LOCUS BBa_K2168001 5258 bp DNA linear 22-Nov-2024 DEFINITION pBAD/araC - (N-6xHis) - Ag43 - sfGFP ACCESSION BBa_K2168001 VERSION BBa_K2168001.1 FEATURES Location/Qualifiers CDS 1..879 /label=araC promoter 906..1190 /label=pBAD RBS 1219..1230 /label=B0034 CDS 1237..4371 /label=Ag43(PstI sites removed) misc_feature 4372..4401 /label=(GGGGS)2 linker CDS 4402..5118 /label=sfGFP misc_feature 5130..5258 /label=Double terminator (B0015) misc_feature 1396..1413 /label=6xHis ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc 1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc 1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc 1381 ccggtgctgg ctgctcatca tcaccatcac cacgacatcg ttgtgcaccc gggagaaacc 1441 gtgaacggcg gaacactggc aaatcatgac aaccagattg tcttcggtac gaccaacgga 1501 atgaccatca gtaccgggct ggagtatggg ccggataacg aggccaatac cggcgggcaa 1561 tgggtacagg atggcggaac agccaacaaa acgactgtca ccagtggtgg tcttcagaga 1621 gtgaaccccg gtggaagtgt ctcagacacg gttatcagtg ccggaggcgg acagagcctt 1681 cagggacggg ctgtgaacac cacgctgaat ggtggcgaac agtggatgca tgagggggcg 1741 atagccacag gaaccgtcat taatgataag ggctggcagg tcgtcaagcc cggtacagtg 1801 gcaacggata ccgttgttaa taccggggcg gaagggggac cggatgcaga aaacggtgat 1861 accgggcagt ttgttcgcgg ggatgccgta cgcacaacca tcaataaaaa cggtcgccag 1921 attgtgagag ctgaaggaac ggcaaatacc actgtggttt atgccggcgg cgaccagact 1981 gtacatggtc acgcactgga taccacgctg aatgggggat accagtatgt gcacaacggc 2041 ggtacagcgt ctgacactgt tgtgaacagt gacggctggc agattgtcaa aaacgggggt 2101 gtggccggga ataccaccgt taatcagaag ggcagactgc aagtggacgc cggtggtaca 2161 gccacgaatg tcaccctgaa gcagggcggc gcactggtta ccagtacggc tgcaaccgtt 2221 accggcataa accgcctggg agcattctct gttgtggagg gtaaagctga taatgtcgta 2281 ctggaaaatg gcggacgcct ggatgtgctg accggacaca cagccactaa tacccgcgtg 2341 gatgatggcg gaacgctgga tgtccgcaac ggtggcaccg ccaccaccgt atccatggga 2401 aatggcggtg tactgctggc cgattccggt gccgctgtca gtggtacccg gagcgacgga 2461 aaggcattca gtatcggagg cggtcaggcg gatgccctga tgctggaaaa aggcagttca 2521 ttcacgctga acgccggtga tacggccacg gataccacgg taaatggcgg actgttcacc 2581 gccaggggcg gcacactggc gggcaccacc acgctgaata acggcgccat acttaccctt 2641 tccgggaaga cggtgaacaa cgataccctg accatccgtg aaggcgatgc actcctgcaa 2701 ggaggctctc tcaccggtaa cggcagcgtg gaaaaatcag gaagtggcac actcactgtc 2761 agcaacacca cactcaccca gaaagccgtc aacctgaatg aaggcacgct gacgctgaac 2821 gacagtaccg tcaccacgga tgtcattgct cagcgcggta cagccctgaa gctgaccggc 2881 agcactgtgc tgaacggtgc cattgacccc acgaatgtca ctctcgcctc cggtgccacc 2941 tggaatatcc ccgataacgc cacggtgcag tcggtggtgg atgacctcag ccatgccgga 3001 cagattcatt tcacctccac ccgcacaggg aagttcgtac cggcaaccct gaaagtgaaa 3061 aacctgaacg gacagaatgg caccatcagc ctgcgtgtac gcccggatat ggcacagaac 3121 aatgctgaca gactggtcat tgacggcggc agggcaaccg gaaaaaccat cctgaacctg 3181 gtgaacgccg gcaacagtgc gtcggggctg gcgaccagcg gtaagggtat tcaggtggtg 3241 gaagccatta acggtgccac cacggaggaa ggggcctttg tccaggggaa caggctgcaa 3301 gccggtgcct ttaactactc cctcaaccgg gacagtgatg agagctggta tctgcgcagt 3361 gaaaatgctt atcgtgcaga agtccccctg tatgcctcca tgctgacaca ggcaatggac 3421 tatgaccgga ttgtggcagg ctcccgcagc catcagaccg gtgtaaatgg tgaaaacaac 3481 agcgtccgtc tcagcattca gggcggtcat ctcggtcacg ataacaatgg cggtattgcc 3541 cgtggggcca cgccggaaag cagcggcagc tatggattcg tccgtctgga gggtgacctg 3601 atgagaacag aggttgccgg tatgtctgtg accgcggggg tatatggtgc tgctggccat 3661 tcttccgttg atgttaagga tgatgacggc tcccgtgccg gcacggtccg ggatgatgcc 3721 ggcagcctgg gcggatacct gaatctggta cacacgtcct ccggcctgtg ggctgacatt 3781 gtggcacagg gaacccgcca cagcatgaaa gcgccatcgg acaataacga cttccgcgcc 3841 cggggctggg gctggctggg ctcactggaa accggtctgc ccttcagtat cactgacaac 3901 ctgatgctgg agccacaact gcaatatacc tggcagggac tttccctgga tgacggtaag 3961 gacaacgccg gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg tgccggtttc 4021 cgtctgggca gccacaacga tatgaccttt ggcgaaggca cctcatcccg tgcccccctg 4081 cgtgacagtg caaaacacag tgtgagtgaa ttaccggtga actggtgggt acagccttct 4141 gttatccgca ccttcagctc ccggggagat atgcgtgtgg ggacttccac tgcgggcagc 4201 gggatgacgt tctctccctc acagaatggc acatcactgg acctgcaagc cggactggaa 4261 gcccgtgtcc gggaaaatat caccctgggc gttcaggccg gttatgccca cagcgtcagc 4321 ggcagcagcg ctgaagggta taacggtcag gccacactga atgtgacctt cggtggtgga 4381 ggttcaggtg gtggtggctc gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct 4441 attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt 4501 gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg 4561 ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt 4621 tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg 4681 caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa 4741 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac 4801 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc 4861 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat 4921 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt 4981 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag 5041 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg 5101 gatgaactgt acaaatgatg atactagagc caggcatcaa ataaaacgaa aggctcagtc 5161 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 5221 cactggctca ccttcgggtg ggcctttctg cgtttata //