LOCUS       BBa_K2158005             357 bp    DNA     linear       16-Dec-2024
DEFINITION  Histidine tag + Uricase from <i>B.subtilis
ACCESSION   BBa_K2158005
VERSION     BBa_K2158005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     stem_loop       240..283
                     /label=stem_loop
     misc_feature    229..308
                     /label=BBa_B0010
     terminator      229..308
                     /label=BBa_B0010
     stem_loop       324..343
                     /label=T7 TE
     misc_feature    350..350
                     /label=stop
     polyA_site      344..357
                     /label=polya
     misc_feature    317..357
                     /label=BBa_B0012
     terminator      317..357
                     /label=BBa_B0012
     terminator      229..357
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagcc aggcatcaaa
      241 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      301 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//