BBa_I712015 1 BBa_I712015 HIV-1 protease cleavage site 2007-10-20T11:00:00Z 2015-08-31T04:07:46Z This is proteolytic cleavage site for HIV-1 protease. false false _130_ 0 1883 9 In stock false true Katja Kolar BBa_E0020 1 ecfp engineered cyan fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_K592101 1 YFP Yellow Fluorescent Protein (YFP) 2011-09-20T11:00:00Z 2016-01-25T04:47:08Z Elowitz, M. B., A. J. Levine, et al. (2002). "Stochastic gene expression in a single cell." Science 297(5584): 1183-6. Yellow Fluorescent Protein derived from A. victoria GFP. This fluorescent protein is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original sequence was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington. false false _763_ 4206 9827 9 In stock false This fluorescent protein was cloned from the pZE12-YFP plasmid used by Elowitz (see reference). false Erik Lundin annotation2137506 1 YFP range2137506 1 1 714 BBa_K2163000 1 BBa_K2163000 Measurement of Protease Activity using FRET 2016-10-13T11:00:00Z 2016-10-14T02:38:24Z All the biobricks used come from the registry This part Consists of 3 biobricks, namely Cyan Fluorescent Protein, HIV Cleavage Site and Yellow Fluorescent Protein. The cleavage site is specific to HIV 1 Aspartyl Protease. The two proteins form a FRET pair. FRET stands for Fluorescence Resonance Energy Transfer. It is a phenomenon is which excitation of one protein leads to emission from the other, due to overlapping wavelengths. The two proteins need to be close together for this to take place. In our project we make use of this to measure the protease activity of HIV 1 Aspartyl Protease. false false _2634_ 26806 26806 9 false The design considerations include 1. Choice of the Fluorescence proteins 2. Distance between them false Rhushikesh Phadke component2501342 1 BBa_E0020 component2501343 1 BBa_I712015 component2501345 1 BBa_K592101 annotation2501342 1 BBa_E0020 range2501342 1 1 723 annotation2501343 1 BBa_I712015 range2501343 1 732 773 annotation2501345 1 BBa_K592101 range2501345 1 780 1499 BBa_K2163000_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagagccaggtgagccagaactaccccatcgtgcagaacctccaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtctaaaatgctttgctagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcctatcaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0020_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_I712015_sequence 1 agccaggtgagccagaactaccccatcgtgcagaacctccaa BBa_K592101_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtctaaaatgctttgctagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcctatcaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z