LOCUS       BBa_K215010             1662 bp    DNA     linear       29-Nov-2024
DEFINITION  pLac+RBS+GFP with Secretion Signal and Streptavidin Binding Tags 
            on GFP
ACCESSION   BBa_K215010
VERSION     BBa_K215010.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..196
                     /label=K215012
     CDS             197..913
                     /label=E0040
     CDS             914..1662
                     /label=K215012
     CDS             82..1662
                     /label=BBa_K215012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggacgtt gaagcatggc tgggtgcccg tgtaccgctg
      121 gttgaaacgg gcagcgcaca tcaccaccat catcatggtt ccgccgaaaa cctctacttt
      181 cagggcggct ctgctgctag acgtaaagga gaagaacttt tcactggagt tgtcccaatt
      241 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      301 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      361 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      421 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      481 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      541 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      601 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      661 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      721 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      781 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      841 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      901 gaactataca aaactagcgg ttctgccgag aatctgtact tccaaggtgg ctccgctcat
      961 caccaccatc atcacggtag cgcaggcggc actgacacct ttgacttctc tggttactct
     1021 aacaatcagc gtatcaacct gaacgagggt tctttctctg acgttggtgg tctgaagggc
     1081 aacgtttcta tcgcgcatgg tgttaccatc gaaaacgcga tcggtggttc tggtaacgac
     1141 atcctgatcg gtaatggcgc ggataacatc ctccagggtg gtgcgggtga cgacgttctg
     1201 tacggttcta cgggtgcgga cacgctgacg ggtggcgctg gtcgtgacat tttcgtatat
     1261 ggtagcggtc aagactctac cgtttctgca tacgactgga tcacggattt tcagacgggt
     1321 atcgacaaaa tcgacctgtc cgctttccgt aatgaaggtc agctgtcttt tgttcaggac
     1381 cagtttactg gtaaaggcca ggaggttatg ctgcaatggg acgcggcgaa ctctacgacc
     1441 aatctgtggc tgcacgaagc gggtcactct tctgttgact ttctcgttcg tatcgttggc
     1501 cagaccgcgc agagcgacat tatcgtctaa taaccaggca tcaaataaaa cgaaaggctc
     1561 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1621 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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