BBa_K2152002
1
BBa_K2152002
CsgA-EGF
2016-10-12T11:00:00Z
2016-10-13T10:01:58Z
For CsgA, it is from a gift from Allen Y. Chen, Biophysics Program, Harvard University.
For EGF, it is synthesized by GenScript.
Through molecular programming of the bacterial extracellular matrix material by genetically appending EGF peptide to the amyloid protein CsgA, the dominant proteinaceous component in E. coli biofilms. These engineered CsgA fusion proteins are successfully secreted and extracellularly self-assemble into amyloid nanofibrer networks that retain the functions of the displayed peptide domains. It may be able to use the function of EGF to treat IBD.
false
false
_2622_
32588
32588
9
false
We use overlap PCR to connect CsgA and EGF with a linker. And to make a standard part, we performed site-directed mutagenesis to eliminate the restriction site of PstⅠ.
false
Shenjian Ai
annotation2497373
1
linker
range2497373
1
442
480
annotation2497375
1
EGF
range2497375
1
481
639
annotation2497437
1
his tag
range2497437
1
640
657
annotation2497372
1
CsgA
range2497372
1
1
441
BBa_K2152002_sequence
1
atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgccgttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagggtggcggtggctctggtggcggtggctctaattccgacagcgagtgtccgttgagtcacgacggctactgtctgcacgatggcgtttgcatgtatattgaagctctggacaaatacgcttgtaactgtgttgttggctacatcggtgaacgctgccagtacagagatctgaaatggtgggaactgcgtcaccaccaccaccaccac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z