LOCUS       BBa_K2170001            2526 bp    DNA     linear       26-Nov-2024
DEFINITION  Secretory eukaryotic biotin binding receptor with enhanced 
            monomeric avidin
ACCESSION   BBa_K2170001
VERSION     BBa_K2170001.1  
FEATURES             Location/Qualifiers
     promoter        1..559
                     /label=CMV
     promoter        604..622
                     /label=T7
     misc_feature    632..643
                     /label=extended Kozak sequence
     CDS             641..691
                     /label=BM40 signal peptide
     CDS             701..1084
                     /label=eMA
     misc_feature    1091..1123
                     /label=A3C5 Tag
     CDS             1130..1159
                     /label=Poly G linker
     CDS             1166..1234
                     /label=EGFR TMD
     CDS             1235..1261
                     /label=Stop transfer sequence
     CDS             1262..1291
                     /label=poly G linker
     CDS             1298..2005
                     /label=mRuby3
     misc_feature    2012..2035
                     /label=Strep TagII
     polyA_site      2046..2526
                     /label=polyA
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagaact cactataggg
      601 acttaatacg actcactata gggactagac cgccgccacc atgcgggcct ggatcttctt
      661 cctgctgtgc ctggctggca gagccctggc tgccaccggc tttgatgcca gcaacttcaa
      721 agactttagc agcattgcaa gcgcaagcag cagctggcag aatcagcatg gtagcaccat
      781 gattattcag gttgatagct ttggtaatgt gagcggtcag tatgttaatc gtgcagaagg
      841 caccggctgt cagaatagcc cgtatccgct gaccggccgt gttaatggca cctttattga
      901 ttttagcgtg aagtggaata acagcaccga aaattgtaat agcaataccc agtggaccgg
      961 ctatgcacag gttaatggta ataataccga aattgtgacc cgctggaacc tgaaatatga
     1021 aggtggtagc ggtccggcaa tttggcaggg tcaagatacc tttcagtatg tgccgaccac
     1081 cgaaaccggc ttcgacaaaa actacgtagc taactctggt aagaccggcg gaggcggagg
     1141 atctggcgga ggcggatctc cttctatcgc caccggaatg gtgggagccc tcctgctgct
     1201 gctggtggtg gctctgggca tcggcctgtt catgcggcgg agacacatcg tgcggaagag
     1261 aggcggcgga ggaagcggag ggggcggatc taccggcgtg agcaaaggtg aagaactgat
     1321 caaagaaaac atgcggatga aagttgttat ggaaggtagc gttaatggcc accagtttaa
     1381 atgtactggt gaaggtgaag gtcgtccgta tgaaggtgtt caggttatgc gtattaaagt
     1441 gattgaaggt ggtccgctgc cgtttgcatt tgatattctg gcaaccagct ttatgtatgg
     1501 tagccgtacc tttatcaaat atccggcaga tattccggac ttctttaaac agagctttcc
     1561 ggaaggtttt acctgggaac gtgttacccg ttatgaagat ggtggtgttg ttaccgttac
     1621 ccaggatacc agcctggaag atggcgaact ggtttataat gttaaagtgc gtggtgtgaa
     1681 ttttccgagc aatggtccgg ttatgcagaa aaaaaccaaa ggttgggaac cgaataccga
     1741 aatgatgtat cctgccgatg gtggtctgcg tggttatacc gatattgcac tgaaagttga
     1801 tggtggcggt catctgcatt gtaattttgt taccacctac cgctcaaaaa aaaccgtggg
     1861 taacattaaa atgcctggtg ttcatgcagt tgatcatcgt ctggaacgta ttgaagaaag
     1921 cgataatgaa acctatgttg tgcagcgtga agttgcagtt gcaaaatata gcaatctggg
     1981 tggtggtatg gatgagctgt ataaaaccgg ctggtcccac ccccagttcg agaagtaata
     2041 ctagagacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     2101 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     2161 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     2221 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     2281 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     2341 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     2401 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     2461 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     2521 tccttt
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