LOCUS BBa_K2150127 1193 bp DNA linear 25-Nov-2024 DEFINITION Ptet + RIboJ + RBS + toxin 5694 ACCESSION BBa_K2150127 VERSION BBa_K2150127.1 FEATURES Location/Qualifiers CDS 4..620 /label=tetR misc_feature 621..654 /label=SsrA misc_feature 661..685 /label=Help:Barcodes CDS 1..685 /label=BBa_C0040 misc_feature 694..712 /label=TetR 1 misc_feature 694..747 /label=BBa_R0040 promoter 713..718 /label=-35 misc_feature 719..737 /label=TetR 2 promoter 736..741 /label=-10 promoter 694..747 /label=BBa_R0040 promoter 756..830 /label=BBa_K1679038 CDS 837..1193 /label=toxin 5694 CDS CDS 837..1193 /label=BBa_K2150123 ORIGIN 1 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc 61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca 121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta 181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt 241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat 301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta 361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt 421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca 481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa 541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa 601 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa 661 cactgatagt gctagtgtag atcactacta gagtccctat cagtgataga gattgacatc 721 cctatcagtg atagagatac tgagcactac tagagttaaa caaaattatt tgtagaggct 781 gtttcgtcct cacggactca tcagaccgga aagcacatcc ggtgacagct tactagatgc 841 gtatttttaa aacccgttgg tttaatcgcg aagccaaatc tcataccatt aaagatgatg 901 aactgagcga agcaattaat gccgtgttac aaggtaaagc cgataatctg ggcggcggtg 961 tgtataagaa acgcctgaat cagaatcgcg atcgcgcaat tgtgttagcc aaaggtggtg 1021 aacattggtt ttataccttt ctgtatgcca aacaggatat ggcaaatatt agctatcgtg 1081 aactggcagg ctttcgtgaa ctggccaaac attatgcatg tctgaccgaa gatcagatta 1141 ccgcactgat taacaataag gaattagtgg aagttcgtca tgtgtcaaaa aat //