The part is the complete combination of the Lux's A, B, G, C, D, E. This construct is meant to produce Bioluminescence, with the LuxAB beginning the structure, LuxG removing energy pathways to make the reaction more efficient, and LuxCDE synthesizing necessary substrates for the reaction.
The addition of RBS and promoter to the construct.
Mikhail Lujan and Carolina Sofia Gonzalez
Member of the RBS library - derived from BBa_B0032
Parts K1725225-K1725256 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR.
LuxC part, ordered from IDT (Integrated DNA Technologies)
Carolina Sofia Gonzalez and Mikhail Lujan
LuxD part, ordered from IDT (Integrated DNA Technologies)
LuxE part, ordered from IDT (Integrated DNA Technologies)
iGEM Glasgow, GenBank (see in each part)
LuxABG is a combination of the separate LuxA, LuxB and LuxG parts, including the relevant Ribosome Binding Site (BBa_K1725301) and an Anderson Promoter (BBa_K823005). LuxAB is the required pair for an operational lux, with LuxG decreasing the energy required for bioluminescence. LuxC and Lux D are needed to synthesize and replenish the aldehyde substrate needed for bioluminescence. This was synthesized by IDT (Integrated DNA Technologies).
AF403784.1 (GenBank) BBa_K1725205 (iGem Registry)
LuxBG is a combination of the separate LuxB and LuxG parts, including the relevant Ribosome Binding Site (BBa_K1725301) and the official iGem prefix and suffix. This part is listed as an intermediate because it requires LuxA for functional bioluminescence. LuxAB is the required pair for an operational lux, with LuxG decreasing the energy required for bioluminescence. This was synthesized by IDT (Integrated DNA Technologies).
The addition of a Ribosome Binding Site, prefixes, and suffixes.
LuxA part, ordered from IDT (Integrated DNA Technologies)
Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
LuxB part, ordered from IDT (Integrated DNA Technologies)
Requires the addition of prefixes and suffixes
Conversion of the iGEM parts registry to SBOL2.1