LOCUS       BBa_K216015             1777 bp    DNA     linear       22-Dec-2024
DEFINITION  PyeaR with firefly luciferase
ACCESSION   BBa_K216015
VERSION     BBa_K216015.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     misc_feature    109..111
                     /label=SacI
     RBS             112..118
                     /label=rbs
     RBS             109..118
                     /label=BBa_J15001
     CDS             125..1777
                     /label=BBa_I712019
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagct caaggaggta
      121 ctagatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
      181 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
      241 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
      301 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
      361 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
      421 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
      481 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
      541 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca
      601 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
      661 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc
      721 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt
      781 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
      841 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
      901 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga
      961 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac
     1021 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct
     1081 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg
     1141 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     1201 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     1261 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc
     1321 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     1381 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa
     1441 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct
     1501 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga
     1561 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     1621 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     1681 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     1741 cataaaggcc aagaagggcg gaaagatcgc cgtgtaa
//