LOCUS BBa_K2170000 4171 bp DNA linear 26-Nov-2024 DEFINITION Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA ACCESSION BBa_K2170000 VERSION BBa_K2170000.1 FEATURES Location/Qualifiers promoter 1..559 /label=CMV promoter 604..622 /label=T7 misc_feature 632..643 /label=extended Kozak sequence CDS 641..691 /label=BM40 signal peptide misc_feature 701..745 /label=BAP CDS 752..1261 /label=NanoLuc misc_feature 1268..1300 /label=A3C5 Tag CDS 1307..1336 /label=poly G linker CDS 1343..1411 /label=EGFR TMD CDS 1412..1438 /label=EGFR stop transfer sequence CDS 1439..1468 /label=poly G linker CDS 1475..2182 /label=mRuby3 misc_feature 2189..2212 /label=Strep TagII RBS 2258..2597 /label=IRES (elF4G1) misc_feature 2604..2616 /label=extended Kozak sequence CDS 2611..2672 /label=Ig Kappa signal peptide CDS 2682..3641 /label=BirA misc_feature 3648..3672 /label=Strep TagII CDS 3648..3680 /label=StepDEL CDS 3670..3680 /label=KDEL (ER-retention signal polyA_site 3691..4171 /label=polyA ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagaact cactataggg 601 acttaatacg actcactata gggactagac cgccgccacc atgcgggcct ggatcttctt 661 cctgctgtgc ctggctggca gagccctggc tgccaccggc ggcctgaacg acatcttcga 721 ggcccagaaa atcgagtggc acgagaccgg cgtgttcacc ctcgaggatt tcgttggaga 781 ttggagacag accgctggat acaaccttga tcaggttttg gagcagggtg gtgtgtctag 841 tcttttccag aacctcggag tgtctgtgac ccctatccag agaatcgttc tctctggtga 901 gaacggactc aagatcgata tccacgtgat catcccttac gagggactct caggtgatca 961 gatgggacag atcgagaaga ttttcaaggt ggtgtaccct gttgatgatc accacttcaa 1021 ggtgatcctc cactacggaa ctctcgtgat cgatggtgtg accccaaaca tgatcgatta 1081 cttcggaagg ccatacgagg gaatcgctgt gttcgatgga aagaagatta ccgttaccgg 1141 aaccctctgg aacggaaaca agattatcga tgagaggctc atcaaccctg atggatctct 1201 cttgttcagg gtgaccatca acggtgtgac tggttggaga ctttgcgaga gaatccttgc 1261 taccggcttc gacaaaaact acgtagctaa ctctggtaag accggcggag gcggaggatc 1321 tggcggaggc ggatctcctt ctatcgccac cggaatggtg ggagccctcc tgctgctgct 1381 ggtggtggct ctgggcatcg gcctgttcat gcggcggaga cacatcgtgc ggaagagagg 1441 cggcggagga agcggagggg gcggatctac cggcgtgagc aaaggtgaag aactgatcaa 1501 agaaaacatg cggatgaaag ttgttatgga aggtagcgtt aatggccacc agtttaaatg 1561 tactggtgaa ggtgaaggtc gtccgtatga aggtgttcag gttatgcgta ttaaagtgat 1621 tgaaggtggt ccgctgccgt ttgcatttga tattctggca accagcttta tgtatggtag 1681 ccgtaccttt atcaaatatc cggcagatat tccggacttc tttaaacaga gctttccgga 1741 aggttttacc tgggaacgtg ttacccgtta tgaagatggt ggtgttgtta ccgttaccca 1801 ggataccagc ctggaagatg gcgaactggt ttataatgtt aaagtgcgtg gtgtgaattt 1861 tccgagcaat ggtccggtta tgcagaaaaa aaccaaaggt tgggaaccga ataccgaaat 1921 gatgtatcct gccgatggtg gtctgcgtgg ttataccgat attgcactga aagttgatgg 1981 tggcggtcat ctgcattgta attttgttac cacctaccgc tcaaaaaaaa ccgtgggtaa 2041 cattaaaatg cctggtgttc atgcagttga tcatcgtctg gaacgtattg aagaaagcga 2101 taatgaaacc tatgttgtgc agcgtgaagt tgcagttgca aaatatagca atctgggtgg 2161 tggtatggat gagctgtata aaaccggctg gtcccacccc cagttcgaga agtaatacta 2221 gagtagtgat cagcctcgac tgtgccttca cgtagtgaga tgggggtcct gggccccagg 2281 gtgtgcagcc actgacttgg ggactgctgg tggggtaggg atgagggagg gaggggcatt 2341 gtgatgtaca gggctgctct gtgagatcaa gggtctctta agggtgggag ctggggcagg 2401 gactacgaga gcagccagat gggctgaaag tggaactcaa ggggtttctg gcacctacct 2461 acctgcttcc cgctgggggg tggggagttg gcccagagtc ttaagattgg ggcagggtgg 2521 agaggtgggc tcttcctgct tcccactcat cttatagctt tctttcccca gatccgaatt 2581 ggagatccaa accaagggat ccagccgcca ccatggaaac cgacaccctg ctgctgtggg 2641 tgctgctgct gtgggtgcca ggatctacag gcgacggcgc caaggacaac accgtgcccc 2701 tgaagctgat tgccctgctg gccaacggcg agttccactc tggcgagcag ctgggcgaga 2761 cactgggcat gtcccgtgcc gccatcaaca agcacatcca gaccctgcgt gactggggcg 2821 tggacgtgtt caccgtgcct ggcaagggct acagcctgcc cgagcctatc cagctgctga 2881 acgccaagca gatcctgggc cagctggatg gcggatctgt ggctgtgctg cccgtgatcg 2941 acagcaccaa ccagtacctg ctggaccgga tcggcgagct gaaaagcggc gacgcctgta 3001 tcgccgagta tcagcaggct ggccgtggcc gtcggggccg taagtggttt agccccttcg 3061 gcgcaaatct gtacctgagc atgttctggc ggctggaaca gggacctgcc gccgctattg 3121 gactgagcct cgtgatcggc atcgtgatgg ccgaggtgct gcgtaagctg ggtgccgaca 3181 aagtgcgcgt gaagtggccc aacgacctgt atctgcaaga ccgtaagctg gcaggcatcc 3241 tggtggaact gacaggcaaa actggcgacg ccgcccagat cgtgattggc gctggcatca 3301 atatggccat gcgtcgggtg gaagagtccg tcgtgaatca gggctggatc accctgcaag 3361 aagccggtat caacctggac cgtaacaccc tggccgccat gctgatccgt gagctgcgtg 3421 ccgccctgga actgttcgag caggaaggcc tggcccccta cctgtcccgt tgggagaagc 3481 tggacaactt catcaaccgt cccgtgaaac tgatcatcgg cgacaaagag atcttcggca 3541 tcagccgggg catcgacaag cagggcgctc tgctgctgga acaggacggc atcatcaagc 3601 cctggatggg cggcgagatc agcctgcgtt ctgccgagaa aggtgcctgg tcccaccccc 3661 agttcgagaa ggatgagctg taatactaga gacgggtggc atccctgtga cccctcccca 3721 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa 3781 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg 3841 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg 3901 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt 3961 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg 4021 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc 4081 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag 4141 gcgtgaacca ctgctccctt ccctgtcctt t //