LOCUS       BBa_K216016             2218 bp    DNA     linear       25-Nov-2024
DEFINITION  PyeaR with bacterial luciferase
ACCESSION   BBa_K216016
VERSION     BBa_K216016.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     RBS             123..128
                     /label=rbs
     misc_feature    135..137
                     /label=start luxA
     misc_feature    1215..1217
                     /label=end luxA
     CDS             135..1217
                     /label=luxA coding sequence
     RBS             1220..1225
                     /label=rbs
     misc_feature    1232..1234
                     /label=start luxB
     misc_feature    2213..2215
                     /label=end luxB
     CDS             1232..2215
                     /label=luxB coding sequence
     misc_feature    109..2218
                     /label=BBa_K216008
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagct caaatagcaa
      121 tataaggact ctctatgaaa tttggaaact ttttgcttac ataccaacct ccccaatttt
      181 ctcaaacaga ggtaatgaaa cgtttggtta aattaggtcg catctctgag gagtgtggtt
      241 ttgataccgt atggttactg gagcatcatt tcacggagtt tggtttgctt ggtaaccctt
      301 atgtcgctgc tgcatattta cttggcgcga ctaaaaaatt gaatgtagga actgccgcta
      361 ttgttcttcc cacagcccat ccagtacgcc aacttgaaga tgtgaattta ttggatcaaa
      421 tgtcaaaagg acgatttcgg tttggtattt gccgagggct ttacaacaag gactttcgcg
      481 tattcggcac agatatgaat aacagtcgcg ccttagcgga atgctggtac gggctgataa
      541 agaatggcat gacagaggga tatatggaag ctgataatga acatatcaag ttccataagg
      601 taaaagtaaa ccccgcggcg tatagcagag gtggcgcacc ggtttatgtg gtggctgaat
      661 cagcttcgac gactgagtgg gctgctcaat ttggcctacc gatgatatta agttggatta
      721 taaatactaa cgaaaagaaa gcacaacttg agctttataa tgaagtggct caagaatatg
      781 ggcacgatat tcataatatc gaccattgct tatcatatat aacatctgta gatcatgact
      841 caattaaagc gaaagagatt tgccggaaat ttctggggca ttggtatgat tcttatgtga
      901 atgctacgac tatttttgat gattcagacc aaacaagagg ttatgatttc aataaagggc
      961 agtggcgtga ctttgtatta aaaggacata aagatactaa tcgccgtatt gattacagtt
     1021 acgaaatcaa tcccgtggga acgccgcagg aatgtattga cataattcaa aaagacattg
     1081 atgctacagg aatatcaaat atttgttgtg gatttgaagc taatggaaca gtagacgaaa
     1141 ttattgcttc catgaagctc ttccagtctg atgtcatgcc atttcttaaa gaaaaacaac
     1201 gttcgctatt atattagcta aggagaaaga aatgaaattt ggattgttct tccttaactt
     1261 catcaattca acaactgttc aagaacaaag tatagttcgc atgcaggaaa taacggagta
     1321 tgttgataag ttgaattttg aacagatttt agtgtatgaa aatcattttt cagataatgg
     1381 tgttgtcggc gctcctctga ctgtttctgg ttttctgctc ggtttaacag agaaaattaa
     1441 aattggttca ttaaatcaca tcattacaac tcatcatcct gtcgccatag cggaggaagc
     1501 ttgcttattg gatcagttaa gtgaagggag atttatttta gggtttagtg attgcgaaaa
     1561 aaaagatgaa atgcattttt ttaatcgccc ggttgaatat caacagcaac tatttgaaga
     1621 gtgttatgaa atcattaacg atgctttaac aacaggctat tgtaatccag ataacgattt
     1681 ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg gacctcggaa
     1741 atatgtaaca gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag gtattcctct
     1801 catctttaag tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa gatataaagc
     1861 cgttgcggat aaatatgacg ttgacctatc agagatagac catcagttaa tgatattagt
     1921 taactataac gaagatagta ataaagctaa acaagagacg cgtgcattta ttagtgatta
     1981 tgttcttgaa atgcacccta atgaaaattt cgaaaataaa cttgaagaaa taattgcaga
     2041 aaacgctgtc ggaaattata cggagtgtat aactgcggct aagttggcaa ttgaaaagtg
     2101 tggtgcgaaa agtgtattgc tgtcctttga accaatgaat gatttgatga gccaaaaaaa
     2161 tgtaatcaat attgttgatg ataatattaa gaagtaccac atggaatata cctaataa
//