LOCUS BBa_K215107 4676 bp DNA linear 25-Nov-2024 DEFINITION Extracellular Secretion System ACCESSION BBa_K215107 VERSION BBa_K215107.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 46..61 /label=native RBS CDS 62..1792 /label=prtD CDS 44..1792 /label=BBa_K215100 RBS 1803..1815 /label=native rbs CDS 1816..3162 /label=prtE CDS 1801..3162 /label=BBa_K215101 RBS 3173..3184 /label=Native RBS CDS 3185..4573 /label=prtF CDS 3171..4573 /label=BBa_K215102 stem_loop 4589..4608 /label=T7 TE misc_feature 4615..4615 /label=stop polyA_site 4609..4622 /label=polya misc_feature 4582..4622 /label=BBa_B0012 terminator 4582..4622 /label=BBa_B0012 stem_loop 4643..4665 /label=stem_loop misc_feature 4631..4676 /label=BBa_B0011 terminator 4631..4676 /label=BBa_B0011 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaggggag agagccctgc 61 catgaacgcg tctagcgaac gtgaccgttc tctgtttggt gtgctgcgtc agttccgccg 121 ctccttttgg tctgttggca tcttcagcgc ggtcatcaat gttctgatgc tggcacctag 181 cgtctacatg ctccaggttt acgaccgtgt cctcgccagc ggcaacggta ttactctgct 241 gatgctcacg ctcctgatgg cgggtctgtg cgccttcatg ggtgctctcg aatgggttcg 301 cagcctgctg gtagttcgtc tcggtactcg catcgacctc gcgctcaatc aagacgtttt 361 taacgcggct ttcgcgcgta atctggaagc tggtgatggc cgtgccggtc tggcgctgac 421 cgacctcacc ctgctccgtc aattcatcac gggtaatgca ctgttcgcgt tcttcgacgt 481 tccgtggttc ccgctctttc tcctcgtact gttcctgctg cacccttggc tcggcatgct 541 cgcactgggt ggtacggttg tacctggtgg cgtaggcctc gcagaaccgg cgtctgacca 601 gtctaccgcg ggtggctcta atcaacaaag ccaacaagcc actcatctcg cggatgcgca 661 gctccgtaat gcggacgtta tcgaagcgat gggtatgctc ggtaacctcc gtcgtcgctg 721 gctggcgcgt cactaccgct ttatctccct ccaaaatctc gcttccgagc gcgcagccgc 781 cgttggtggt gcgtccaaat actctcgtat tgcactccaa agcctcatgc tgggcctcgg 841 tgcgctcctc gcgatcgacg gcaaaatcac gccgggtatg atgattgcgg gctctatcct 901 cgtcggtcgt gttctgtccc cgattgacca gctgatcggt gtttggaaac agtggtcttc 961 tgcccgcatt gcgtggcagc gcctgacccg tctgattgcc gcatatccgc cacgccctgc 1021 ggctatggct ctcccagcgc cggaaggcca cctgtccgtt gagcaagttt ctctgcgtac 1081 cgcacaaggc aatactcgcc tgcaaaacat ccatttctcc ctgcaagcgg gtgagaccct 1141 ggttatcctg ggcgcctctg gttctggcaa atcttctctc gctcgtctgc tcgtgggtgc 1201 acaatcccct actcaaggta aagttcgcct cgatggtgcg gacctgaacc aagttgacaa 1261 aaacaccttc ggtccgacca ttggttacct cccgcaggac gttcaactgt tcaaaggtag 1321 cctggcggaa aacattgccc gtttcggcga cgccgacccg gaaaaggttg ttgcggcagc 1381 taagctcgcc ggtgttcacg aactcatcct gtctctgcct aatggttacg acactgagct 1441 gggtgacggc ggtggcggcc tgtctggtgg tcaacgtcag cgtatcggtc tcgcacgcgc 1501 aatgtacggc gacccgtgtc tcctcattct ggacgaaccg aacgcatctc tggactctga 1561 gggtgaccaa gcactcatgc aggctattgt ggcgctccag aaacgtggtg caaccgttgt 1621 actcattacg caccgtccgg cactcaccac cctcgctcag aaaatcctga tcctccacga 1681 gggtcaacag caacgtatgg gcctggcccg tgacgttctg acggaactcc agcagcgctc 1741 cgcggcaaac caggcgcgta tgaacccgac cgcagcaatg ccacagtaat aatactagag 1801 agcagggatc tcgtgatgac tggtatggat attaccactc aggacgaact caacgaagct 1861 gccatgcgtg accgtgcgag ccgtgacgaa gaacgtgcgc tccgtctggg ttggtggctc 1921 gtcctcgcgg gtttcggtgg tttcctcctg tgggcgctcc tggctccact ggacaagggt 1981 gttgcggttc agggtaacgt tgtagtttcc ggtaaccgta aagttatcca gcacatgcaa 2041 ggcggtattg ttgaccgcat ccaagttaag gacggcgacc gtgttgccgc tggtcaggtt 2101 ctgctcactc tcaatgcagt cgacgctcgt accacctccg agggcctggg ttctcagtac 2161 gatcagctca tcgcgcgtga agcgcgcctc ctcgccgagc agcgtaacca gtcttctctc 2221 gctgcaacgc cacgcctgac gcaggcacgc cagcgtccgg aaatggcggc gattatcgcc 2281 ctccaggaag acctgctgcg ctctcgtcag cagtctctca aactggaaat tgacggtgtc 2341 cgtgcatcta tcgatggtct ggaaacttct ctgggtgctc tgcaaaaagt gatgtcttct 2401 aaacagtctg aacaagcgac tctgtctcaa cagctgcaag gtctgcgtcc gctggccgca 2461 gacaactacg ttccgcgtaa caagatgctc gaaaccgaac gtctcttcgc ccaggtttct 2521 ggcgagctgg cccagacctc tggtgaagtt ggtcgtaccc gtcgtgacat tcaacaacag 2581 aaactccgca ttgcgcagcg ccagcaggag tacgacaaag aagtcaactc tgaactgtct 2641 gacgttcagg ccaaactgaa cgaagtaatc tctcagcgtg aaaaggcgga cttcaatctc 2701 gctaacgtgc aggttcgtgc gccggtggcg ggcactgtgg tggatatgaa aatcttcacc 2761 gaaggtggcg taattgctcc gggtcaagtt atgatggaca ttgttccgga ggatcagcca 2821 ctgctggtag atggccgtat cccggttgag atggttgaca aagtatggag cggtctgccg 2881 gtcgaactgc aatttactgc gttttcccaa tctactacgc cgcgtgtccc tggtactgta 2941 accctgctga gcgcggatcg cctggtggac gaaaaagatg gcacccctta ctatggcctc 3001 cgtattcagg tgtctgagga aggtaaacgt agcctccacg gcctggagat taaaccgggt 3061 atgcctgtcc aaggttttgt gcgtacgggc gaacgctctt tcatcaacta cctcttcaaa 3121 ccgctcatgg accgtatgca cctcgcactg accgaagaat aatactagag agcggaagga 3181 agcaatgcgc cgtaaagcag ttctgctcac tgtggttctg agcctgtctg gtggtagcgc 3241 ccaagcgatg ggtctgctgg acgcgtggga actcgcgctc cgcaatgatg cgcaactgcg 3301 tgcggcaggt ttcgaacgtg acgcgggtca agaggaagtt gcgattggtc gcgcaggcct 3361 cctgccgagc ctgcaataca cgtacggtgc gaactactcc cactccaaag ttacccagcg 3421 cgatcgtacc ctgaacaaca ccacgaaacg tgattatgat aactacgttt ctacgctgac 3481 cctgcgtcag ccactcctgg actacgcggc atgggcgcgt tatcagcaag gcgtaactcg 3541 taaactgatg gcggaccagc gttttcgcga ccgttcccag gatctgatgg ttcgtctgta 3601 ccagtcctgg agcgaagccc tgctggcgca ggaaaagctg atgctcctcg acgctcagcg 3661 tcgtgcatac caggaacagc tcgcactgaa tcgccgtctc ctggctgccg gtgagggcac 3721 gcaaaccgac ctgcgtgaga ccgaagcacg ttataccgtc accgaggcgc agcgtatcga 3781 acaggaggac accctcgatg ccgccatgac tgacctggaa aacatgatgg gctctcctct 3841 ccagatccaa gacctgtctc cactggctct cgacactctc ccggataacg taacggaaaa 3901 ccgtagcctc tctcagtggc gtgaactcac ggtacgtcac aacgccaaac tggcagtaca 3961 acgtgagaac gtggattact ctcgctacga aatcgagcgc aaccgtgcgg gccacctccc 4021 gaccctggac ctggttgcct ctacccgtaa ttccctctcc gagagcgaat acaactacaa 4081 tcaaaaatac gacacccaga ccgtaggtct gcaagttcgt gttcctctct actctggcgg 4141 tgcggtttct gcgtctatgc gccaagcggc agctgagtat caacagtccc aggcggaact 4201 ggacaatcag actcgtcaga ccttcgcgga gctgcgtcgc cagttcaacc tgtgcgcaaa 4261 cggtgctgcg aaaatccgtg cttggcagat gtctgtagct gctgccgaag aggccattcg 4321 tgcgacccgt cagtctgttg cgggtggtga acgtattaac ctggatgtgc tcatggctga 4381 gcaggaatgg tataatgccc gtcgtgagct gacggaggtt aagtaccgtt ggctgcaagc 4441 gtggctcaat ctgcgctaca cggctggtac tctcaacgaa caagatatga tgcagctggc 4501 agcctggttc cagtctgcgc cggttatcaa taaaaccggc atcaatgctg ccacgggcaa 4561 caagaccaat taatactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 4621 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttattt //