LOCUS       BBa_K2150127            1193 bp    DNA     linear       05-Nov-2024
DEFINITION  Ptet + RIboJ + RBS + toxin 5694
ACCESSION   BBa_K2150127
VERSION     BBa_K2150127.1  
FEATURES             Location/Qualifiers
     CDS             4..620
                     /label=tetR
     misc_feature    621..654
                     /label=SsrA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             1..685
                     /label=BBa_C0040
     misc_feature    694..712
                     /label=TetR 1
     misc_feature    694..747
                     /label=BBa_R0040
     promoter        713..718
                     /label=-35
     misc_feature    719..737
                     /label=TetR 2
     promoter        736..741
                     /label=-10
     promoter        694..747
                     /label=BBa_R0040
     promoter        756..830
                     /label=BBa_K1679038
     CDS             837..1193
                     /label=toxin 5694 CDS
     CDS             837..1193
                     /label=BBa_K2150123
ORIGIN
        1 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
       61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
      541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
      601 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa
      661 cactgatagt gctagtgtag atcactacta gagtccctat cagtgataga gattgacatc
      721 cctatcagtg atagagatac tgagcactac tagagttaaa caaaattatt tgtagaggct
      781 gtttcgtcct cacggactca tcagaccgga aagcacatcc ggtgacagct tactagatgc
      841 gtatttttaa aacccgttgg tttaatcgcg aagccaaatc tcataccatt aaagatgatg
      901 aactgagcga agcaattaat gccgtgttac aaggtaaagc cgataatctg ggcggcggtg
      961 tgtataagaa acgcctgaat cagaatcgcg atcgcgcaat tgtgttagcc aaaggtggtg
     1021 aacattggtt ttataccttt ctgtatgcca aacaggatat ggcaaatatt agctatcgtg
     1081 aactggcagg ctttcgtgaa ctggccaaac attatgcatg tctgaccgaa gatcagatta
     1141 ccgcactgat taacaataag gaattagtgg aagttcgtca tgtgtcaaaa aat
//