BBa_K217000
1
BBa_K217000
cmv_tat_GFP
2009-10-19T11:00:00Z
2015-05-08T01:11:30Z
The source of this part is our own version of a sequence received from Dr. Gao from Columbia University.
The part is controlled by a cmv promotor. This makes it constituitivley active, or always on. It is a tat peptide sequence connected to GFP to create a fusion protein. Tat is a cell penetrating peptide that was first discovered by two independent labs studying the HIV-1. This fusion protein should be handled with a safety level 2 because of its ability to enter a cell it is considered infectious. Essentially, the cell that has this plasmid is able to shoot out green bullets. There will be reinsertion into the original source.
false
false
_319_
0
3208
9
It's complicated
true
No issues. The tat peptide can be directly attached to the GFP.
false
Jessamine Osborne
BBa_K217000_sequence
1
aagcttatggctagcatgactggtggacagcaaatgggtcgggatctgtacgacgatgacgataaggatccccccgctgaattcatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatttaacaacggttacggtcgtaagaagcgtcgtcagcgtcgtcgtggctaagcggccgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z