     /label=conserved
     RBS             271..282
                     /label=BBa_B0034
     CDS             289..573
                     /label=BBa_K2150032
     stem_loop       593..636
                     /label=stem_loop
     misc_feature    582..661
                     /label=BBa_B0010
     terminator      582..661
                     /label=BBa_B0010
     stem_loop       677..696
                     /label=T7 TE
     misc_feature    703..703
                     /label=stop
     polyA_site      697..710
                     /label=polya
     misc_feature    670..710
                     /label=BBa_B0012
     terminator      670..710
                     /label=BBa_B0012
     terminator      582..710
                     /label=BBa_B0015
     promoter        719..753
                     /label=BBa_J23119
     misc_feature    766..769
                     /label=conserved
     RBS             762..773
                     /label=BBa_B0034
     CDS             780..1496
                     /label=lasR
     misc_feature    1497..1529
                     /label=LVA
     misc_feature    1259..1259
                     /label=G
     misc_feature    1536..1560
                     /label=Help:Barcodes
     CDS             780..1560
                     /label=BBa_C0079
     stem_loop       1580..1623
                     /label=stem_loop
     misc_feature    1569..1648
                     /label=BBa_B0010
     terminator      1569..1648
                     /label=BBa_B0010
     stem_loop       1664..1683
                     /label=T7 TE
     misc_feature    1690..1690
                     /label=stop
     polyA_site      1684..1697
                     /label=polya
     misc_feature    1657..1697
                     /label=BBa_B0012
     terminator      1657..1697
                     /label=BBa_B0012
     terminator      1569..1697
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagtccctat cagtgataga
       61 gattgacatc cctatcagtg atagagatac tgagcactac tagaggcccc tcgctgagcg
      121 cgtcccggag ctgggggcaa cctagctgcc acctgctttt ctgctagcta ttccagcgaa
      181 aacatacaga tttccggcga aatcaaggct acctgccagt tctggcaggt ttggccgcgg
      241 gttctttttg gtacacgaaa gctactagag aaagaggaga aatactagat gtctgaatcc
      301 agcgcaagaa ccgaaatgaa aatcagcttg cccgaaaacc tagtagctga attggatggt
      361 gtagcgatgc gggagaaacg aagcagaaac gaactgatat cacaagcagt gagagcgtat
      421 gtcagcgaac gaacaactcg tcacaaccgt gatttgatga gacgcggcta tatggaaatg
      481 gcgaaaatca acctgaatat ttcttctgag gctcactttg cggagtgcga ggctgaaacg
      541 acagttgagc gcttagtcag cggaggataa tcatactaga gccaggcatc aaataaaacg
      601 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      661 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt
      721 gacagctagc tcagtcctag gtataatgct agctactaga gaaagaggag aaatactaga
      781 tggccttggt tgacggtttt cttgagctgg aacgctcaag tggaaaattg gagtggagcg
      841 ccatcctcca gaagatggcg agcgaccttg gattctcgaa gatcctgttc ggcctgttgc
      901 ctaaggacag ccaggactac gagaacgcct tcatcgtcgg caactacccg gccgcctggc
      961 gcgagcatta cgaccgggct ggctacgcgc gggtcgaccc gacggtcagt cactgtaccc
     1021 agagcgtact gccgattttc tgggaaccgt ccatctacca gacgcgaaag cagcacgagt
     1081 tcttcgagga agcctcggcc gccggcctgg tgtatgggct gaccatgccg ctgcatggtg
     1141 ctcgcggcga actcggcgcg ctgagcctca gcgtggaagc ggaaaaccgg gccgaggcca
     1201 accgtttcat agagtcggtc ctgccgaccc tgtggatgct caaggactac gcactgcaaa
     1261 gcggtgccgg actggccttc gaacatccgg tcagcaaacc ggtggttctg accagccggg
     1321 agaaggaagt gttgcagtgg tgcgccatcg gcaagaccag ttgggagata tcggttatct
     1381 gcaactgctc ggaagccaat gtgaacttcc atatgggaaa tattcggcgg aagttcggtg
     1441 tgacctcccg ccgcgtagcg gccattatgg ccgttaattt gggtcttatt actctcgctg
     1501 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
     1561 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1621 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1681 gcctttctgc gtttata
//