BBa_K215100 1 BBa_K215100 prtD from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence. false Josef Dunbar annotation2027446 1 prtD range2027446 1 19 1749 annotation2027445 1 native RBS range2027445 1 3 18 BBa_K215101 1 BBa_K215101 prtE from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and rhibosome binding site. false Josef Dunbar annotation2027448 1 prtE range2027448 1 16 1362 annotation2027447 1 native rbs range2027447 1 3 15 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_K215108 1 BBa_K215108 Extracellular Secretion System Medium Promoter 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23114, and has the translational terminator BBa_B0014. In PSB3T5 false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site. false Josef Dunbar component2027576 1 BBa_K215101 component2027573 1 BBa_K215100 component2027584 1 BBa_B0011 component2027579 1 BBa_K215102 component2027580 1 BBa_B0012 component2027570 1 BBa_J23114 annotation2027570 1 BBa_J23114 range2027570 1 1 35 annotation2027576 1 BBa_K215101 range2027576 1 1801 3162 annotation2027580 1 BBa_B0012 range2027580 1 4582 4622 annotation2027584 1 BBa_B0011 range2027584 1 4631 4676 annotation2027579 1 BBa_K215102 range2027579 1 3171 4573 annotation2027573 1 BBa_K215100 range2027573 1 44 1792 BBa_K215102 1 BBa_K215102 prtF from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site. false Josef Dunbar annotation2027450 1 prtF range2027450 1 15 1403 annotation2027449 1 Native RBS range2027449 1 3 14 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_J23114 1 BBa_J23114 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K215102_sequence 1 agcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaa BBa_K215108_sequence 1 tttatggctagctcagtcctaggtacaatgctagctactagagaggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_J23114_sequence 1 tttatggctagctcagtcctaggtacaatgctagc BBa_K215101_sequence 1 agcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataa BBa_K215100_sequence 1 aggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z