BBa_K2165001 1 BBa_K2165001 Violacein D gene codon-optimized for S. Cerevisiae 2016-10-13T11:00:00Z 2016-10-14T08:35:32Z ​VioC​ ​is​ ​originally​ ​from​ ​the​ violacein​ ​metabolic pathway​ ​in​ ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The original vioC sequence for the bacteria chassis was submitted by the Alberta team in 2013 as http://parts.igem.org/Part:BBa_K1161004 K1161004 and has been codon-optimized for use in yeast in this part. This​ ​part​ ​is​ ​a​ ​codon​ ​optimized​ ​version​ ​for​ ​yeast​ ​of​ ​the​ ​violacein​ ​D (vioD)​ ​gene​ ​submitted​ ​by​ ​the​ ​Alberta team​ ​in​ ​2013​ ​as​ ​http://parts.igem.org/Part:BBa_K1161004 K1161004 and is easy to insert in a custom violacein system.​ ​VioD ​is​ ​one​ ​of​ ​five​ ​genes​ ​in the violacein metabollic pathway that​ ​together​ ​produce​ ​the​ ​purple​ ​pigmented​ ​antibiotic, violacein,​ ​when​ ​expressed​ ​in​ ​yeast. false false _2636_ 28370 28370 9 false The online codon-optimization tool offered by Integrated DNA Technologies (https://www.idtdna.com/CodonOpt) was used to codon-optimize this sequence for use in yeast. Combinations containing RFC 10 illegal restriction sites were actively avoided. false Roya Amini-Naieni annotation2530877 1 VioD range2530877 1 1 1128 BBa_K2165001_sequence 1 atgaagatacttgtcataggagcgggtccggctgggctagtgttcgcgtcccagttgaaacaggcccgtccgctgtgggcaatcgacattgtcgagaaaaacgacgagcaagaggtattagggtggggcgtcgtgttgccagggaggccagggcaacaccctgcgaacccacttagttacttggacgctccggagaggctaaacccgcaattcctggaagactttaagctggtacaccacaacgagccaagtttaatgtctacgggggttcttctatgcggggtcgaaagacgtggcctagtacatgcgctaagggacaaatgcagatcacaaggcatcgcgattaggtttgaaagtcctttacttgagcacggagaattgccactagctgattatgacttagttgttcttgccaatggagttaatcacaagacggctcacttcacggaggcgctggttccacaagtggattatggtagaaacaagtacatttggtatggcaccagccagcttttcgatcaaatgaatctggtcttcagaacgcatggaaaggatatattcatcgctcatgcgtacaaatattccgataccatgtccacgttcattgttgagtgttctgaggagacctatgctcgtgctagattaggcgagatgagtgaggaagcctccgccgagtatgtcgcgaaggtctttcaagcggaactaggcggtcatggcttggtgtctcaaccgggtctgggctggcgtaactttatgacgctgagtcacgataggtgtcacgacgggaaattagtgttgttgggtgatgccctacaatcaggccacttctccatcggccacggaacgacgatggcggtagttgtggcacagttacttgtcaaggcattgtgtacggaggacggggtaccggcagcattgaaaaggtttgaggaacgtgcgttgcctctggtgcaactatttcgtggtcacgcggataacagccgtgtttggttcgagacggtagaagagcgtatgcatctttcaagtgcggaatttgtccagtcatttgatgctagaaggaagtctctgccgccaatgcccgaagccttggcacaaaacctgagatatgccttgcaacgtggcagctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z