BBa_K215102 1 BBa_K215102 prtF from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site. false Josef Dunbar annotation2027450 1 prtF range2027450 1 15 1403 annotation2027449 1 Native RBS range2027449 1 3 14 BBa_K215100 1 BBa_K215100 prtD from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence. false Josef Dunbar annotation2027445 1 native RBS range2027445 1 3 18 annotation2027446 1 prtD range2027446 1 19 1749 BBa_K215101 1 BBa_K215101 prtE from erwinia chrysanthemi 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is one of the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and rhibosome binding site. false Josef Dunbar annotation2027448 1 prtE range2027448 1 16 1362 annotation2027447 1 native rbs range2027447 1 3 15 BBa_J23113 1 BBa_J23113 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K215109 1 BBa_K215109 Extracellular Secretion System Low Promoter 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Gene Bank ID: Erwinia chrysanthemi M60395.1 This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a weak constitutive promoter, BBa_J23113, and has the translational terminator BBa_B0014. In PSB3T5 false false _320_ 0 5596 9 It's complicated false Synthesized to be bio-bricked, keeps original protein sequence and ribosome binding site. false Josef Dunbar component2027610 1 BBa_K215102 component2027611 1 BBa_B0012 component2027607 1 BBa_K215101 component2027615 1 BBa_B0011 component2027604 1 BBa_K215100 component2027601 1 BBa_J23113 annotation2027604 1 BBa_K215100 range2027604 1 44 1792 annotation2027601 1 BBa_J23113 range2027601 1 1 35 annotation2027607 1 BBa_K215101 range2027607 1 1801 3162 annotation2027610 1 BBa_K215102 range2027610 1 3171 4573 annotation2027615 1 BBa_B0011 range2027615 1 4631 4676 annotation2027611 1 BBa_B0012 range2027611 1 4582 4622 BBa_K215102_sequence 1 agcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaa BBa_K215101_sequence 1 agcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataa BBa_J23113_sequence 1 ctgatggctagctcagtcctagggattatgctagc BBa_K215100_sequence 1 aggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K215109_sequence 1 ctgatggctagctcagtcctagggattatgctagctactagagaggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccgccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccaggtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgccttcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaagacgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctgctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgttcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggcgtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaatgcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccctccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcatgctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggtcgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctgacccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagcaagtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggttatcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaagttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgttcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagctaagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcctgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaaccgaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgttgtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgtatgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagcaatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacgaagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggtttcggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggtaaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctggtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcatcgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgccagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaattgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtctgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatgctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtgacattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgttcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccggtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattgttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggtcgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgcctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacggcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaaaccgctcatggaccgtatgcacctcgcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagcagttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcgctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcaggcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaacaacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgggcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttcgtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcataccaggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgttataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgggctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcctctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctacgaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcgaatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggtttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttcgcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctgccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagcaggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacggctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggcatcaatgctgccacgggcaacaagaccaattaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z