BBa_K823005 1 BBa_K823005 Anderson promoter J23101 2012-09-07T11:00:00Z 2015-07-07T11:44:25Z Partsregistry Released HQ 2013 Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon. false false _1081_ 4206 12081 9 In stock false no considerations false Korinna Kraft annotation2182510 1 -35 box range2182510 1 1 6 annotation2182509 1 J23101 range2182509 1 1 35 annotation2182511 1 -10 box range2182511 1 24 29 BBa_K2156831 1 LuxB LuxB 2016-08-24T11:00:00Z 2016-08-25T03:26:59Z AF403784.1 (GeneBank) LuxB part, ordered from IDT (Integrated DNA Technologies) false false _2626_ 31855 31855 9 false Requires the addition of prefixes and suffixes false Carolina Sofia Gonzalez and Mikhail Lujan BBa_K2156666 1 LuxA LuxA 2016-08-24T11:00:00Z 2016-08-25T03:53:32Z AF403784.1 (Genbank) LuxA part, ordered from IDT (Integrated DNA Technologies) false false _2626_ 31855 31855 9 false Used prefixes and suffixes false Carolina Sofia Gonzalez and Mikhail Lujan BBa_K1725301 1 BBa_K1725301 Member of the RBS library - derived from BBa_B0032 2015-09-10T11:00:00Z 2015-09-18T02:50:20Z - Parts K1725225-K1725256 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR. false false _2146_ 27362 27362 9 false - false Vilija Lomeikaite annotation2476133 1 RBS range2476133 1 1 13 BBa_K2156017 1 BBa_K2156017 Lux ABG with promoter and RBS 2016-09-21T11:00:00Z 2016-09-22T02:40:32Z iGEM Glasgow, GenBank (see in each part) LuxABG is a combination of the separate LuxA, LuxB and LuxG parts, including the relevant Ribosome Binding Site (BBa_K1725301) and an Anderson Promoter (BBa_K823005). LuxAB is the required pair for an operational lux, with LuxG decreasing the energy required for bioluminescence. LuxC and Lux D are needed to synthesize and replenish the aldehyde substrate needed for bioluminescence. This was synthesized by IDT (Integrated DNA Technologies). false false _2626_ 31855 31855 9 false Requires LuxCD to be fully functional false Carolina Sofia Gonzalez and Mikhail Lujan component2484514 1 BBa_K823005 component2484525 1 BBa_K2156104 component2484517 1 BBa_K2156666 component2484516 1 BBa_K1725301 annotation2484517 1 BBa_K2156666 range2484517 1 63 1142 annotation2484525 1 BBa_K2156104 range2484525 1 1151 2882 annotation2484514 1 BBa_K823005 range2484514 1 1 35 annotation2484516 1 BBa_K1725301 range2484516 1 44 56 BBa_K1725205 1 luxG LuxG 2015-08-03T11:00:00Z 2015-09-18T11:35:46Z Test Test false false _2146_ 20827 27303 9 false Test false Charlotte Flynn annotation2434651 1 LuxG range2434651 1 1 711 BBa_K2156104 1 LuxBG LuxBG with Ribosome Binding Site 2016-08-24T11:00:00Z 2016-08-25T03:32:51Z AF403784.1 (GenBank) BBa_K1725205 (iGem Registry) LuxBG is a combination of the separate LuxB and LuxG parts, including the relevant Ribosome Binding Site (BBa_K1725301) and the official iGem prefix and suffix. This part is listed as an intermediate because it requires LuxA for functional bioluminescence. LuxAB is the required pair for an operational lux, with LuxG decreasing the energy required for bioluminescence. This was synthesized by IDT (Integrated DNA Technologies). false false _2626_ 31855 31855 9 false The addition of a Ribosome Binding Site, prefixes, and suffixes. false Carolina Sofia Gonzalez and Mikhail Lujan component2481611 1 BBa_K1725205 component2481609 1 BBa_K1725301 component2481607 1 BBa_K2156831 component2481606 1 BBa_K1725301 annotation2481611 1 BBa_K1725205 range2481611 1 1022 1732 annotation2481609 1 BBa_K1725301 range2481609 1 1003 1015 annotation2481606 1 BBa_K1725301 range2481606 1 1 13 annotation2481607 1 BBa_K2156831 range2481607 1 20 994 BBa_K1725205_sequence 1 atgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa BBa_K2156104_sequence 1 tcacacaggagggtactagatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttggtgtatgaaaatcatttttcaggtaatggtgttgtcggtgctcctctgactgtttctggttttttgctcggtttaacagaaaaaattaaaattggctcattgaatcacatcattacaactcatcatcctgtccgaatagcggaggaggcttgcttactggatcaattaagcgaagggagatttattttagggtttagtgattgtgaaaaaaaagatgaaatgcgtctttttaatcgccctgttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatcccgataatgatttttatagtttccctaaaatatcggtaaacccccacgcttatacccaaggcgggcctcggagatatattacagcaaccagtcatcatattgttgaatgggcggctaaaaaaggcattcctctcatctttaagtgggatgactccaatgatgttagatatgaatatgctgaaaggtataaagccgttgctgataaatatggcattgacttatcagcgatagatcatcagttaatggtattggttaactataacgaagatagtcacaaagctaaacaagagacgcgtgcatttatccgtgattatgttcttgaaatgtatcctaatgaaaatctcgaaaataaacttgaagagataatcacagaaaacgctgtcggagattatacggaatgtatagctgcggctaagctggcaattgaaaagtgcggtgcaaaaagggtattattatcctttgaaccaatgaatgacttgatgcaccaaaaaaatgtaatcaatattgttgatgataatattaaaaagtaccacatgtagtactagagtcacacaggagggtactagatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa BBa_K823005_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K2156831_sequence 1 atgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttggtgtatgaaaatcatttttcaggtaatggtgttgtcggtgctcctctgactgtttctggttttttgctcggtttaacagaaaaaattaaaattggctcattgaatcacatcattacaactcatcatcctgtccgaatagcggaggaggcttgcttactggatcaattaagcgaagggagatttattttagggtttagtgattgtgaaaaaaaagatgaaatgcgtctttttaatcgccctgttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatcccgataatgatttttatagtttccctaaaatatcggtaaacccccacgcttatacccaaggcgggcctcggagatatattacagcaaccagtcatcatattgttgaatgggcggctaaaaaaggcattcctctcatctttaagtgggatgactccaatgatgttagatatgaatatgctgaaaggtataaagccgttgctgataaatatggcattgacttatcagcgatagatcatcagttaatggtattggttaactataacgaagatagtcacaaagctaaacaagagacgcgtgcatttatccgtgattatgttcttgaaatgtatcctaatgaaaatctcgaaaataaacttgaagagataatcacagaaaacgctgtcggagattatacggaatgtatagctgcggctaagctggcaattgaaaagtgcggtgcaaaaagggtattattatcctttgaaccaatgaatgacttgatgcaccaaaaaaatgtaatcaatattgttgatgataatattaaaaagtaccacatgtag BBa_K2156017_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggagggtactagatgaaatttggaaactttttgcttacataccaacccccccaattttctcaaacagaagtaatgaaacgtttggttaaattaggtcgtatttctgaggagtgtggttttgatactgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggtgcaaccaaaaaattgaatgtagggactgcggctattgttcttcccaccgctcatccagtgcgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgtcgggggctttacaataaagactttcgcgtatttggcacggatatgaataacagtcgcgctttaacggagtgctggtacgggttgataaaaaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaacccgacagcatatagtaaaggtggagcccctgtttatgtggttgctgaatcagcctcgacaactgaatgggccgctcaatttggtttaccgatgatattaagttggattataaatactaacgaaaagaaagcacagcttgagctttataacgaggtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtaaattatgactcaaataaagcgaaagagatttgtcggaaatttctagggcattggtatgattcttatgtgaatgccacgaccatttttgatgattcagacaaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaagggacatagagatactaatcgccgcattgattacagttacgaaatcaatcccgtgggaaccccgcaggaatgcattgacataattcaaaaagacattgatgccacgggaatatcaaatatctgttgtgggtttgaagcgaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccgtttcttaaagaaaaacaacgttcgctattatagtactagagtcacacaggagggtactagatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttggtgtatgaaaatcatttttcaggtaatggtgttgtcggtgctcctctgactgtttctggttttttgctcggtttaacagaaaaaattaaaattggctcattgaatcacatcattacaactcatcatcctgtccgaatagcggaggaggcttgcttactggatcaattaagcgaagggagatttattttagggtttagtgattgtgaaaaaaaagatgaaatgcgtctttttaatcgccctgttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatcccgataatgatttttatagtttccctaaaatatcggtaaacccccacgcttatacccaaggcgggcctcggagatatattacagcaaccagtcatcatattgttgaatgggcggctaaaaaaggcattcctctcatctttaagtgggatgactccaatgatgttagatatgaatatgctgaaaggtataaagccgttgctgataaatatggcattgacttatcagcgatagatcatcagttaatggtattggttaactataacgaagatagtcacaaagctaaacaagagacgcgtgcatttatccgtgattatgttcttgaaatgtatcctaatgaaaatctcgaaaataaacttgaagagataatcacagaaaacgctgtcggagattatacggaatgtatagctgcggctaagctggcaattgaaaagtgcggtgcaaaaagggtattattatcctttgaaccaatgaatgacttgatgcaccaaaaaaatgtaatcaatattgttgatgataatattaaaaagtaccacatgtagtactagagtcacacaggagggtactagatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa BBa_K2156666_sequence 1 atgaaatttggaaactttttgcttacataccaacccccccaattttctcaaacagaagtaatgaaacgtttggttaaattaggtcgtatttctgaggagtgtggttttgatactgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggtgcaaccaaaaaattgaatgtagggactgcggctattgttcttcccaccgctcatccagtgcgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgtcgggggctttacaataaagactttcgcgtatttggcacggatatgaataacagtcgcgctttaacggagtgctggtacgggttgataaaaaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaacccgacagcatatagtaaaggtggagcccctgtttatgtggttgctgaatcagcctcgacaactgaatgggccgctcaatttggtttaccgatgatattaagttggattataaatactaacgaaaagaaagcacagcttgagctttataacgaggtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtaaattatgactcaaataaagcgaaagagatttgtcggaaatttctagggcattggtatgattcttatgtgaatgccacgaccatttttgatgattcagacaaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaagggacatagagatactaatcgccgcattgattacagttacgaaatcaatcccgtgggaaccccgcaggaatgcattgacataattcaaaaagacattgatgccacgggaatatcaaatatctgttgtgggtttgaagcgaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccgtttcttaaagaaaaacaacgttcgctattatag BBa_K1725301_sequence 1 tcacacaggaggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z