BBa_K801012 1 BBa_K801012 ADH1 yeast terminator 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Extracted from genomic DNA of ''S. cerevisiae'' using PCR. Released HQ 2013 ADH1 terminator of ''S. cerevisiae'' false false _1057_ 0 13624 9 In stock false - false Georg Sch??tzinger annotation2202145 1 ADH1-Terminator range2202145 1 1 349 BBa_K2165000 1 BBa_K2165000 Violacein C gene codon-optimized for S. Cerevisiae 2016-10-13T11:00:00Z 2016-10-14T08:10:04Z ​VioC​ ​is​ ​originally​ ​from​ ​the​ violacein​ ​metabolic pathway​ ​in​ ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The original vioC sequence for the bacteria chassis was submitted by the Alberta team and has been codon-optimized for use in yeast in this part. This​ ​part​ ​is​ ​a​ ​codon​ ​optimized​ ​version​ ​for​ ​yeast​ ​of​ ​the​ ​violacein​ ​C (vioC)​ ​gene​ ​submitted​ ​by​ ​the​ ​Alberta team​ ​in​ ​2013​ ​as​ ​K1161003 and is easy to insert in a custom violacein system.​ ​VioC​ ​is​ ​one​ ​of​ ​five​ ​genes​ ​in the violacein metabollic pathway that​ ​together​ ​produce​ ​the​ ​purple​ ​pigmented​ ​antibiotic, violacein,​ ​when​ ​expressed​ ​in​ ​yeast. false false _2636_ 28370 28370 9 false The online codon-optimization tool offered by Integrated DNA Technologies (https://www.idtdna.com/CodonOpt) was used to codon-optimize this sequence for use in yeast. Combinations containing RFC 10 illegal restriction sites were actively avoided. false Roya Amini-Naieni annotation2530822 1 VioC range2530822 1 1 1296 BBa_K2165002 1 BBa_K2165002 Violacein C gene with CUP1 promoter + ADH1 terminator 2016-10-13T11:00:00Z 2016-10-15T11:06:25Z VioC is derived from the violacein pathway in ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in S. cerevisiae. The ADH1 terminator is also from the genomic DNA of S. cerevisiae. This part contains the VioC gene codon optimized for yeast (BBa_K2165000) attached to the CUP1 inducible yeast promoter (BBa_K2165004) and the ADH1 terminator. The inclusion of an inducible promoter in this construct makes it easier to control the color output of the violacein pathway by altering the concentration of cupric ions in the cell environment to control the level of expression in the presence of other genes in the violacein pathway. false false _2636_ 28370 28370 9 false The VioC gene was codon optimized for use in yeast to optimize response time of the violacein system due to an introduction of cupric ions. false Roya Amini-Naieni component2512644 1 BBa_K801012 component2512642 1 BBa_K2165000 component2512641 1 BBa_K2165004 annotation2512641 1 BBa_K2165004 range2512641 1 1 263 annotation2512644 1 BBa_K801012 range2512644 1 1574 1922 annotation2512642 1 BBa_K2165000 range2512642 1 270 1565 BBa_K2165004 1 BBa_K2165004 CUP1 yeast inducible promoter with RFC[10] restriction sites removed 2016-10-13T11:00:00Z 2016-10-14T09:20:00Z The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in Saccharomyces cerevisiae. CUP1 is a commonly used inducible transcription promoter for yeast that leads to enhanced expression of its respective gene(s) in the presence of cupric ions. The documented natural variants of the CUP1 promoter, including part K945002 in the registry (http://parts.igem.org/Part:BBa_K945002), contain RFC[10] illegal restriction sites that have been eliminated in this part so that it may be used in other biobricks. false false _2636_ 28370 28370 9 false The Yeast Promoter Atlas was used to prevent altering bases that served a significant regulatory purpose (http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=YHR053C#promoter_map). One base pair was substituted to eliminate the XbaI site in the middle of the sequence. false Roya Amini-Naieni annotation2531616 1 ChangedBase(GtoA) range2531616 1 84 84 annotation2530978 1 pCup1 range2530978 1 1 263 BBa_K801012_sequence 1 agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg BBa_K2165000_sequence 1 atgaaacgtgcaataattgttggagggggattagcaggtggcctaacagctatatacttagcaaagcgtggatacgaggtgcatgttgtagaaaaaaggggagacccgctgagagatttatcatcatatgtcgacgtcgtaagtagcagggcaatcggtgtatcaatgacagttagaggcataaagtctgttttggctgcggggattcccagggcggagcttgacgcttgtggagagcccattgttgctatggcatttagcgtgggtggtcagtaccgtatgcgtgaattgaaacctcttgaagacttcaggccgctttcactaaacagagcggcgttccagaaattacttaataagtacgcaaatcttgcgggggtgcgttattactttgagcataaatgtttagatgtagacctggacgggaaatctgtgttaatccaaggaaaggacgggcagccccaaaggctacaaggcgatatgatcattggggctgatggggcccactcagcggtacgtcaagctatgcaaagcgggctaagaaggttcgaatttcagcagaccttcttccgtcatgggtacaagacgctggtcttgcctgacgctcaggcgttggggtataggaaagacacactttatttttttggaatggacagtggaggattattcgcgggcagggccgctacaattccagacgggagtgtgagcatcgccgtttgtcttccttattctggatctcctagtttaaccacgacggacgagccgacaatgcgtgcattttttgacagatattttggggggctaccaagagatgcacgtgatgagatgctgcgtcagtttttagcgaagccgtcaaatgacttaatcaacgtaagatcaagtacgttccattataagggaaatgtgctattgttgggagacgcagcccacgcaaccgctccgtttttagggcaaggcatgaatatggcccttgaggatgcgagaacattcgtggagctgttggataggcatcagggcgaccaagataaagcttttccagagttcacagagctgcgtaaagtacaagccgatgcgatgcaggacatggcgcgtgcgaactacgatgtactgagctgtagtaaccccattttttttatgagggcaaggtacacgagatatatgcatagcaagtttcctggtctttatcccccagacatggccgaaaagctttacttcacctctgagccgtatgatcgtctacaacagattcagagaaagcagaatgtctggtataagattgggagagtcaatgggtcataa BBa_K2165004_sequence 1 aaaactagccttgttgctagttagaaaaagacatttttgctgtcagtcactgtcaagagattcttttgctggcatttcttctaaaagcaaaaagagcgatgcgtcttttccgctgaaccgttccagcaaaaaagactaccaacgcaatatggattgtcagaatcatataaaagagaagcaaataactccttgtcttgtatcaattgcattataatatcttcttgttagtgcaatatcatatagaagtcatcgaaatagatatt BBa_K2165002_sequence 1 aaaactagccttgttgctagttagaaaaagacatttttgctgtcagtcactgtcaagagattcttttgctggcatttcttctaaaagcaaaaagagcgatgcgtcttttccgctgaaccgttccagcaaaaaagactaccaacgcaatatggattgtcagaatcatataaaagagaagcaaataactccttgtcttgtatcaattgcattataatatcttcttgttagtgcaatatcatatagaagtcatcgaaatagatatttactagatgaaacgtgcaataattgttggagggggattagcaggtggcctaacagctatatacttagcaaagcgtggatacgaggtgcatgttgtagaaaaaaggggagacccgctgagagatttatcatcatatgtcgacgtcgtaagtagcagggcaatcggtgtatcaatgacagttagaggcataaagtctgttttggctgcggggattcccagggcggagcttgacgcttgtggagagcccattgttgctatggcatttagcgtgggtggtcagtaccgtatgcgtgaattgaaacctcttgaagacttcaggccgctttcactaaacagagcggcgttccagaaattacttaataagtacgcaaatcttgcgggggtgcgttattactttgagcataaatgtttagatgtagacctggacgggaaatctgtgttaatccaaggaaaggacgggcagccccaaaggctacaaggcgatatgatcattggggctgatggggcccactcagcggtacgtcaagctatgcaaagcgggctaagaaggttcgaatttcagcagaccttcttccgtcatgggtacaagacgctggtcttgcctgacgctcaggcgttggggtataggaaagacacactttatttttttggaatggacagtggaggattattcgcgggcagggccgctacaattccagacgggagtgtgagcatcgccgtttgtcttccttattctggatctcctagtttaaccacgacggacgagccgacaatgcgtgcattttttgacagatattttggggggctaccaagagatgcacgtgatgagatgctgcgtcagtttttagcgaagccgtcaaatgacttaatcaacgtaagatcaagtacgttccattataagggaaatgtgctattgttgggagacgcagcccacgcaaccgctccgtttttagggcaaggcatgaatatggcccttgaggatgcgagaacattcgtggagctgttggataggcatcagggcgaccaagataaagcttttccagagttcacagagctgcgtaaagtacaagccgatgcgatgcaggacatggcgcgtgcgaactacgatgtactgagctgtagtaaccccattttttttatgagggcaaggtacacgagatatatgcatagcaagtttcctggtctttatcccccagacatggccgaaaagctttacttcacctctgagccgtatgatcgtctacaacagattcagagaaagcagaatgtctggtataagattgggagagtcaatgggtcataatactagagagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z