BBa_K216006 1 BBa_K216006 pentaerythritol tetranitrate reductase with native rbs 2009-10-15T11:00:00Z 2015-05-08T01:11:30Z This part was amplified from plasmid pONR1 which bears the PETN reductase gene originally cloned from ''Enterobacter cloacae'' PB2. This is the coding sequence for the enzyme pentaerythritol tetranitrate reductase (PETN reductase) of ''Enterobacter cloacae'' PB2. This enzyme reductively denitrates nitrate esters such as PETN (pentaerythritol tetranitrate, penthrite) GTN (glycerol trinitrate, nitroglycerine) and EGDN (ethylene glycol dinitrate) yielding the alcohol and nitrite ion. It can also reductively attack nitroaromatic compounds such as TNT (2,4,6-trinitrotoluene) forming initially hydride-Meisenheimer complexes which break down via an unknown route to yield nitrite and non-aromatic products. Reference: Binks, P.R., French, C.E., Nicklin, S., & Bruce, N.C. (1996). Degradation of pentaerythritol tetranitrate by ''Enterobacter cloacae'' PB2. Applied and Environmental Microbiology 62(4), 1214-1219. Reference 2: French,C.E., Nicklin,S. and Bruce,N.C. 1996. Sequence and properties of pentaerythritol tetranitrate reductase from ''Enterobacter cloacae'' PB2. J. Bacteriol. 178 (22), 6623-6627 (1996). false false _317_ 0 837 163 It's complicated true No special considerations, but due to an error, the primers were actually designed based on the homologous gene of another strain of ''Enterobacter cloacae'', which has a very similar coding sequence but different sequence in the upstream region. The sequence shown here is the correct sequence determined by sequencing the construct. false Edinburgh iGEM 2009 annotation2046804 1 PETN reductase coding region range2046804 1 9 1106 annotation2046803 1 stop range2046803 1 1104 1109 annotation2046801 1 RBS range2046801 1 1 3 annotation2046802 1 Start range2046802 1 9 11 BBa_K216006_sequence 1 gagtcgttatgtcagctgaaaagctgtttaccccactgaaagtgggtgccgttactgccccaaaccgcgtgtttatggccccacttacccgtctgcgcagcatcgagccgggcgatatcccaacgccattgatgggtgagtattaccgccagcgcgccagcgcgggcctgattatctccgaagccacgcagatttctgctcaggcaaaaggctacgccggtgcaccgggtctgcacagcccggaacagatcgccgcgtggaaaaaaatcaccgcaggcgtgcatgctgaagatggccgtattgcggttcagctgtggcacaccggtcgtatctcacacagcagcatccagcctggcggtcaggcgccggtttctgcctctgccctgaacgccaatacccgcacttccctgcgcgatgaaaacggtaatgcgatccgcgtcgacaccaccacgccacgcgcgctggagctggacgagatcccgggtatcgtgaatgatttccgtcaggccgtcgccaacgcccgggaagcgggcttcgacctggttgagcttcactctgcgcacggttacctgctgcatcagttcctgtccccgtcttccaaccagcgtaccgaccagtacggcggcagcgttgaaaaccgcgcgcgtctggtgcttgaagtggtggatgctgtctgtaatgagtggagcgcagaccgcattggtattcgtgtctccccgatcggtactttccagaacgtcgacaacggtccgaacgaagaagcagacgcgctgtatctgattgaagagctggcgaaacgcggtatcgcctatctgcacatgtccgagacggacttggcaggcggcaagccttacagtgaagccttccgtcagaaagtgcgcgagcgcttccacggcgtgattatcggggcgggtgcgtatacggcagaaaaagccgaggatttgatcggtaaaggcctgatcgacgccgtggcctttggccgtgactacattgctaacccggatctggttgcccgtttgcagaaaaaagccgaactgaacccgcagcgtcctgaaagcttctatggcggcggcgcggaaggttataccgactaccctacgctgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z