LOCUS       BBa_K215210              702 bp    DNA     linear       22-Dec-2024
DEFINITION  IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin
ACCESSION   BBa_K215210
VERSION     BBa_K215210.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..168
                     /label=BBa_J36835 - Lpp Signaling Peptide
     misc_feature    175..237
                     /label=BBa_J36836 - 1tmr OmpA
     misc_feature    238..270
                     /label=GS Linker - GGGSGGGSGGG
     misc_feature    271..291
                     /label=TEV Site - ENLYFQG
     misc_feature    298..702
                     /label=BBa_J36841 - WT Streptavidin w/o start codon
     CDS             82..702
                     /label=BBa_K215260
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaagct actaaactgg tactgggcgc ggtaatcctg
      121 ggttctactc tgctggcagg ttgctccagc aacgctaaaa tcgatcagac tagaaacccg
      181 tatgttggct ttgaaatggg ttacgactgg ttaggtcgta tgccgtacaa aggcagcggt
      241 ggtggttctg gtggtggttc tggtggtggt gaaaacctgt attttcaggg tgctagagct
      301 gaagctggta tcaccggcac ctggtacaac cagctgggat ccaccttcat cgttaccgct
      361 ggtgctgacg gtgctctgac cggtacctac gaatccgctg ttggtaacgc tgaaagccgc
      421 tacgttctga ccggtcgtta cgactccgct ccggctaccg acggttccgg aaccgctctg
      481 ggttggaccg ttgcttggaa aaacaactac cgtaacgctc actccgctac cacctggtct
      541 ggccagtacg ttggtggtgc tgaagctcgt atcaacaccc agtggttgtt gacctccggc
      601 accaccgaag ccaacgcgtg gaaatccacc ctggttggtc acgacacctt caccaaagtt
      661 aaaccgtccg ctgcttccca tcaccatcac caccattaat aa
//