LOCUS       BBa_K2150125            1221 bp    DNA     linear       27-Jun-2024
DEFINITION  Ptet + RBS + RiboJ + toxin 5980 CDS
ACCESSION   BBa_K2150125
VERSION     BBa_K2150125.1  
FEATURES             Location/Qualifiers
     CDS             4..620
                     /label=tetR
     misc_feature    621..654
                     /label=SsrA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             1..685
                     /label=BBa_C0040
     misc_feature    694..712
                     /label=TetR 1
     misc_feature    694..747
                     /label=BBa_R0040
     promoter        713..718
                     /label=-35
     misc_feature    719..737
                     /label=TetR 2
     promoter        736..741
                     /label=-10
     promoter        694..747
                     /label=BBa_R0040
     promoter        756..830
                     /label=BBa_K1679038
     RBS             839..849
                     /label=RBS-4\Weaker
     RBS             845..848
                     /label=RBS
     misc_feature    839..849
                     /label=BBa_B0033
     RBS             839..849
                     /label=BBa_B0033
     CDS             856..1221
                     /label=5980 toxin CDS
     CDS             856..1221
                     /label=BBa_K2150122
ORIGIN
        1 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
       61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
      541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
      601 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa
      661 cactgatagt gctagtgtag atcactacta gagtccctat cagtgataga gattgacatc
      721 cctatcagtg atagagatac tgagcactac tagagttaaa caaaattatt tgtagaggct
      781 gtttcgtcct cacggactca tcagaccgga aagcacatcc ggtgacagct tactagagtc
      841 acacaggact actagatgac cttacagtta attagtgcag aagaaattat tcagtttcat
      901 gatcgcttac tgcgtgtgac gcctggcgtt accggtatgc ccgacccgga acgtgccgaa
      961 gcactgatgt atcgcgtgct gaatcagatt gaatatgaag gtgtgaccga tgtgtggctg
     1021 ttagcagcca tgcatctgtt aaccattagt cgcggctata tttttaatga tggcaacaag
     1081 cgtaccgccc tgtttattac cctgctgttt ctgaaacgta atggtatttc tctggcagcc
     1141 aatccggatt ttgtggatat gaccgttgat gccgccgcag gccgcttaac cttagaacag
     1201 attgcagttc gtctgcgcgc a
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