LOCUS       BBa_K2150110            2040 bp    DNA     linear       22-Dec-2024
DEFINITION  LacI (constitutive) + Ptac + RiboJ + 1204 toxin
ACCESSION   BBa_K2150110
VERSION     BBa_K2150110.1  
FEATURES             Location/Qualifiers
     CDS             1..1082
                     /label=lacIq
     promoter        1325..1352
                     /label=Ptac
     promoter        1..1447
                     /label=BBa_K180000
     promoter        1448..1522
                     /label=BBa_K1679038
     RBS             1523..1533
                     /label=RBS-4\Weaker
     RBS             1529..1532
                     /label=RBS
     misc_feature    1523..1533
                     /label=BBa_B0033
     RBS             1523..1533
                     /label=BBa_B0033
     CDS             1534..2034
                     /label=CDS of N-acetyltransferase GCN5
     misc_feature    2035..2039
                     /label=double stop codon
     CDS             1534..2040
                     /label=BBa_K2150103
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac
     1141 agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
     1201 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
     1261 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
     1321 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc
     1441 atagcattta aacaaaatta tttgtagagg ctgtttcgtc ctcacggact catcagaccg
     1501 gaaagcacat ccggtgacag cttcacacag gacatgagtt ccacgatgtt tgatgttatg
     1561 caactcaacg aagaacacga tcgtgattcg ttctacagtg gttcagaatt tctagatggt
     1621 tactttcaga gacaggttgg tcaggatgtt aggtgccgcg tagcagcttg ttttgttgcg
     1681 cttcatgatc accgtgtagc gggatattat actctggctg ctgccggtat ccagttggcc
     1741 aacctcccga cagctactat taagaaattg ccacgatacc caacagtgcc ggcagtacgg
     1801 atggggaggt tggccgttga tcaggcattt agaaatcagg gactcggtgg agcattgcta
     1861 gctgatgcta ttgagaggac cattcgtgcc gaaattgcat cttatgcgct gcttgttgac
     1921 gcaaaagatg ataatgcagt gcaattttac cgacatcatg gttttattgc tttacccgat
     1981 tcgccattca cattattcct tcctctcatc gctgtagata aaaaaggtaa aaaataataa
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