BBa_K217000 1 BBa_K217000 cmv_tat_GFP 2009-10-19T11:00:00Z 2015-05-08T01:11:30Z The source of this part is our own version of a sequence received from Dr. Gao from Columbia University. The part is controlled by a cmv promotor. This makes it constituitivley active, or always on. It is a tat peptide sequence connected to GFP to create a fusion protein. Tat is a cell penetrating peptide that was first discovered by two independent labs studying the HIV-1. This fusion protein should be handled with a safety level 2 because of its ability to enter a cell it is considered infectious. Essentially, the cell that has this plasmid is able to shoot out green bullets. There will be reinsertion into the original source. false false _319_ 0 3208 9 It's complicated true No issues. The tat peptide can be directly attached to the GFP. false Jessamine Osborne BBa_K217000_sequence 1 aagcttatggctagcatgactggtggacagcaaatgggtcgggatctgtacgacgatgacgataaggatccccccgctgaattcatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatttaacaacggttacggtcgtaagaagcgtcgtcagcgtcgtcgtggctaagcggccgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z