##gff-version 3
##sequence-region BBa_K215211 1 981
BBa_K215211	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K215211	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K215211	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K215211	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K215211	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K215211	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K215211	.	CDS	82	981	.	+	0	ID=BBa_K215261;Name=BBa_K215261
BBa_K215211	.	engineered_region	82	168	.	+	0	ID=annotation2027701;Name=BBa_J36835 - Lpp Signaling Peptide;Parent=BBa_K215261
BBa_K215211	.	sequence_feature	517	549	.	+	0	ID=annotation2027703;Name=GS Linker - GGGSGGGSGGG;Parent=BBa_K215261
BBa_K215211	.	sequence_feature	577	981	.	+	0	ID=annotation2027705;Name=BBa_J36841 - WT Streptavidin w/o start codon;Parent=BBa_K215261
BBa_K215211	.	engineered_region	175	516	.	+	0	ID=annotation2027702;Name=BBa_J36837 - 5tmr OmpA;Parent=BBa_K215261
BBa_K215211	.	sequence_feature	550	570	.	+	0	ID=annotation2027704;Name=TEV Site - ENLYFQG;Parent=BBa_K215261
BBa_K215211	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K215211	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K215211
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaa
aatcgatcagactagaaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagc
gttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggaca
tctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgt
ttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaac
aacatcggtgacgcacacaccatcggcactcgtccggacaacggtggtggttctggtggtggttctggtggtggtgaaa
acctgtattttcagggtgctagagctgaagctggtatcaccggcacctggtacaaccagctgggatccaccttcatcgt
taccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgacc
ggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactacc
gtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgtt
gacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtcc
gctgcttcccatcaccatcaccaccattaataa