##gff-version 3
##sequence-region BBa_K2170000 1 4171
BBa_K2170000	.	CDS	1475	2182	.	+	0	ID=annotation2513211;Name=mRuby3
BBa_K2170000	.	CDS	2682	3641	.	+	0	ID=annotation2513216;Name=BirA
BBa_K2170000	.	CDS	641	691	.	+	0	ID=annotation2513203;Name=BM40 signal peptide
BBa_K2170000	.	polyA_site	3691	4171	.	+	0	ID=annotation2513255;Name=polyA
BBa_K2170000	.	ribosome_entry_site	2258	2597	.	+	0	ID=annotation2513213;Name=IRES (elF4G1)
BBa_K2170000	.	sequence_feature	2604	2616	.	+	0	ID=annotation2513214;Name=extended Kozak sequence
BBa_K2170000	.	promoter	1	559	.	+	0	ID=annotation2513200;Name=CMV
BBa_K2170000	.	CDS	1439	1468	.	+	0	ID=annotation2513210;Name=poly G linker
BBa_K2170000	.	CDS	1307	1336	.	+	0	ID=annotation2513207;Name=poly G linker
BBa_K2170000	.	sequence_feature	632	643	.	+	0	ID=annotation2513202;Name=extended Kozak sequence
BBa_K2170000	.	tag	3648	3672	.	+	0	ID=annotation2513217;Name=Strep TagII
BBa_K2170000	.	CDS	2611	2672	.	+	0	ID=annotation2513215;Name=Ig Kappa signal peptide
BBa_K2170000	.	tag	2189	2212	.	+	0	ID=annotation2513212;Name=Strep TagII
BBa_K2170000	.	CDS	3670	3680	.	+	0	ID=annotation2513219;Name=KDEL (ER-retention signal
BBa_K2170000	.	promoter	604	622	.	+	0	ID=annotation2513201;Name=T7
BBa_K2170000	.	CDS	1343	1411	.	+	0	ID=annotation2513208;Name=EGFR TMD
BBa_K2170000	.	tag	1268	1300	.	+	0	ID=annotation2513206;Name=A3C5 Tag
BBa_K2170000	.	CDS	752	1261	.	+	0	ID=annotation2513205;Name=NanoLuc
BBa_K2170000	.	non_covalent_binding_site	701	745	.	+	0	ID=annotation2513204;Name=BAP
BBa_K2170000	.	CDS	3648	3680	.	+	0	ID=annotation2513218;Name=StepDEL
BBa_K2170000	.	CDS	1412	1438	.	+	0	ID=annotation2513209;Name=EGFR stop transfer sequence
>BBa_K2170000
atagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccg
cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactt
tccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtac
gccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctact
tggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagc
ggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacggga
ctttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagc
agagctggtttagtgaaccgtcagatctactagaactcactatagggacttaatacgactcactatagggactagaccg
ccgccaccatgcgggcctggatcttcttcctgctgtgcctggctggcagagccctggctgccaccggcggcctgaacga
catcttcgaggcccagaaaatcgagtggcacgagaccggcgtgttcaccctcgaggatttcgttggagattggagacag
accgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtga
cccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggact
ctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatc
ctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcg
ctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaa
ccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgctacc
ggcttcgacaaaaactacgtagctaactctggtaagaccggcggaggcggaggatctggcggaggcggatctccttcta
tcgccaccggaatggtgggagccctcctgctgctgctggtggtggctctgggcatcggcctgttcatgcggcggagaca
catcgtgcggaagagaggcggcggaggaagcggagggggcggatctaccggcgtgagcaaaggtgaagaactgatcaaa
gaaaacatgcggatgaaagttgttatggaaggtagcgttaatggccaccagtttaaatgtactggtgaaggtgaaggtc
gtccgtatgaaggtgttcaggttatgcgtattaaagtgattgaaggtggtccgctgccgtttgcatttgatattctggc
aaccagctttatgtatggtagccgtacctttatcaaatatccggcagatattccggacttctttaaacagagctttccg
gaaggttttacctgggaacgtgttacccgttatgaagatggtggtgttgttaccgttacccaggataccagcctggaag
atggcgaactggtttataatgttaaagtgcgtggtgtgaattttccgagcaatggtccggttatgcagaaaaaaaccaa
aggttgggaaccgaataccgaaatgatgtatcctgccgatggtggtctgcgtggttataccgatattgcactgaaagtt
gatggtggcggtcatctgcattgtaattttgttaccacctaccgctcaaaaaaaaccgtgggtaacattaaaatgcctg
gtgttcatgcagttgatcatcgtctggaacgtattgaagaaagcgataatgaaacctatgttgtgcagcgtgaagttgc
agttgcaaaatatagcaatctgggtggtggtatggatgagctgtataaaaccggctggtcccacccccagttcgagaag
taatactagagtagtgatcagcctcgactgtgccttcacgtagtgagatgggggtcctgggccccagggtgtgcagcca
ctgacttggggactgctggtggggtagggatgagggagggaggggcattgtgatgtacagggctgctctgtgagatcaa
gggtctcttaagggtgggagctggggcagggactacgagagcagccagatgggctgaaagtggaactcaaggggtttct
ggcacctacctacctgcttcccgctggggggtggggagttggcccagagtcttaagattggggcagggtggagaggtgg
gctcttcctgcttcccactcatcttatagctttctttccccagatccgaattggagatccaaaccaagggatccagccg
ccaccatggaaaccgacaccctgctgctgtgggtgctgctgctgtgggtgccaggatctacaggcgacggcgccaagga
caacaccgtgcccctgaagctgattgccctgctggccaacggcgagttccactctggcgagcagctgggcgagacactg
ggcatgtcccgtgccgccatcaacaagcacatccagaccctgcgtgactggggcgtggacgtgttcaccgtgcctggca
agggctacagcctgcccgagcctatccagctgctgaacgccaagcagatcctgggccagctggatggcggatctgtggc
tgtgctgcccgtgatcgacagcaccaaccagtacctgctggaccggatcggcgagctgaaaagcggcgacgcctgtatc
gccgagtatcagcaggctggccgtggccgtcggggccgtaagtggtttagccccttcggcgcaaatctgtacctgagca
tgttctggcggctggaacagggacctgccgccgctattggactgagcctcgtgatcggcatcgtgatggccgaggtgct
gcgtaagctgggtgccgacaaagtgcgcgtgaagtggcccaacgacctgtatctgcaagaccgtaagctggcaggcatc
ctggtggaactgacaggcaaaactggcgacgccgcccagatcgtgattggcgctggcatcaatatggccatgcgtcggg
tggaagagtccgtcgtgaatcagggctggatcaccctgcaagaagccggtatcaacctggaccgtaacaccctggccgc
catgctgatccgtgagctgcgtgccgccctggaactgttcgagcaggaaggcctggccccctacctgtcccgttgggag
aagctggacaacttcatcaaccgtcccgtgaaactgatcatcggcgacaaagagatcttcggcatcagccggggcatcg
acaagcagggcgctctgctgctggaacaggacggcatcatcaagccctggatgggcggcgagatcagcctgcgttctgc
cgagaaaggtgcctggtcccacccccagttcgagaaggatgagctgtaatactagagacgggtggcatccctgtgaccc
ctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatc
attttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaaga
caacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgc
ctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaa
tttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacc
caccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttt