BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K2151011
1
BBa_K2151011
p32-B0034-K592009: Blue chromoprotein under control of strong constitutive promoter
2016-08-29T11:00:00Z
2016-10-08T03:29:15Z
.
.
false
false
_2621_
32386
27285
9
false
.
false
Matej Vucak, Katy Baker, James Provan
component2482091
1
BBa_K2151002
component2482093
1
BBa_B0034
component2482095
1
BBa_K592009
annotation2482091
1
BBa_K2151002
range2482091
1
1
50
annotation2482093
1
BBa_B0034
range2482093
1
59
70
annotation2482095
1
BBa_K592009
range2482095
1
77
745
BBa_K2151002
1
BBa_K2151002
p32, strong constitutive <i>S. thermophilus</i> promoter
2016-08-29T11:00:00Z
2016-10-08T02:59:30Z
.
S. thermophilus promoter
false
false
_2621_
32386
27285
9
false
.
false
Matej Vucak, Katy Baker, James Provan
annotation2482028
1
-35 site
range2482028
1
1
6
annotation2482029
1
-10 site
range2482029
1
24
29
BBa_K592009
1
amilCP
amilCP, blue chromoprotein
2011-09-17T11:00:00Z
2015-05-08T01:12:48Z
Acropora millepora
Released HQ 2013
This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
false
false
_763_
0
7929
9
In stock
true
Illegal internal restriction site had to be removed (EcoRI).
false
Lei Sun
annotation2131628
1
amilCP
range2131628
1
1
666
BBa_K2151011_sequence
1
gtagaaaaaaacttcacaaaatgctatactaggtaggtaaaaaaatattctactagagaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2151002_sequence
1
tagaaaaaaacttcacaaaatgctatactaggtaggtaaaaaaatattc
BBa_K592009_sequence
1
atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z