LOCUS       BBa_K2158004            1848 bp    DNA     linear       06-Nov-2024
DEFINITION  Uricase (from <i>B.subtilis</i>)
ACCESSION   BBa_K2158004
VERSION     BBa_K2158004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1711
                     /label=BBa_K2158000
     stem_loop       1731..1774
                     /label=stem_loop
     misc_feature    1720..1799
                     /label=BBa_B0010
     terminator      1720..1799
                     /label=BBa_B0010
     stem_loop       1815..1834
                     /label=T7 TE
     misc_feature    1841..1841
                     /label=stop
     polyA_site      1835..1848
                     /label=polya
     misc_feature    1808..1848
                     /label=BBa_B0012
     terminator      1808..1848
                     /label=BBa_B0012
     terminator      1720..1848
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt tcacaatgga
      241 tgacctgaac caaatggaca cacaaacact gacagacaca cttggctcta tttttgaaca
      301 ctcttcatgg attgcggaga gatccgcagc actacggccg ttttcgtccc tttctgatct
      361 tcaccgcaaa atgactggca ttgtaaaagc tgcggatcgc gagacacagc ttgatttaat
      421 caaaaagcat ccccggctcg gaacaaagaa aacaatgagc gatgactcgg tacgagagca
      481 gcagaacgca gggctcggca aattagaaca gcaggaatac gaggagtttc tcatgctgaa
      541 tgaacactat tatgatcgct tcggctttcc ttttatttta gcggtgaagg gaaagacgaa
      601 acaggacatt caccaagccc tgctggcaag gcttgagagc gaacgagaaa cggagttcca
      661 gcaggccctt atagaaattt accgaatcgc ccgctttcgt ctggctgaca tcatcactga
      721 aaaaggagag acgcaaatga aaagaaccat gtcttatggc aaaggaaatg tatttgcata
      781 ccgaacgtat ttaaaaccgc tcactggagt gaagcaaatc ccggaatcat cttttgccgg
      841 aagagataat accgttgtcg gcgttgatgt gacatgcgaa attggcggag aagccttcct
      901 gccatcattt acagacggag acaacactct cgtcgtggca acggattcaa tgaaaaactt
      961 tatccagcgc catctcgcat cctatgaagg aacgacaacc gaggggttcc tacactatgt
     1021 agctcaccga tttttagata cctattctca tatggacacg atcactctga ctggcgaaga
     1081 cattccgttt gaagcaatgc ctgcatacga ggagaaagag ctcagcacaa gccgcctcgt
     1141 cttcagaaga tcgcgtaatg aacgaagccg ctctgtgctg aaagcagaac gaagcgggaa
     1201 taccataacg attacagagc aatacagcga aatcatggat cttcagctcg tcaaggtgag
     1261 cggcaactcc ttcgtcggct tcatccggga cgaatatacg actctcccgg aagacggcaa
     1321 ccgcccgctg tttgtctatt taaacatcag ctggcaatat gaaaatacaa atgactcata
     1381 cgcttctgat ccagcacgct acgttgcggc tgaacaagtc cgcgacttag cgagcaccgt
     1441 ttttcacgag ctcgaaaccc cttcaatcca aaacctaatc tatcatatcg gctgtagaat
     1501 attagcgagg ttcccgcagc tcactgatgt cagcttccaa tctcaaaatc acacgtggga
     1561 tacggttgtc gaagaaatcc cgggctcaaa aggaaaagtc tacaccgaac cgcgcccgcc
     1621 atacggtttc caacatttta ccgtgacaag agaagacgcc gagaaagaaa aacagaaagc
     1681 cgctgaaaaa tgtcggagcc tgaaagcctg atactagagc caggcatcaa ataaaacgaa
     1741 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1801 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//