BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K216000 1 BBa_K216000 luxB coding sequence from Photobacterium phosphoreum ATCC11060 2009-06-19T11:00:00Z 2015-05-08T01:11:30Z _Photobacterium phosphoreum_ ATCC 11060 GenBank accession DQ988873 Gene luxB encoding subunit B of the bacterial luciferase LuxAB of _Photobacterium phosphoreum_ ATCC 11060. This is a particularly bright organism and we hope that its luciferase will be much bright than the bacterial luciferase of _Photorhabdus luminescens_ which is already in the Registry. false false _317_ 0 837 163 Not in stock false No special considerations. Be aware that luminescence requires both LuxA and LuxB. If LuxC, LuxD and LuxE are also supplied, the organism can generate its own substrate, but otherwise, a long chain aldehyde such as n-decanal must be added. Addition of flavin reduction LuxG may or may not increase brightness. false Chris French BBa_K216001 1 BBa_K216001 Photobacterium phosphoreum luxB gene with RBS B0034 2009-06-19T11:00:00Z 2015-05-08T01:11:30Z Coding sequence from P. phosphoreum ATCC 11060 This is subunit B of luciferase from Photobacterium phosphoreum ATCC 11060, with the addition of ribosome binding site B0034 false false _317_ 0 837 163 Not in stock false No special considerations. false Chris French component2006141 1 BBa_K216000 component2006140 1 BBa_B0034 annotation2006141 1 BBa_K216000 range2006141 1 19 1008 annotation2006140 1 BBa_B0034 range2006140 1 1 12 BBa_K216001_sequence 1 aaagaggagaaatactagatgaattttggattattcttcctcaactttcagcctgaaaacacatcgtcagaaactgttttagataatatgatcaatatggtgtctctggttgataaagattataaaaactttacaactattttagtcaatgagcaccatttttctaaaaatggtattgtcggtgccccgatcactgctgcgagcttcctattagggttaactgaacgtttacacattggttctttaaatcaagttattacaactcatcacccggttcgtattgcagaagaagcaagtttacttgaccaaatgtcagatagtcgttttattctaggtttaagtgattgtattaatgattttgagatggatttctttaaacgtcagcgtgattcacagcagctacaatttgaagcttgttatgagatcattaatgaagcaatcacaaccaattattgccaagcgaataatgatttttataacttccctcgcatctcaattaatcctcattgcctgagtaaagagaatatgaagcaatatattttagcttctagtgtgagtgttgttgagtgggcggctaaaaaagcgctgccactgacatatcgttggagcgatagccttgaagataaagagattctttataagcgttatttagacgttgcagcaaatcataatattgacgtttctaatgtcgagcatcagttcccactgcttgtaaatttaaatcatgatcgtgatgttgctcatcaagaagcaacaacctatttagcaagttatattgccgaggtatatccgcatctaaatcagcaacaaaaaatggctgaacttattagccaacatgcgattggtactgataatgattactatgaatcaacattaaatgcgttagagcgtacaggttcaaaaaatgtattactttcttttgaatcaatgaaaaatcatgatgatgttgtaaacgtgattaatatggtcaatgagaaaattcaaaagaatttaccaagctcgtaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K216000_sequence 1 atgaattttggattattcttcctcaactttcagcctgaaaacacatcgtcagaaactgttttagataatatgatcaatatggtgtctctggttgataaagattataaaaactttacaactattttagtcaatgagcaccatttttctaaaaatggtattgtcggtgccccgatcactgctgcgagcttcctattagggttaactgaacgtttacacattggttctttaaatcaagttattacaactcatcacccggttcgtattgcagaagaagcaagtttacttgaccaaatgtcagatagtcgttttattctaggtttaagtgattgtattaatgattttgagatggatttctttaaacgtcagcgtgattcacagcagctacaatttgaagcttgttatgagatcattaatgaagcaatcacaaccaattattgccaagcgaataatgatttttataacttccctcgcatctcaattaatcctcattgcctgagtaaagagaatatgaagcaatatattttagcttctagtgtgagtgttgttgagtgggcggctaaaaaagcgctgccactgacatatcgttggagcgatagccttgaagataaagagattctttataagcgttatttagacgttgcagcaaatcataatattgacgtttctaatgtcgagcatcagttcccactgcttgtaaatttaaatcatgatcgtgatgttgctcatcaagaagcaacaacctatttagcaagttatattgccgaggtatatccgcatctaaatcagcaacaaaaaatggctgaacttattagccaacatgcgattggtactgataatgattactatgaatcaacattaaatgcgttagagcgtacaggttcaaaaaatgtattactttcttttgaatcaatgaaaaatcatgatgatgttgtaaacgtgattaatatggtcaatgagaaaattcaaaagaatttaccaagctcgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z