LOCUS BBa_K216015 1777 bp DNA linear 06-Nov-2024 DEFINITION PyeaR with firefly luciferase ACCESSION BBa_K216015 VERSION BBa_K216015.1 FEATURES Location/Qualifiers misc_feature 50..65 /label=NarL-binding region misc_feature 58..80 /label=NsrR-binding region misc_feature 66..71 /label=promoter -35 region misc_feature 89..94 /label=promoter -10 region promoter 1..100 /label=BBa_K216005 misc_feature 109..111 /label=SacI RBS 112..118 /label=rbs RBS 109..118 /label=BBa_J15001 CDS 125..1777 /label=BBa_I712019 ORIGIN 1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa 61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagct caaggaggta 121 ctagatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga 181 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac 241 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat 301 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt 361 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg 421 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg 481 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt 541 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca 601 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata 661 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc 721 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt 781 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag 841 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg 901 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga 961 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac 1021 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct 1081 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg 1141 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc 1201 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg 1261 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc 1321 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca 1381 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa 1441 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct 1501 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga 1561 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt 1621 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt 1681 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct 1741 cataaaggcc aagaagggcg gaaagatcgc cgtgtaa //