##gff-version 3 ##sequence-region BBa_K215210 1 702 BBa_K215210 . promoter 1 54 . + 0 ID=BBa_R0011;Name=BBa_R0011 BBa_K215210 . promoter 43 48 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_K215210 . engineered_region 1 54 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_K215210 . operator 3 19 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_K215210 . promoter 20 25 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_K215210 . operator 26 42 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_K215210 . ribosome_entry_site 64 75 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K215210 . conserved_region 68 71 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K215210 . CDS 82 702 . + 0 ID=BBa_K215260;Name=BBa_K215260 BBa_K215210 . sequence_feature 238 270 . + 0 ID=annotation2027685;Name=GS Linker - GGGSGGGSGGG;Parent=BBa_K215260 BBa_K215210 . engineered_region 298 702 . + 0 ID=annotation2027687;Name=BBa_J36841 - WT Streptavidin w/o start codon;Parent=BBa_K215260 BBa_K215210 . engineered_region 175 237 . + 0 ID=annotation2027684;Name=BBa_J36836 - 1tmr OmpA;Parent=BBa_K215260 BBa_K215210 . sequence_feature 271 291 . + 0 ID=annotation2027686;Name=TEV Site - ENLYFQG;Parent=BBa_K215260 BBa_K215210 . engineered_region 82 168 . + 0 ID=annotation2027683;Name=BBa_J36835 - Lpp Signaling Peptide;Parent=BBa_K215260 >BBa_K215210 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact agatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaa aatcgatcagactagaaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagc ggtggtggttctggtggtggttctggtggtggtgaaaacctgtattttcagggtgctagagctgaagctggtatcaccg gcacctggtacaaccagctgggatccaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatc cgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaacc gctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttg gtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccct ggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa