BBa_K2151201
type: ComponentDefinition
persistent identity: BBa K2151201
display id: BBa_K2151201
version: 1
was generated by: iGEM to SBOL conversion
description: J23106-crtEBIY
created: 2016-08-29T11:00:00Z
modified: 2016-08-30T09:11:57Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2621_
m user id: 27285
owner id: 27285
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Pinja Hartikainen, Liam Pollock, James Provan
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2151200
component: BBa_J23106
sequence annotation: BBa_J23106
sequence annotation: BBa_K2151200
sequence: https://synbiohub.org/public/igem/BBa_K2151201_sequence/1

BBa_K2151200
type: Component
persistent identity: component2482142
display id: component2482142
version: 1
top level: BBa_K2151201
definition: BBa_K2151200
access: public

BBa_J23106
type: Component
persistent identity: component2482124
display id: component2482124
version: 1
top level: BBa_K2151201
definition: BBa_J23106
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2482124
display id: annotation2482124
version: 1
top level: BBa_K2151201
location: https://synbiohub.org/public/igem/BBa_K2151201/annotation2482124/range2482124/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K2151201/annotation2482124/range2482124/1
type: Range
persistent identity: range2482124
display id: range2482124
version: 1
top level: BBa_K2151201
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K2151200
type: SequenceAnnotation
persistent identity: annotation2482142
display id: annotation2482142
version: 1
top level: BBa_K2151201
location: https://synbiohub.org/public/igem/BBa_K2151201/annotation2482142/range2482142/1
component: BBa_K2151200

https://synbiohub.org/public/igem/BBa_K2151201/annotation2482142/range2482142/1
type: Range
persistent identity: range2482142
display id: range2482142
version: 1
top level: BBa_K2151201
direction: direction/reverse
start: 44
end: 4598
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K2151200
type: ComponentDefinition
persistent identity: BBa K2151200
display id: BBa_K2151200
version: 1
was generated by: iGEM to SBOL conversion
description: crtEBIY
created: 2016-08-29T11:00:00Z
modified: 2016-08-30T09:08:47Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2621_
m user id: 27285
owner id: 27285
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Pinja Hartikainen, Liam Pollock, James Provan
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K118009
component: BBa_K118014
component: BBa_K118013
sequence annotation: BBa_K118014
sequence annotation: BBa_K118009
sequence annotation: BBa_K118013
sequence: https://synbiohub.org/public/igem/BBa_K2151200_sequence/1

BBa_K118009
type: Component
persistent identity: component2482120
display id: component2482120
version: 1
top level: BBa_K2151200
definition: BBa_K118009
access: public

BBa_K118014
type: Component
persistent identity: component2482109
display id: component2482109
version: 1
top level: BBa_K2151200
definition: BBa_K118014
access: public

BBa_K118013
type: Component
persistent identity: component2482123
display id: component2482123
version: 1
top level: BBa_K2151200
definition: BBa_K118013
access: public

BBa_K118014
type: SequenceAnnotation
persistent identity: annotation2482109
display id: annotation2482109
version: 1
top level: BBa_K2151200
location: https://synbiohub.org/public/igem/BBa_K2151200/annotation2482109/range2482109/1
component: BBa_K118014

https://synbiohub.org/public/igem/BBa_K2151200/annotation2482109/range2482109/1
type: Range
persistent identity: range2482109
display id: range2482109
version: 1
top level: BBa_K2151200
direction: direction/reverse
start: 1
end: 922
orientation: inline

BBa_K118009
type: SequenceAnnotation
persistent identity: annotation2482120
display id: annotation2482120
version: 1
top level: BBa_K2151200
location: https://synbiohub.org/public/igem/BBa_K2151200/annotation2482120/range2482120/1
component: BBa_K118009

https://synbiohub.org/public/igem/BBa_K2151200/annotation2482120/range2482120/1
type: Range
persistent identity: range2482120
display id: range2482120
version: 1
top level: BBa_K2151200
direction: direction/reverse
start: 931
end: 3385
orientation: inline

BBa_K118013
type: SequenceAnnotation
persistent identity: annotation2482123
display id: annotation2482123
version: 1
top level: BBa_K2151200
location: https://synbiohub.org/public/igem/BBa_K2151200/annotation2482123/range2482123/1
component: BBa_K118013

https://synbiohub.org/public/igem/BBa_K2151200/annotation2482123/range2482123/1
type: Range
persistent identity: range2482123
display id: range2482123
version: 1
top level: BBa_K2151200
direction: direction/reverse
start: 3394
end: 4555
orientation: inline

BBa_K118009
type: ComponentDefinition
persistent identity: BBa K118009
display id: BBa_K118009
version: 1
was generated by: iGEM to SBOL conversion
description: crtB (phytoene synthase) + crtI (phytoene dehydrogenase)
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
mutable description: crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. crtI from P. ananatis encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts phytoene to lycopene.
discontinued: false
dominant: false
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No special considerations
star: false
creator: Andrew Hall
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K118002
component: BBa_J15001
component: BBa_K118003
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K118009_sequence/1

BBa_K118002
type: Component
persistent identity: component1972406
display id: component1972406
version: 1
top level: BBa_K118009
definition: BBa_K118002
access: public

BBa_J15001
type: Component
persistent identity: component1972405
display id: component1972405
version: 1
top level: BBa_K118009
definition: BBa_J15001
access: public

BBa_K118003
type: Component
persistent identity: component1972412
display id: component1972412
version: 1
top level: BBa_K118009
definition: BBa_K118003
access: public

BBa_J15001
type: Component
persistent identity: component1972409
display id: component1972409
version: 1
top level: BBa_K118009
definition: BBa_J15001
access: public

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1972405
display id: annotation1972405
version: 1
top level: BBa_K118009
location: https://synbiohub.org/public/igem/BBa_K118009/annotation1972405/range1972405/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118009/annotation1972405/range1972405/1
type: Range
persistent identity: range1972405
display id: range1972405
version: 1
top level: BBa_K118009
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation1972406
display id: annotation1972406
version: 1
top level: BBa_K118009
location: https://synbiohub.org/public/igem/BBa_K118009/annotation1972406/range1972406/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K118009/annotation1972406/range1972406/1
type: Range
persistent identity: range1972406
display id: range1972406
version: 1
top level: BBa_K118009
direction: direction/reverse
start: 17
end: 949
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1972409
display id: annotation1972409
version: 1
top level: BBa_K118009
location: https://synbiohub.org/public/igem/BBa_K118009/annotation1972409/range1972409/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118009/annotation1972409/range1972409/1
type: Range
persistent identity: range1972409
display id: range1972409
version: 1
top level: BBa_K118009
direction: direction/reverse
start: 958
end: 967
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation1972412
display id: annotation1972412
version: 1
top level: BBa_K118009
location: https://synbiohub.org/public/igem/BBa_K118009/annotation1972412/range1972412/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K118009/annotation1972412/range1972412/1
type: Range
persistent identity: range1972412
display id: range1972412
version: 1
top level: BBa_K118009
direction: direction/reverse
start: 974
end: 2455
orientation: inline

BBa_K118013
type: ComponentDefinition
persistent identity: BBa K118013
display id: BBa_K118013
version: 1
was generated by: iGEM to SBOL conversion
description: rbs+crtY (lycopene B-cyclase)
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: The synthetic ribosome binding site was added by fusion PCR to the ''crtY'' gene, which was amplified from ''P. ananatis'' genomic DNA.
owned by: james
owned by: myers
mutable description: This is the coding sequence of ''crtY'' from ''Pantoea ananatis'' (formerly ''Erwinia uredovora'') (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118008
component: BBa_K118012
sequence annotation: BBa_K118012
sequence annotation: BBa_K118008
sequence: https://synbiohub.org/public/igem/BBa_K118013_sequence/1

BBa_K118008
type: Component
persistent identity: component1972438
display id: component1972438
version: 1
top level: BBa_K118013
definition: BBa_K118008
access: public

BBa_K118012
type: Component
persistent identity: component1972437
display id: component1972437
version: 1
top level: BBa_K118013
definition: BBa_K118012
access: public

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation1972437
display id: annotation1972437
version: 1
top level: BBa_K118013
location: https://synbiohub.org/public/igem/BBa_K118013/annotation1972437/range1972437/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K118013/annotation1972437/range1972437/1
type: Range
persistent identity: range1972437
display id: range1972437
version: 1
top level: BBa_K118013
direction: direction/reverse
start: 1
end: 4
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation1972438
display id: annotation1972438
version: 1
top level: BBa_K118013
location: https://synbiohub.org/public/igem/BBa_K118013/annotation1972438/range1972438/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K118013/annotation1972438/range1972438/1
type: Range
persistent identity: range1972438
display id: range1972438
version: 1
top level: BBa_K118013
direction: direction/reverse
start: 11
end: 1162
orientation: inline

BBa_K118014
type: ComponentDefinition
persistent identity: BBa K118014
display id: BBa_K118014
version: 1
was generated by: iGEM to SBOL conversion
description: rbs+crtE (geranylgeranyl pyrophosphate synthase)
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:37Z
mutable provenance: The synthetic ribosome binding site was added by fusion PCR to the ''crtE'' gene, which was amplified from ''Pantoea ananatis'' DSMZ 30080 (ATCC 19321) genomic DNA.
owned by: james
owned by: myers
mutable description: This is the coding sequence of ''crtE'' from ''Pantoea ananatis'' (formerly ''Erwinia uredovora'') (Accession number D90087). It encodes geranylgeranyl pyrophosphate synthase, part of the carotenoid biosynthesis pathway, which converts farnesyl diphosphate to geranylgeranyl diphosphate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The ''crtE'' BioBrick (BBa_I742151) had to be transferred from the non-compliant BABEL vector to a compliant pSB1A2 vector.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_I742151
component: BBa_K118012
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence: https://synbiohub.org/public/igem/BBa_K118014_sequence/1

BBa_I742151
type: Component
persistent identity: component1972440
display id: component1972440
version: 1
top level: BBa_K118014
definition: BBa_I742151
access: public

BBa_K118012
type: Component
persistent identity: component1972439
display id: component1972439
version: 1
top level: BBa_K118014
definition: BBa_K118012
access: public

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation1972439
display id: annotation1972439
version: 1
top level: BBa_K118014
location: https://synbiohub.org/public/igem/BBa_K118014/annotation1972439/range1972439/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K118014/annotation1972439/range1972439/1
type: Range
persistent identity: range1972439
display id: range1972439
version: 1
top level: BBa_K118014
direction: direction/reverse
start: 1
end: 4
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation1972440
display id: annotation1972440
version: 1
top level: BBa_K118014
location: https://synbiohub.org/public/igem/BBa_K118014/annotation1972440/range1972440/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K118014/annotation1972440/range1972440/1
type: Range
persistent identity: range1972440
display id: range1972440
version: 1
top level: BBa_K118014
direction: direction/reverse
start: 11
end: 922
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_I742151
type: ComponentDefinition
persistent identity: BBa I742151
display id: BBa_I742151
version: 1
was generated by: iGEM to SBOL conversion
description: crtE (geranylgeranyl pyrophosphate synthase) coding sequence.
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis parthway.
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No important considerations.
star: false
creator: Chris French
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I742151_sequence/1

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: iGEM to SBOL conversion
description: crtB coding sequence encoding phytoene synthase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
top level: BBa_K118002
direction: direction/forward
start: 1
end: 930
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: iGEM to SBOL conversion
description: crtI coding sequence encoding phytoene dehydrogenase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtI coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
top level: BBa_K118003
direction: direction/forward
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
top level: BBa_K118003
direction: direction/forward
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
top level: BBa_K118003
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_K118008
type: ComponentDefinition
persistent identity: BBa K118008
display id: BBa_K118008
version: 1
was generated by: iGEM to SBOL conversion
description: crtY coding sequence encoding lycopene B-cyclase
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118008_sequence/1

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995716
display id: annotation1995716
version: 1
top level: BBa_K118008
location: https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
type: Range
persistent identity: range1995716
display id: range1995716
version: 1
top level: BBa_K118008
direction: direction/forward
start: 1
end: 1149
orientation: inline

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ribosome binding site added by Son of Babel procedure
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
star: false
creator: Andrew Hall
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

https://synbiohub.org/public/igem/BBa_K2151201_sequence/1
type: Sequence
persistent identity: BBa K2151201 sequence
display id: BBa_K2151201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagctactagaggaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2151200_sequence/1
type: Sequence
persistent identity: BBa K2151200 sequence
display id: BBa_K2151200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118009_sequence/1
type: Sequence
persistent identity: BBa K118009 sequence
display id: BBa_K118009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118013_sequence/1
type: Sequence
persistent identity: BBa K118013 sequence
display id: BBa_K118013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118014_sequence/1
type: Sequence
persistent identity: BBa K118014 sequence
display id: BBa_K118014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742151_sequence/1
type: Sequence
persistent identity: BBa I742151 sequence
display id: BBa_I742151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118002_sequence/1
type: Sequence
persistent identity: BBa K118002 sequence
display id: BBa_K118002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118003_sequence/1
type: Sequence
persistent identity: BBa K118003 sequence
display id: BBa_K118003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118008_sequence/1
type: Sequence
persistent identity: BBa K118008 sequence
display id: BBa_K118008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118012_sequence/1
type: Sequence
persistent identity: BBa K118012 sequence
display id: BBa_K118012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z