BBa_K2165003 1 BBa_K2165003 Violacein D gene with Gal1 promoter + ADH1 terminator 2016-10-13T11:00:00Z 2016-10-15T11:07:18Z VioD is derived from the violacein pathway in ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The Gal1 promoter is from the genomic DNA of S. cerevisiae in the GAL1 locus. The ADH1 terminator is also from the genomic DNA of S. cerevisiae. This part contains the VioD gene codon optimized for yeast (BBa_K2165001) attached to the Gal1 inducible yeast promoter (BBa_J63006) and the ADH1 terminator. The inclusion of an inducible promoter in this construct makes it easier to control the color output of the violacein pathway by altering the concentration of galactose in the cell environment to control the level of expression in the presence of other genes in the violacein pathway. false false _2636_ 28370 28370 9 false The VioD gene was codon optimized for use in yeast to optimize response time of the violacein system due to an introduction of galactose. false Roya Amini-Naieni component2512646 1 BBa_K2165001 component2512648 1 BBa_K801012 component2512645 1 BBa_J63006 annotation2512646 1 BBa_K2165001 range2512646 1 556 1683 annotation2512645 1 BBa_J63006 range2512645 1 1 549 annotation2512648 1 BBa_K801012 range2512648 1 1692 2040 BBa_K2165001 1 BBa_K2165001 Violacein D gene codon-optimized for S. Cerevisiae 2016-10-13T11:00:00Z 2016-10-14T08:35:32Z ​VioC​ ​is​ ​originally​ ​from​ ​the​ violacein​ ​metabolic pathway​ ​in​ ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The original vioC sequence for the bacteria chassis was submitted by the Alberta team in 2013 as http://parts.igem.org/Part:BBa_K1161004 K1161004 and has been codon-optimized for use in yeast in this part. This​ ​part​ ​is​ ​a​ ​codon​ ​optimized​ ​version​ ​for​ ​yeast​ ​of​ ​the​ ​violacein​ ​D (vioD)​ ​gene​ ​submitted​ ​by​ ​the​ ​Alberta team​ ​in​ ​2013​ ​as​ ​http://parts.igem.org/Part:BBa_K1161004 K1161004 and is easy to insert in a custom violacein system.​ ​VioD ​is​ ​one​ ​of​ ​five​ ​genes​ ​in the violacein metabollic pathway that​ ​together​ ​produce​ ​the​ ​purple​ ​pigmented​ ​antibiotic, violacein,​ ​when​ ​expressed​ ​in​ ​yeast. false false _2636_ 28370 28370 9 false The online codon-optimization tool offered by Integrated DNA Technologies (https://www.idtdna.com/CodonOpt) was used to codon-optimize this sequence for use in yeast. Combinations containing RFC 10 illegal restriction sites were actively avoided. false Roya Amini-Naieni annotation2530877 1 VioD range2530877 1 1 1128 BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_K801012 1 BBa_K801012 ADH1 yeast terminator 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Extracted from genomic DNA of ''S. cerevisiae'' using PCR. Released HQ 2013 ADH1 terminator of ''S. cerevisiae'' false false _1057_ 0 13624 9 In stock false - false Georg Sch??tzinger annotation2202145 1 ADH1-Terminator range2202145 1 1 349 BBa_K801012_sequence 1 agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_K2165003_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatgaagatacttgtcataggagcgggtccggctgggctagtgttcgcgtcccagttgaaacaggcccgtccgctgtgggcaatcgacattgtcgagaaaaacgacgagcaagaggtattagggtggggcgtcgtgttgccagggaggccagggcaacaccctgcgaacccacttagttacttggacgctccggagaggctaaacccgcaattcctggaagactttaagctggtacaccacaacgagccaagtttaatgtctacgggggttcttctatgcggggtcgaaagacgtggcctagtacatgcgctaagggacaaatgcagatcacaaggcatcgcgattaggtttgaaagtcctttacttgagcacggagaattgccactagctgattatgacttagttgttcttgccaatggagttaatcacaagacggctcacttcacggaggcgctggttccacaagtggattatggtagaaacaagtacatttggtatggcaccagccagcttttcgatcaaatgaatctggtcttcagaacgcatggaaaggatatattcatcgctcatgcgtacaaatattccgataccatgtccacgttcattgttgagtgttctgaggagacctatgctcgtgctagattaggcgagatgagtgaggaagcctccgccgagtatgtcgcgaaggtctttcaagcggaactaggcggtcatggcttggtgtctcaaccgggtctgggctggcgtaactttatgacgctgagtcacgataggtgtcacgacgggaaattagtgttgttgggtgatgccctacaatcaggccacttctccatcggccacggaacgacgatggcggtagttgtggcacagttacttgtcaaggcattgtgtacggaggacggggtaccggcagcattgaaaaggtttgaggaacgtgcgttgcctctggtgcaactatttcgtggtcacgcggataacagccgtgtttggttcgagacggtagaagagcgtatgcatctttcaagtgcggaatttgtccagtcatttgatgctagaaggaagtctctgccgccaatgcccgaagccttggcacaaaacctgagatatgccttgcaacgtggcagctaatactagagagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg BBa_K2165001_sequence 1 atgaagatacttgtcataggagcgggtccggctgggctagtgttcgcgtcccagttgaaacaggcccgtccgctgtgggcaatcgacattgtcgagaaaaacgacgagcaagaggtattagggtggggcgtcgtgttgccagggaggccagggcaacaccctgcgaacccacttagttacttggacgctccggagaggctaaacccgcaattcctggaagactttaagctggtacaccacaacgagccaagtttaatgtctacgggggttcttctatgcggggtcgaaagacgtggcctagtacatgcgctaagggacaaatgcagatcacaaggcatcgcgattaggtttgaaagtcctttacttgagcacggagaattgccactagctgattatgacttagttgttcttgccaatggagttaatcacaagacggctcacttcacggaggcgctggttccacaagtggattatggtagaaacaagtacatttggtatggcaccagccagcttttcgatcaaatgaatctggtcttcagaacgcatggaaaggatatattcatcgctcatgcgtacaaatattccgataccatgtccacgttcattgttgagtgttctgaggagacctatgctcgtgctagattaggcgagatgagtgaggaagcctccgccgagtatgtcgcgaaggtctttcaagcggaactaggcggtcatggcttggtgtctcaaccgggtctgggctggcgtaactttatgacgctgagtcacgataggtgtcacgacgggaaattagtgttgttgggtgatgccctacaatcaggccacttctccatcggccacggaacgacgatggcggtagttgtggcacagttacttgtcaaggcattgtgtacggaggacggggtaccggcagcattgaaaaggtttgaggaacgtgcgttgcctctggtgcaactatttcgtggtcacgcggataacagccgtgtttggttcgagacggtagaagagcgtatgcatctttcaagtgcggaatttgtccagtcatttgatgctagaaggaagtctctgccgccaatgcccgaagccttggcacaaaacctgagatatgccttgcaacgtggcagctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z