LOCUS       BBa_K2150126            2175 bp    DNA     linear       16-Jun-2024
DEFINITION  Ptet + RiboJ + RBS + toxin 4222 CDS
ACCESSION   BBa_K2150126
VERSION     BBa_K2150126.1  
FEATURES             Location/Qualifiers
     CDS             4..620
                     /label=tetR
     misc_feature    621..654
                     /label=SsrA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             1..685
                     /label=BBa_C0040
     misc_feature    694..712
                     /label=TetR 1
     misc_feature    694..747
                     /label=BBa_R0040
     promoter        713..718
                     /label=-35
     misc_feature    719..737
                     /label=TetR 2
     promoter        736..741
                     /label=-10
     promoter        694..747
                     /label=BBa_R0040
     promoter        756..830
                     /label=BBa_K1679038
     RBS             839..849
                     /label=RBS-4\Weaker
     RBS             845..848
                     /label=RBS
     misc_feature    839..849
                     /label=BBa_B0033
     RBS             839..849
                     /label=BBa_B0033
     CDS             856..2175
                     /label=toxin 4222 CDS
     CDS             856..2175
                     /label=BBa_K2150121
ORIGIN
        1 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
       61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
      541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
      601 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa
      661 cactgatagt gctagtgtag atcactacta gagtccctat cagtgataga gattgacatc
      721 cctatcagtg atagagatac tgagcactac tagagttaaa caaaattatt tgtagaggct
      781 gtttcgtcct cacggactca tcagaccgga aagcacatcc ggtgacagct tactagagtc
      841 acacaggact actagatgac gagcaaagcc gatgcgaccg aagcatttgt gtggatttgg
      901 ttaccgggcg cgaccgaacc agttgttgcg ggtcgcctgt ttcaggaagg tgaacgctta
      961 ctgtttaatt atggcgcaag ttatcgcggt cgaaaaacag caatccctat ctatgaaccg
     1021 gaactgccat tacaggaagg cgctattgct cctatcaatg gtttacagat ggcttcttgt
     1081 attcgcgatg gtagtccgga tgcgtggggc cgtcgcgtta ttatcaatcg cttaacggga
     1141 aagaaaccag atgccgatgg tgttccagaa atctcagaac tgacatatct gttacatagc
     1201 ggtagcgatc gtacgggcgc gttagatttt caggcaagtg ctacagaata tgttccacgc
     1261 cgtgcagctc aggcttcact ggatgaactc atggaagcag ctgccttagt tgaaaaaggt
     1321 gttccgctga cacctgcatt agcacaggcc ctgaatcatg gtacaagtat cggtggcgca
     1381 cgtcctaaag ccctgatcaa tgatgatacg aaaaaattca ttgccaaatt ttcagcgtct
     1441 aatgatacct atagcgttgt gaaagccgag ttcattgcaa tgcgcctggc tagtgcctca
     1501 ggcttagatg tggcgagcgt gagtatcact agggccgccc ataaagatgt tctgctgatc
     1561 gaacgctttg atcgccgtca taccaaagaa ggttggaccc gccaggcgat ggtgagcgca
     1621 ctgaccatcc tgggtctgga tgaaatgatg gcacgctatg cctcttatga ggatcttgcg
     1681 gaactgattc gtcatcgctt tacggctcct aaagatacac tgaaagaact gtatggccgt
     1741 atctgcttta atgttctgtg cggtaatacc gatgatcatg cccgtaatca tgcggccttt
     1801 tgggatggca aaatgatgac gttaaccccg gcatacgata tttgtccaca gtctcgtacg
     1861 ggtacggaag ctacccaggc catgttaatc aaaggtgaag gtcgtgctag taccttagcc
     1921 aattgtctgg cagccgctcc ggattatcat ctgaaagaag cagatgcgac ggcattaatc
     1981 gaacatcaga tcacaacgat tgcggaacag tggcaggctg tgtgtgcaga agcggaatta
     2041 acaccagtgg atcgtcagtt ctttgctggt cgtcagtttc tgaacaacta tgccatcgaa
     2101 ggtttagaag gtcataaagc tctgcatgat gcctttggcg cagcccgcaa agctctgatt
     2161 gctagtggcg atgca
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