##gff-version 3
##sequence-region BBa_K2150030 1 1697
BBa_K2150030	.	promoter	44	97	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2150030	.	promoter	86	91	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2150030	.	non_covalent_binding_site	44	62	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2150030	.	engineered_region	44	97	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2150030	.	non_covalent_binding_site	69	87	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2150030	.	promoter	63	68	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2150030	.	terminator	582	710	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150030	.	terminator	670	710	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150030	.	polyA_site	697	710	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150030	.	stem_loop	677	696	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150030	.	stop_codon	703	703	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150030	.	engineered_region	670	710	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150030	.	terminator	582	661	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150030	.	stem_loop	593	636	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150030	.	engineered_region	582	661	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150030	.	ribosome_entry_site	271	282	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150030	.	conserved_region	275	278	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150030	.	terminator	1569	1697	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150030	.	terminator	1657	1697	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150030	.	polyA_site	1684	1697	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150030	.	stem_loop	1664	1683	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150030	.	stop_codon	1690	1690	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150030	.	engineered_region	1657	1697	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150030	.	terminator	1569	1648	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150030	.	stem_loop	1580	1623	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150030	.	engineered_region	1569	1648	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2150030	.	promoter	106	262	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K2150030	.	non_covalent_binding_site	151	168	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K2150030	.	promoter	222	227	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K2150030	.	non_covalent_binding_site	211	230	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K2150030	.	promoter	245	250	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K2150030	.	ribosome_entry_site	762	773	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150030	.	conserved_region	766	769	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150030	.	promoter	719	753	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K2150030	.	CDS	289	573	.	+	0	ID=BBa_K2150032;Name=BBa_K2150032
BBa_K2150030	.	CDS	780	1560	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_K2150030	.	sequence_alteration	1259	1259	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K2150030	.	tag	1497	1529	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K2150030	.	CDS	780	1496	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K2150030	.	engineered_tag	1536	1560	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K2150030	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
>BBa_K2150030
ttgacagctagctcagtcctaggtataatgctagctactagagtccctatcagtgatagagattgacatccctatcagt
gatagagatactgagcactactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgctt
ttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccg
cgggttctttttggtacacgaaagctactagagaaagaggagaaatactagatgtctgaatccagcgcaagaaccgaaa
tgaaaatcagcttgcccgaaaacctagtagctgaattggatggtgtagcgatgcgggagaaacgaagcagaaacgaact
gatatcacaagcagtgagagcgtatgtcagcgaacgaacaactcgtcacaaccgtgatttgatgagacgcggctatatg
gaaatggcgaaaatcaacctgaatatttcttctgaggctcactttgcggagtgcgaggctgaaacgacagttgagcgct
tagtcagcggaggataatcatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggccttggt
tgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgacctt
ggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacc
cggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgt
actgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggc
ctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaa
accgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcgg
tgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgc
gccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaata
ttcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgc
aaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata