LOCUS       BBa_K215211              981 bp    DNA     linear       25-Nov-2024
DEFINITION  IPTG Inducible 5tmr OmpA Surface Display of WT Streptavidin
ACCESSION   BBa_K215211
VERSION     BBa_K215211.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..168
                     /label=BBa_J36835 - Lpp Signaling Peptide
     misc_feature    175..516
                     /label=BBa_J36837 - 5tmr OmpA
     misc_feature    517..549
                     /label=GS Linker - GGGSGGGSGGG
     misc_feature    550..570
                     /label=TEV Site - ENLYFQG
     misc_feature    577..981
                     /label=BBa_J36841 - WT Streptavidin w/o start codon
     CDS             82..981
                     /label=BBa_K215261
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaagct actaaactgg tactgggcgc ggtaatcctg
      121 ggttctactc tgctggcagg ttgctccagc aacgctaaaa tcgatcagac tagaaacccg
      181 tatgttggct ttgaaatggg ttacgactgg ttaggtcgta tgccgtacaa aggcagcgtt
      241 gaaaacggtg catacaaagc tcagggcgtt caactgaccg ctaaactggg ttacccaatc
      301 actgacgacc tggacatcta cactcgtctg ggtggcatgg tatggcgtgc agacactaaa
      361 tccaacgttt atggtaaaaa ccacgacacc ggcgtttctc cggtcttcgc tggcggtgtt
      421 gagtacgcga tcactcctga aatcgctacc cgtctggaat accagtggac caacaacatc
      481 ggtgacgcac acaccatcgg cactcgtccg gacaacggtg gtggttctgg tggtggttct
      541 ggtggtggtg aaaacctgta ttttcagggt gctagagctg aagctggtat caccggcacc
      601 tggtacaacc agctgggatc caccttcatc gttaccgctg gtgctgacgg tgctctgacc
      661 ggtacctacg aatccgctgt tggtaacgct gaaagccgct acgttctgac cggtcgttac
      721 gactccgctc cggctaccga cggttccgga accgctctgg gttggaccgt tgcttggaaa
      781 aacaactacc gtaacgctca ctccgctacc acctggtctg gccagtacgt tggtggtgct
      841 gaagctcgta tcaacaccca gtggttgttg acctccggca ccaccgaagc caacgcgtgg
      901 aaatccaccc tggttggtca cgacaccttc accaaagtta aaccgtccgc tgcttcccat
      961 caccatcacc accattaata a
//