BBa_K2155002 1 BBa_K2155002 CtDock 2016-10-08T11:00:00Z 2016-10-18T02:54:58Z C.thermocellum It is from C.thermocellum CelS (673−741 amino acids, GenBank Accession number: L06942), can automatically assemble with a cohesin module which is from CipA false false _2625_ 31739 31739 9 false Scaffold false Xin Yin， Bingzhao Zhuo BBa_K2155001 1 BBa_K2155001 BFD-Benzoylformate Decarboxylase 2016-10-08T11:00:00Z 2016-10-18T02:45:21Z Benzoylformate decarboxylase(BFD) comes from Pseudomonas putida, which could cleave carbon-carbon bonds. In enzymology, a benzoylformate decarboxylase (EC 4.1.1.7) is an enzyme that catalyzes the following chemical reaction: benzoylformate + H+ ⇌ benzaldehyde + CO2 However, in our project we get an engineered benzoylformate decarboxylase, which could catalyze formaldehyde into Glycolaldehyde and Dihydroxyacetone, from CAS. If you use it to catalyze reaction in vivo, It needs one cofactor, thiamin diphosphate. false false _2625_ 31739 31739 9 false Benzoylformate decarboxylase is flanked by prefix and suffix provided by iGEM. false Bingzhao Zhuo, Yue Yang BBa_K2155010 1 BBa_K2155010 BFD-CtDock 2016-10-09T11:00:00Z 2016-10-19T07:25:26Z BFD is from Pseudomonas putida. CtDocS is from C.thermocellum ATCC 27405 This complex is constituted of BFD and CtDocS. CtDocS is a dockers ,which could bind to scaffold protein we used in this project. false false _2625_ 31739 31739 9 false Benzoylformate decarboxylase is flanked by prefix and suffix provided by iGEM. false BingZhao Zhuo, Yue Yang, Zexun Wu component2491646 1 BBa_K2155002 component2491645 1 BBa_K2155001 annotation2491646 1 BBa_K2155002 range2491646 1 1593 1880 annotation2491645 1 BBa_K2155001 range2491645 1 1 1584 BBa_K2155010_sequence 1 atggcttctgttcacggtaccacctacgaactgctgcgtcgtcagggtatcgacaccgttttcggtaacccgggttctaacgaactgccgttcctgaaagacttcccggaagacttccgttacatcctggctcttcaggaagcttgcgttgttggtatcgctgacggttacgctcaggcttctcgtaaaccggctttcatcaacctgcactctgctgctggtaccggtaacgctatgggtgctctgtctaacgcttggaactctcactctccgctgatcgttaccgctggtcagcagacccgtgctatgatcggtgttgaagctctgctgaccaacgttgacgctgctaacctgccgcgtccgctggttaaatggtcttacgaaccggcttctgctgctgaagttccgcacgctatgtctcgtgctatccacatggcttctatggctccgcagggtccggtttacctgtctgttccgtacgacgactgggacaaagacgctgacccgcagtctcaccacctgttcgaccgtcacgtttcttcttctgttcgtctgaacgaccaggacctggacatcctggttaaagctctgaactctgcttctaacccggctatcgttctgggtccggacgttgacgctgctaacgctaacgctgactgcgttatgctggctgaacgtctgaaagctccggtttgggttgctccgtctgctccgcgttgcccgttcccgacccgtcacccgtgcttccgtggtctgatgccggctggtatcgctgctatctctcagctgctggaaggtcacgacgttgttctggttatcggtgctccggttttccgttaccaccagtacgacccgggtcagtacctgaaaccgggtacccgtctgatctctgttacctgcgacccgctggaagctgctcgtgctccgatgggtgacgctatcgttgctgacatcggtgctatggcttctgctctggctaacctggttgaagaatcttctcgtcagctgccgaccgctgctccggaaccggctaaagttgaccaggacgctggtcgtctgcacccggaaaccgttttcgacaccctgaacgacatggctccggaaaacgctatctacctgaacgaatctacctctaccaccgctcagatgtggcagcgtctgaacatgcgtaacccgggttcttactacttctgcgctgctggtggtctgggtttcgctctgccggctgctatcggtgttcagctggctgaaccggaacgtcaggttatcgctgttatcggtgacggttctgctaactactctatctctgctctgtggaccgctgctcagtacaacatcccgaccatcttcgttatcatgaacaacggtacctacggtgctctgcgttggttcgctggtgttctggaagctgaaaacgttccgggtctggacgttccgggtatcgacttccgtgctctggctaaaggttacggtgttcaggctctgaaagctgacaacctggaacagctgaaaggttctcttcaggaagctctgtctgctaaaggtccggttctgatcgaagtttctaccgtttctccggttaaatactagaggttgattggaacaaggcaactgcttatataagtggaaaacttaattggggtatcgaaccaggatctggaggaggtgggtcctctactaaattatacggcgacgtcaatgatgacggaaaagttaactcaactgacgctgtagcattgaagagatatgttttgagatcaggtataagcatcaacactgacaatgccgatttgaatgaagacggcagagttaattcaactgacttaggaattttgaagagatatattctcaaagaaatagatacattgccgtacaagaac BBa_K2155001_sequence 1 atggcttctgttcacggtaccacctacgaactgctgcgtcgtcagggtatcgacaccgttttcggtaacccgggttctaacgaactgccgttcctgaaagacttcccggaagacttccgttacatcctggctcttcaggaagcttgcgttgttggtatcgctgacggttacgctcaggcttctcgtaaaccggctttcatcaacctgcactctgctgctggtaccggtaacgctatgggtgctctgtctaacgcttggaactctcactctccgctgatcgttaccgctggtcagcagacccgtgctatgatcggtgttgaagctctgctgaccaacgttgacgctgctaacctgccgcgtccgctggttaaatggtcttacgaaccggcttctgctgctgaagttccgcacgctatgtctcgtgctatccacatggcttctatggctccgcagggtccggtttacctgtctgttccgtacgacgactgggacaaagacgctgacccgcagtctcaccacctgttcgaccgtcacgtttcttcttctgttcgtctgaacgaccaggacctggacatcctggttaaagctctgaactctgcttctaacccggctatcgttctgggtccggacgttgacgctgctaacgctaacgctgactgcgttatgctggctgaacgtctgaaagctccggtttgggttgctccgtctgctccgcgttgcccgttcccgacccgtcacccgtgcttccgtggtctgatgccggctggtatcgctgctatctctcagctgctggaaggtcacgacgttgttctggttatcggtgctccggttttccgttaccaccagtacgacccgggtcagtacctgaaaccgggtacccgtctgatctctgttacctgcgacccgctggaagctgctcgtgctccgatgggtgacgctatcgttgctgacatcggtgctatggcttctgctctggctaacctggttgaagaatcttctcgtcagctgccgaccgctgctccggaaccggctaaagttgaccaggacgctggtcgtctgcacccggaaaccgttttcgacaccctgaacgacatggctccggaaaacgctatctacctgaacgaatctacctctaccaccgctcagatgtggcagcgtctgaacatgcgtaacccgggttcttactacttctgcgctgctggtggtctgggtttcgctctgccggctgctatcggtgttcagctggctgaaccggaacgtcaggttatcgctgttatcggtgacggttctgctaactactctatctctgctctgtggaccgctgctcagtacaacatcccgaccatcttcgttatcatgaacaacggtacctacggtgctctgcgttggttcgctggtgttctggaagctgaaaacgttccgggtctggacgttccgggtatcgacttccgtgctctggctaaaggttacggtgttcaggctctgaaagctgacaacctggaacagctgaaaggttctcttcaggaagctctgtctgctaaaggtccggttctgatcgaagtttctaccgtttctccggttaaa BBa_K2155002_sequence 1 gttgattggaacaaggcaactgcttatataagtggaaaacttaattggggtatcgaaccaggatctggaggaggtgggtcctctactaaattatacggcgacgtcaatgatgacggaaaagttaactcaactgacgctgtagcattgaagagatatgttttgagatcaggtataagcatcaacactgacaatgccgatttgaatgaagacggcagagttaattcaactgacttaggaattttgaagagatatattctcaaagaaatagatacattgccgtacaagaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z