BBa_K2155001 1 BBa_K2155001 BFD-Benzoylformate Decarboxylase 2016-10-08T11:00:00Z 2016-10-18T02:45:21Z Benzoylformate decarboxylase(BFD) comes from Pseudomonas putida, which could cleave carbon-carbon bonds. In enzymology, a benzoylformate decarboxylase (EC 4.1.1.7) is an enzyme that catalyzes the following chemical reaction: benzoylformate + H+ ⇌ benzaldehyde + CO2 However, in our project we get an engineered benzoylformate decarboxylase, which could catalyze formaldehyde into Glycolaldehyde and Dihydroxyacetone, from CAS. If you use it to catalyze reaction in vivo, It needs one cofactor, thiamin diphosphate. false false _2625_ 31739 31739 9 false Benzoylformate decarboxylase is flanked by prefix and suffix provided by iGEM. false Bingzhao Zhuo, Yue Yang BBa_K2155001_sequence 1 atggcttctgttcacggtaccacctacgaactgctgcgtcgtcagggtatcgacaccgttttcggtaacccgggttctaacgaactgccgttcctgaaagacttcccggaagacttccgttacatcctggctcttcaggaagcttgcgttgttggtatcgctgacggttacgctcaggcttctcgtaaaccggctttcatcaacctgcactctgctgctggtaccggtaacgctatgggtgctctgtctaacgcttggaactctcactctccgctgatcgttaccgctggtcagcagacccgtgctatgatcggtgttgaagctctgctgaccaacgttgacgctgctaacctgccgcgtccgctggttaaatggtcttacgaaccggcttctgctgctgaagttccgcacgctatgtctcgtgctatccacatggcttctatggctccgcagggtccggtttacctgtctgttccgtacgacgactgggacaaagacgctgacccgcagtctcaccacctgttcgaccgtcacgtttcttcttctgttcgtctgaacgaccaggacctggacatcctggttaaagctctgaactctgcttctaacccggctatcgttctgggtccggacgttgacgctgctaacgctaacgctgactgcgttatgctggctgaacgtctgaaagctccggtttgggttgctccgtctgctccgcgttgcccgttcccgacccgtcacccgtgcttccgtggtctgatgccggctggtatcgctgctatctctcagctgctggaaggtcacgacgttgttctggttatcggtgctccggttttccgttaccaccagtacgacccgggtcagtacctgaaaccgggtacccgtctgatctctgttacctgcgacccgctggaagctgctcgtgctccgatgggtgacgctatcgttgctgacatcggtgctatggcttctgctctggctaacctggttgaagaatcttctcgtcagctgccgaccgctgctccggaaccggctaaagttgaccaggacgctggtcgtctgcacccggaaaccgttttcgacaccctgaacgacatggctccggaaaacgctatctacctgaacgaatctacctctaccaccgctcagatgtggcagcgtctgaacatgcgtaacccgggttcttactacttctgcgctgctggtggtctgggtttcgctctgccggctgctatcggtgttcagctggctgaaccggaacgtcaggttatcgctgttatcggtgacggttctgctaactactctatctctgctctgtggaccgctgctcagtacaacatcccgaccatcttcgttatcatgaacaacggtacctacggtgctctgcgttggttcgctggtgttctggaagctgaaaacgttccgggtctggacgttccgggtatcgacttccgtgctctggctaaaggttacggtgttcaggctctgaaagctgacaacctggaacagctgaaaggttctcttcaggaagctctgtctgctaaaggtccggttctgatcgaagtttctaccgtttctccggttaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z