##gff-version 3
##sequence-region BBa_K215109 1 4676
BBa_K215109	.	CDS	44	1792	.	+	0	ID=BBa_K215100;Name=BBa_K215100
BBa_K215109	.	CDS	62	1792	.	+	0	ID=annotation2027446;Name=prtD;Parent=BBa_K215100
BBa_K215109	.	ribosome_entry_site	46	61	.	+	0	ID=annotation2027445;Name=native RBS;Parent=BBa_K215100
BBa_K215109	.	promoter	1	35	.	+	0	ID=BBa_J23113;Name=BBa_J23113
BBa_K215109	.	CDS	1801	3162	.	+	0	ID=BBa_K215101;Name=BBa_K215101
BBa_K215109	.	CDS	1816	3162	.	+	0	ID=annotation2027448;Name=prtE;Parent=BBa_K215101
BBa_K215109	.	ribosome_entry_site	1803	1815	.	+	0	ID=annotation2027447;Name=native rbs;Parent=BBa_K215101
BBa_K215109	.	CDS	3171	4573	.	+	0	ID=BBa_K215102;Name=BBa_K215102
BBa_K215109	.	CDS	3185	4573	.	+	0	ID=annotation2027450;Name=prtF;Parent=BBa_K215102
BBa_K215109	.	ribosome_entry_site	3173	3184	.	+	0	ID=annotation2027449;Name=Native RBS;Parent=BBa_K215102
BBa_K215109	.	terminator	4631	4676	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_K215109	.	stem_loop	4643	4665	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K215109	.	engineered_region	4631	4676	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K215109	.	terminator	4582	4622	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K215109	.	engineered_region	4582	4622	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K215109	.	stop_codon	4615	4615	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K215109	.	stem_loop	4589	4608	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K215109	.	polyA_site	4609	4622	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K215109
ctgatggctagctcagtcctagggattatgctagctactagagaggggagagagccctgccatgaacgcgtctagcgaa
cgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatca
atgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactct
gctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagtt
cgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtg
atggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttctt
cgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggt
acggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagcc
aacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccg
tcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggt
gcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatca
cgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaa
acagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatg
gctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaa
acatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcg
tctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaac
accttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtt
tcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaa
tggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgca
atgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgc
aggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctca
gaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgc
tccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgat
gactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgt
gcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggaca
agggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttga
ccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtacc
acctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagt
cttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaaga
cctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttct
ctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtc
cgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagct
ggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcag
gagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaagg
cggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaagg
tggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccg
gttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgc
gtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccg
tattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtg
cgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaat
aatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcg
cccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacg
tgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactac
tcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgc
tgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcgga
ccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaa
aagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtg
agggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacac
cctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctc
gacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaac
tggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctgga
cctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggt
ctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagt
cccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgc
tgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggt
ggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagt
accgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagc
ctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaatactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccg
gcttttttattattt