LOCUS BBa_K215105 4633 bp DNA linear 22-Nov-2024 DEFINITION prtD_prtE_prtF_BBa_B0014 ACCESSION BBa_K215105 VERSION BBa_K215105.1 FEATURES Location/Qualifiers RBS 3..18 /label=native RBS CDS 19..1749 /label=prtD CDS 1..1749 /label=BBa_K215100 RBS 1760..1772 /label=native rbs CDS 1773..3119 /label=prtE CDS 1758..3119 /label=BBa_K215101 RBS 3130..3141 /label=Native RBS CDS 3142..4530 /label=prtF CDS 3128..4530 /label=BBa_K215102 stem_loop 4546..4565 /label=T7 TE misc_feature 4572..4572 /label=stop polyA_site 4566..4579 /label=polya misc_feature 4539..4579 /label=BBa_B0012 terminator 4539..4579 /label=BBa_B0012 stem_loop 4600..4622 /label=stem_loop misc_feature 4588..4633 /label=BBa_B0011 terminator 4588..4633 /label=BBa_B0011 ORIGIN 1 aggggagaga gccctgccat gaacgcgtct agcgaacgtg accgttctct gtttggtgtg 61 ctgcgtcagt tccgccgctc cttttggtct gttggcatct tcagcgcggt catcaatgtt 121 ctgatgctgg cacctagcgt ctacatgctc caggtttacg accgtgtcct cgccagcggc 181 aacggtatta ctctgctgat gctcacgctc ctgatggcgg gtctgtgcgc cttcatgggt 241 gctctcgaat gggttcgcag cctgctggta gttcgtctcg gtactcgcat cgacctcgcg 301 ctcaatcaag acgtttttaa cgcggctttc gcgcgtaatc tggaagctgg tgatggccgt 361 gccggtctgg cgctgaccga cctcaccctg ctccgtcaat tcatcacggg taatgcactg 421 ttcgcgttct tcgacgttcc gtggttcccg ctctttctcc tcgtactgtt cctgctgcac 481 ccttggctcg gcatgctcgc actgggtggt acggttgtac ctggtggcgt aggcctcgca 541 gaaccggcgt ctgaccagtc taccgcgggt ggctctaatc aacaaagcca acaagccact 601 catctcgcgg atgcgcagct ccgtaatgcg gacgttatcg aagcgatggg tatgctcggt 661 aacctccgtc gtcgctggct ggcgcgtcac taccgcttta tctccctcca aaatctcgct 721 tccgagcgcg cagccgccgt tggtggtgcg tccaaatact ctcgtattgc actccaaagc 781 ctcatgctgg gcctcggtgc gctcctcgcg atcgacggca aaatcacgcc gggtatgatg 841 attgcgggct ctatcctcgt cggtcgtgtt ctgtccccga ttgaccagct gatcggtgtt 901 tggaaacagt ggtcttctgc ccgcattgcg tggcagcgcc tgacccgtct gattgccgca 961 tatccgccac gccctgcggc tatggctctc ccagcgccgg aaggccacct gtccgttgag 1021 caagtttctc tgcgtaccgc acaaggcaat actcgcctgc aaaacatcca tttctccctg 1081 caagcgggtg agaccctggt tatcctgggc gcctctggtt ctggcaaatc ttctctcgct 1141 cgtctgctcg tgggtgcaca atcccctact caaggtaaag ttcgcctcga tggtgcggac 1201 ctgaaccaag ttgacaaaaa caccttcggt ccgaccattg gttacctccc gcaggacgtt 1261 caactgttca aaggtagcct ggcggaaaac attgcccgtt tcggcgacgc cgacccggaa 1321 aaggttgttg cggcagctaa gctcgccggt gttcacgaac tcatcctgtc tctgcctaat 1381 ggttacgaca ctgagctggg tgacggcggt ggcggcctgt ctggtggtca acgtcagcgt 1441 atcggtctcg cacgcgcaat gtacggcgac ccgtgtctcc tcattctgga cgaaccgaac 1501 gcatctctgg actctgaggg tgaccaagca ctcatgcagg ctattgtggc gctccagaaa 1561 cgtggtgcaa ccgttgtact cattacgcac cgtccggcac tcaccaccct cgctcagaaa 1621 atcctgatcc tccacgaggg tcaacagcaa cgtatgggcc tggcccgtga cgttctgacg 1681 gaactccagc agcgctccgc ggcaaaccag gcgcgtatga acccgaccgc agcaatgcca 1741 cagtaataat actagagagc agggatctcg tgatgactgg tatggatatt accactcagg 1801 acgaactcaa cgaagctgcc atgcgtgacc gtgcgagccg tgacgaagaa cgtgcgctcc 1861 gtctgggttg gtggctcgtc ctcgcgggtt tcggtggttt cctcctgtgg gcgctcctgg 1921 ctccactgga caagggtgtt gcggttcagg gtaacgttgt agtttccggt aaccgtaaag 1981 ttatccagca catgcaaggc ggtattgttg accgcatcca agttaaggac ggcgaccgtg 2041 ttgccgctgg tcaggttctg ctcactctca atgcagtcga cgctcgtacc acctccgagg 2101 gcctgggttc tcagtacgat cagctcatcg cgcgtgaagc gcgcctcctc gccgagcagc 2161 gtaaccagtc ttctctcgct gcaacgccac gcctgacgca ggcacgccag cgtccggaaa 2221 tggcggcgat tatcgccctc caggaagacc tgctgcgctc tcgtcagcag tctctcaaac 2281 tggaaattga cggtgtccgt gcatctatcg atggtctgga aacttctctg ggtgctctgc 2341 aaaaagtgat gtcttctaaa cagtctgaac aagcgactct gtctcaacag ctgcaaggtc 2401 tgcgtccgct ggccgcagac aactacgttc cgcgtaacaa gatgctcgaa accgaacgtc 2461 tcttcgccca ggtttctggc gagctggccc agacctctgg tgaagttggt cgtacccgtc 2521 gtgacattca acaacagaaa ctccgcattg cgcagcgcca gcaggagtac gacaaagaag 2581 tcaactctga actgtctgac gttcaggcca aactgaacga agtaatctct cagcgtgaaa 2641 aggcggactt caatctcgct aacgtgcagg ttcgtgcgcc ggtggcgggc actgtggtgg 2701 atatgaaaat cttcaccgaa ggtggcgtaa ttgctccggg tcaagttatg atggacattg 2761 ttccggagga tcagccactg ctggtagatg gccgtatccc ggttgagatg gttgacaaag 2821 tatggagcgg tctgccggtc gaactgcaat ttactgcgtt ttcccaatct actacgccgc 2881 gtgtccctgg tactgtaacc ctgctgagcg cggatcgcct ggtggacgaa aaagatggca 2941 ccccttacta tggcctccgt attcaggtgt ctgaggaagg taaacgtagc ctccacggcc 3001 tggagattaa accgggtatg cctgtccaag gttttgtgcg tacgggcgaa cgctctttca 3061 tcaactacct cttcaaaccg ctcatggacc gtatgcacct cgcactgacc gaagaataat 3121 actagagagc ggaaggaagc aatgcgccgt aaagcagttc tgctcactgt ggttctgagc 3181 ctgtctggtg gtagcgccca agcgatgggt ctgctggacg cgtgggaact cgcgctccgc 3241 aatgatgcgc aactgcgtgc ggcaggtttc gaacgtgacg cgggtcaaga ggaagttgcg 3301 attggtcgcg caggcctcct gccgagcctg caatacacgt acggtgcgaa ctactcccac 3361 tccaaagtta cccagcgcga tcgtaccctg aacaacacca cgaaacgtga ttatgataac 3421 tacgtttcta cgctgaccct gcgtcagcca ctcctggact acgcggcatg ggcgcgttat 3481 cagcaaggcg taactcgtaa actgatggcg gaccagcgtt ttcgcgaccg ttcccaggat 3541 ctgatggttc gtctgtacca gtcctggagc gaagccctgc tggcgcagga aaagctgatg 3601 ctcctcgacg ctcagcgtcg tgcataccag gaacagctcg cactgaatcg ccgtctcctg 3661 gctgccggtg agggcacgca aaccgacctg cgtgagaccg aagcacgtta taccgtcacc 3721 gaggcgcagc gtatcgaaca ggaggacacc ctcgatgccg ccatgactga cctggaaaac 3781 atgatgggct ctcctctcca gatccaagac ctgtctccac tggctctcga cactctcccg 3841 gataacgtaa cggaaaaccg tagcctctct cagtggcgtg aactcacggt acgtcacaac 3901 gccaaactgg cagtacaacg tgagaacgtg gattactctc gctacgaaat cgagcgcaac 3961 cgtgcgggcc acctcccgac cctggacctg gttgcctcta cccgtaattc cctctccgag 4021 agcgaataca actacaatca aaaatacgac acccagaccg taggtctgca agttcgtgtt 4081 cctctctact ctggcggtgc ggtttctgcg tctatgcgcc aagcggcagc tgagtatcaa 4141 cagtcccagg cggaactgga caatcagact cgtcagacct tcgcggagct gcgtcgccag 4201 ttcaacctgt gcgcaaacgg tgctgcgaaa atccgtgctt ggcagatgtc tgtagctgct 4261 gccgaagagg ccattcgtgc gacccgtcag tctgttgcgg gtggtgaacg tattaacctg 4321 gatgtgctca tggctgagca ggaatggtat aatgcccgtc gtgagctgac ggaggttaag 4381 taccgttggc tgcaagcgtg gctcaatctg cgctacacgg ctggtactct caacgaacaa 4441 gatatgatgc agctggcagc ctggttccag tctgcgccgg ttatcaataa aaccggcatc 4501 aatgctgcca cgggcaacaa gaccaattaa tactagagtc acactggctc accttcgggt 4561 gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc 4621 ttttttatta ttt //