BBa_K2169001 1 BBa_K2169001 ZnO binding Curli protein 2016-10-13T11:00:00Z 2016-10-28T01:03:02Z CsgA: E. coli genome/ parts registry Curli subunit CsgA modified with a ZnO binding tag to enable binding of ZnO surfaces. false false _2640_ 25347 25347 9 false CsgA is secreted. In order not to interfere with the secretion signal, we had a look at CsgA fusion proteins. C-terminal fusions have been shown to be functional. For cloning into our expression vector with EcoRI, we had to add an RBS before the start codon. false Filip Cierniak component2509143 1 BBa_K2169137 component2509141 1 BBa_K2169139 component2509137 1 BBa_K1650034 component2509139 1 BBa_K1650041 annotation2509143 1 BBa_K2169137 range2509143 1 509 580 annotation2509139 1 BBa_K1650041 range2509139 1 19 471 annotation2509137 1 BBa_K1650034 range2509137 1 1 12 annotation2509141 1 BBa_K2169139 range2509141 1 480 500 BBa_K2169139 1 BBa_K2169139 GS7 Linker 2016-10-13T11:00:00Z 2016-10-14T01:49:23Z AA sequence from BBa_J18921 extended by one glycin. Linker sequence translating to gsgsgsg. false false _2640_ 25347 25347 9 false 7 feels better than 6. The linker's lenght was chosen arbitrarily. false Filip Cierniak annotation2509106 1 GS7 Linker range2509106 1 1 21 BBa_K2169137 1 BBa_K2169137 ZnO binding Tag 2016-10-13T11:00:00Z 2016-10-14T12:30:27Z Kj??rgaard et al., 2000 PMID:PMC91778, library of random peptides A peptide tag capable of binding to Zinc oxide surfaces. false false _2640_ 25347 25347 9 false DNA Sequence derived from published AA sequence and codon optimized for E. coli K12 using IDT Codon Optimizer. false Filip Cierniak annotation2508625 1 ZnO binding Tag range2508625 1 1 72 BBa_K1650034 1 BBa_K1650034 RBS 2015-09-17T11:00:00Z 2015-09-18T10:59:15Z test (T5) false false _2067_ 12520 27246 9 false test false Alexandra Richter annotation2474905 1 BBa_K1650034 range2474905 1 1 12 BBa_K1650041 1 BBa_K1650041 CsgA 2015-09-17T11:00:00Z 2015-09-18T11:10:05Z test Curli-Protein false false _2067_ 12520 27246 9 false test false Alexandra Richter annotation2474935 1 BBa_K1650041 range2474935 1 1 453 BBa_K2169137_sequence 1 cgcagtaatacccgcatgaccgcccgtcaacatcgctcagcgaatcataagtccacacaacgcgcccgcagt BBa_K2169139_sequence 1 ggatctggttcaggctccgga BBa_K1650034_sequence 1 aaggaggaatta BBa_K1650041_sequence 1 atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtatggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgctgttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtac BBa_K2169001_sequence 1 aaggaggaattatactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtatggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgctgttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactactagagggatctggttcaggctccggatactagagcgcagtaatacccgcatgaccgcccgtcaacatcgctcagcgaatcataagtccacacaacgcgcccgcagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z