##gff-version 3
##sequence-region BBa_K2151201 1 4598
BBa_K2151201	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K2151201	.	engineered_region	44	4598	.	+	0	ID=BBa_K2151200;Name=BBa_K2151200
BBa_K2151201	.	engineered_region	44	965	.	+	0	ID=BBa_K118014;Name=BBa_K118014;Parent=BBa_K2151200
BBa_K2151201	.	CDS	54	965	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K118014
BBa_K2151201	.	ribosome_entry_site	44	47	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K118014
BBa_K2151201	.	engineered_region	3437	4598	.	+	0	ID=BBa_K118013;Name=BBa_K118013;Parent=BBa_K2151200
BBa_K2151201	.	ribosome_entry_site	3437	3440	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K118013
BBa_K2151201	.	CDS	3447	4598	.	+	0	ID=BBa_K118008;Name=BBa_K118008;Parent=BBa_K118013
BBa_K2151201	.	CDS	3447	4595	.	+	0	ID=annotation1995716;Name=crtY coding sequence;Parent=BBa_K118008
BBa_K2151201	.	engineered_region	974	3428	.	+	0	ID=BBa_K118009;Name=BBa_K118009;Parent=BBa_K2151200
BBa_K2151201	.	ribosome_entry_site	1931	1940	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K118009
BBa_K2151201	.	sequence_feature	1931	1933	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K2151201	.	ribosome_entry_site	1934	1940	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K2151201	.	CDS	990	1922	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K118009
BBa_K2151201	.	CDS	990	1919	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K2151201	.	CDS	1947	3428	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K118009
BBa_K2151201	.	point_mutation	2835	2840	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K2151201	.	CDS	1947	3425	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K2151201	.	point_mutation	2424	2429	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K2151201	.	ribosome_entry_site	974	983	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K118009
BBa_K2151201	.	sequence_feature	974	976	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K2151201	.	ribosome_entry_site	977	983	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
>BBa_K2151201
tttacggctagctcagtcctaggtatagtgctagctactagaggaggtactagatgacggtctgcgcaaaaaaacacgt
tcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggaggga
gaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgc
tgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgc
ggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcat
tacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcc
tcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagtt
caaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctg
ttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcac
ttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcagga
cgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgcc
agtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcg
ctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaac
gatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctc
tacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaa
cgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtt
tgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatg
gatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttga
tgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgac
caatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggt
ctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcag
aaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagca
ggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgccc
gaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgc
atctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcagg
cttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaaccc
ggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgcca
ttgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcct
gtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccc
cgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactg
tcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacag
taaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatccc
ttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccg
gcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatat
ggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaat
gcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaacta
agcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttg
tttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctg
cacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatt
taggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcatta
catgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctat
catggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccatta
ctaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagc
aggtttgatgctggaggatctgatataataatactagaggaggtactagatgcaaccgcattatgatctgattctcgtg
ggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacg
ccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagc
tccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttt
tgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcag
aggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgc
ggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcg
tctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgacca
gattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgacta
tgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaat
gccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctatt
cactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccat
tacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggaccc
gccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaac
tcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgac
gactcatcgttaataa