BBa_K2150120
1
BBa_K2150120
136 toxin aTc
2016-10-12T11:00:00Z
2016-10-18T07:06:08Z
a
a
false
false
_2620_
29734
29734
9
false
a
false
Zepeng Mu
component2520050
1
BBa_B0033
component2520052
1
BBa_K2150102
component2520043
1
BBa_R0040
component2520048
1
BBa_K1679038
annotation2520043
1
BBa_R0040
range2520043
1
1
54
annotation2520050
1
BBa_B0033
range2520050
1
146
156
annotation2520052
1
BBa_K2150102
range2520052
1
163
906
annotation2520048
1
BBa_K1679038
range2520048
1
63
137
BBa_K1679038
1
BBa_K1679038
RiboJ
2015-09-17T11:00:00Z
2015-09-18T01:15:04Z
Synthesized by Genewiz.
After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin immediately downstream to help expose the RBS. STRSV can cleaves the mRNA autocatalytically at a defined residue, which had the effect of cleaving extraneous RNA leaders that arose from transcription from promoters with different start sites.[1] Attributed to the biochemical function mentioned above, RiboJ has insulating capability. We use it to buffer synthetic circuits from genetic context.
[1] Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.
false
false
_2097_
25072
25072
9
false
After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin. Users can insert it into promoter and RBS to reduce noise caused by upstream sequence and stabilize mRNA.
false
Xihan Zhang
BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7028
1
BBa_B0033
range7028
1
1
11
annotation1714
1
RBS
range1714
1
7
10
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
BBa_K2150102
1
yobR CDS
136 toxin (yobR) CDS
2016-10-11T11:00:00Z
2016-10-12T11:44:11Z
a
a
false
false
_2620_
29734
29734
9
false
a
false
Zepeng Mu
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986785
1
-35
range1986785
1
20
25
BBa_B0033_sequence
1
tcacacaggac
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K2150102_sequence
1
atggatgacattcgcaagattgaacgattagcggcagcttcatggccggcttattttcaaaaaagcatagggaaatggcttctcagagcgaattttggcgtaaccaaaagggcaaatagcgtatggacgtctgcggatatgcctgaaggggattttcagctagaggccgaacttttttatcaaagtttaggcttacctgtttgttttcatattagcaacgcatcaccgaaagggcttgatgacgcattggcagacagccgttatgaaaaggtagacgagtgcttccagatgacagcgttgtgccggtcaattatgagccgaacaaatgacaacagccgatttacatacaagtgggaacaagagccatcatctgtttggatagatgaatttattcaattagaagggttttcacccgagcgtcacaaaggttacaaacacatattcgaacgcatgccgccgtgtaaaacttttttcaagatgtatgacaaagaaagcctgacagcgcttggcacggtttctgtcattgatggttatggcggacttagtaatatcgttgtagctgaggagcacagaggaaaaggggcaggaacccaagtgatcagggtgttgactgaatgggcaaaaaacaacggagcggaacgcatgtttttacaagtgatgaaagaaaatctggcggcagtttctttatatggaaaaatcggcttttcgcctatatcggaacaccattaccggattaaaagataa
BBa_K2150120_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagttaaacaaaattatttgtagaggctgtttcgtcctcacggactcatcagaccggaaagcacatccggtgacagcttactagagtcacacaggactactagatggatgacattcgcaagattgaacgattagcggcagcttcatggccggcttattttcaaaaaagcatagggaaatggcttctcagagcgaattttggcgtaaccaaaagggcaaatagcgtatggacgtctgcggatatgcctgaaggggattttcagctagaggccgaacttttttatcaaagtttaggcttacctgtttgttttcatattagcaacgcatcaccgaaagggcttgatgacgcattggcagacagccgttatgaaaaggtagacgagtgcttccagatgacagcgttgtgccggtcaattatgagccgaacaaatgacaacagccgatttacatacaagtgggaacaagagccatcatctgtttggatagatgaatttattcaattagaagggttttcacccgagcgtcacaaaggttacaaacacatattcgaacgcatgccgccgtgtaaaacttttttcaagatgtatgacaaagaaagcctgacagcgcttggcacggtttctgtcattgatggttatggcggacttagtaatatcgttgtagctgaggagcacagaggaaaaggggcaggaacccaagtgatcagggtgttgactgaatgggcaaaaaacaacggagcggaacgcatgtttttacaagtgatgaaagaaaatctggcggcagtttctttatatggaaaaatcggcttttcgcctatatcggaacaccattaccggattaaaagataa
BBa_K1679038_sequence
1
ttaaacaaaattatttgtagaggctgtttcgtcctcacggactcatcagaccggaaagcacatccggtgacagct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z