##gff-version 3
##sequence-region BBa_K2150126 1 2175
BBa_K2150126	.	promoter	694	747	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2150126	.	non_covalent_binding_site	694	712	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2150126	.	engineered_region	694	747	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2150126	.	promoter	713	718	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2150126	.	promoter	736	741	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2150126	.	non_covalent_binding_site	719	737	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2150126	.	ribosome_entry_site	839	849	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K2150126	.	ribosome_entry_site	839	849	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K2150126	.	ribosome_entry_site	845	848	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K2150126	.	engineered_region	839	849	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K2150126	.	CDS	1	685	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K2150126	.	engineered_tag	661	685	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K2150126	.	CDS	4	620	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K2150126	.	tag	621	654	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K2150126	.	promoter	756	830	.	+	0	ID=BBa_K1679038;Name=BBa_K1679038
BBa_K2150126	.	CDS	856	2175	.	+	0	ID=BBa_K2150121;Name=BBa_K2150121
BBa_K2150126	.	CDS	856	2175	.	+	0	ID=annotation2519784;Name=toxin 4222 CDS;Parent=BBa_K2150121
>BBa_K2150126
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaa
cccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcga
cgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttta
cgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggccta
cagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattata
tgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagg
gaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccag
ccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacga
cgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagag
attgacatccctatcagtgatagagatactgagcactactagagttaaacaaaattatttgtagaggctgtttcgtcct
cacggactcatcagaccggaaagcacatccggtgacagcttactagagtcacacaggactactagatgacgagcaaagc
cgatgcgaccgaagcatttgtgtggatttggttaccgggcgcgaccgaaccagttgttgcgggtcgcctgtttcaggaa
ggtgaacgcttactgtttaattatggcgcaagttatcgcggtcgaaaaacagcaatccctatctatgaaccggaactgc
cattacaggaaggcgctattgctcctatcaatggtttacagatggcttcttgtattcgcgatggtagtccggatgcgtg
gggccgtcgcgttattatcaatcgcttaacgggaaagaaaccagatgccgatggtgttccagaaatctcagaactgaca
tatctgttacatagcggtagcgatcgtacgggcgcgttagattttcaggcaagtgctacagaatatgttccacgccgtg
cagctcaggcttcactggatgaactcatggaagcagctgccttagttgaaaaaggtgttccgctgacacctgcattagc
acaggccctgaatcatggtacaagtatcggtggcgcacgtcctaaagccctgatcaatgatgatacgaaaaaattcatt
gccaaattttcagcgtctaatgatacctatagcgttgtgaaagccgagttcattgcaatgcgcctggctagtgcctcag
gcttagatgtggcgagcgtgagtatcactagggccgcccataaagatgttctgctgatcgaacgctttgatcgccgtca
taccaaagaaggttggacccgccaggcgatggtgagcgcactgaccatcctgggtctggatgaaatgatggcacgctat
gcctcttatgaggatcttgcggaactgattcgtcatcgctttacggctcctaaagatacactgaaagaactgtatggcc
gtatctgctttaatgttctgtgcggtaataccgatgatcatgcccgtaatcatgcggccttttgggatggcaaaatgat
gacgttaaccccggcatacgatatttgtccacagtctcgtacgggtacggaagctacccaggccatgttaatcaaaggt
gaaggtcgtgctagtaccttagccaattgtctggcagccgctccggattatcatctgaaagaagcagatgcgacggcat
taatcgaacatcagatcacaacgattgcggaacagtggcaggctgtgtgtgcagaagcggaattaacaccagtggatcg
tcagttctttgctggtcgtcagtttctgaacaactatgccatcgaaggtttagaaggtcataaagctctgcatgatgcc
tttggcgcagcccgcaaagctctgattgctagtggcgatgca