LOCUS       BBa_K2170002            3813 bp    DNA     linear       23-Nov-2024
DEFINITION  Secretory eukaryotic biotin binding receptor with single chain 
            avidin
ACCESSION   BBa_K2170002
VERSION     BBa_K2170002.1  
FEATURES             Location/Qualifiers
     promoter        1..559
                     /label=CMV
     promoter        604..622
                     /label=T7
     misc_feature    632..643
                     /label=extended Kozak sequence
     CDS             641..691
                     /label=BM40 signal peptide
     CDS             701..2371
                     /label=scAvidin
     misc_feature    2378..2410
                     /label=A3C5 Tag
     CDS             2417..2446
                     /label=poly G linker
     CDS             2453..2521
                     /label=EGFR TMD
     CDS             2522..2548
                     /label=EGFR stop transfer sequence
     CDS             2549..2578
                     /label=poly G linker
     CDS             2585..3292
                     /label=mRuby3
     misc_feature    3299..3322
                     /label=Strep TagII
     polyA_site      3333..3813
                     /label=polyA
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagaact cactataggg
      601 acttaatacg actcactata gggactagac cgccgccacc atgcgggcct ggatcttctt
      661 cctgctgtgc ctggctggca gagccctggc tgccaccggc gctagaaaaa gaacccagcc
      721 taccttcggc ttcaccgtga actggaagtt cagcgagagc accaccgtgt tcaccggcca
      781 gtgcttcatc gacagaaacg gcaaagaggt gctgaaaacc atgtggctgc tgagaagcag
      841 cgtgaacgac atcggcgacg actggaaggc caccagagtg ggcatcaaca tcttcaccag
      901 actgaggacc cagaaagagg gcggctctgg cggaagcgcc agaaagtgta gcctgaccgg
      961 caagtggacc aacgacctgg gcagcaacat gaccatcggc gccgtgaaca gcagaggcga
     1021 gttcacaggc acctacatca ccgccgtgac cgccaccagc aacgagatca aagagagccc
     1081 cctgcacggc acccagaaca ccatcaacaa gagcggcggc agcacaacag tgtttacagg
     1141 acagtgtttt atcgaccgga atgggaaaga agtgctgaaa acaatgtggc tgctgcggtc
     1201 ctccgtgaac gacattggag atgattggaa agctacacga gtggggatta acatttttac
     1261 ccggctgcgc acacagaaag aagggggcag cggcggctcc gctagaaagt gttctctgac
     1321 tggaaaatgg acaaacgatc tggggtccaa tatgacaatc ggggcagtga actctagggg
     1381 cgagtttacc ggaacatata ttacagccgt gacagctacc tctaacgaaa tcaaagagtc
     1441 tcctctgcac gggacacaga ataccattaa caaaagaacc cagcccacat tcgggtttac
     1501 agtgaattgg aaattctccg agggcggcag cggaagcgga tctggctctg gatctggcag
     1561 gacacagccc acctttggat tcactgtgaa ttggaagttt tctgagtcta ccacagtgtt
     1621 cactgggcag tgtttcattg atcgcaatgg aaaagaggtg ctgaaaacta tgtggctgct
     1681 gcgctcaagt gtgaatgaca tcggggatga ttggaaggca actcgcgtgg gaatcaatat
     1741 ctttacacgg ctgagaactc agaaagaggg gggaagcgga ggcagcgccc ggaaatgctc
     1801 tctgacaggg aagtggacta atgatctggg ctctaacatg actattggag ctgtgaatag
     1861 ccggggagag ttcaccggga cttatatcac tgctgtgact gccacctcaa atgagatcaa
     1921 agaatccccc ctgcatggaa cacagaacac tattaacaag tccggcggct ccacaaccgt
     1981 gttcacaggg cagtgcttta ttgaccggaa cggcaaagag gtgctgaaaa caatgtggct
     2041 gctgcgaagc tctgtgaatg atattgggga cgactggaaa gcaactagag tggggatcaa
     2101 tattttcact cgcctgcgga cccagaaaga aggcggaagc ggaggatctg ccagaaagtg
     2161 ctcactgaca ggcaaatgga caaatgacct ggggagtaat atgactattg gggccgtgaa
     2221 cagtcgcggc gagtttactg ggacttacat taccgcagtg acagcaacat ccaatgagat
     2281 caaagaaagt cctctgcatg gcactcagaa cacaatcaac aaaaggaccc agccaacctt
     2341 tggctttacc gtgaattgga agttctctga aaccggcttc gacaaaaact acgtagctaa
     2401 ctctggtaag accggcggag gcggaggatc tggcggaggc ggatctcctt ctatcgccac
     2461 cggaatggtg ggagccctcc tgctgctgct ggtggtggct ctgggcatcg gcctgttcat
     2521 gcggcggaga cacatcgtgc ggaagagagg cggcggagga agcggagggg gcggatctac
     2581 cggcgtgagc aaaggtgaag aactgatcaa agaaaacatg cggatgaaag ttgttatgga
     2641 aggtagcgtt aatggccacc agtttaaatg tactggtgaa ggtgaaggtc gtccgtatga
     2701 aggtgttcag gttatgcgta ttaaagtgat tgaaggtggt ccgctgccgt ttgcatttga
     2761 tattctggca accagcttta tgtatggtag ccgtaccttt atcaaatatc cggcagatat
     2821 tccggacttc tttaaacaga gctttccgga aggttttacc tgggaacgtg ttacccgtta
     2881 tgaagatggt ggtgttgtta ccgttaccca ggataccagc ctggaagatg gcgaactggt
     2941 ttataatgtt aaagtgcgtg gtgtgaattt tccgagcaat ggtccggtta tgcagaaaaa
     3001 aaccaaaggt tgggaaccga ataccgaaat gatgtatcct gccgatggtg gtctgcgtgg
     3061 ttataccgat attgcactga aagttgatgg tggcggtcat ctgcattgta attttgttac
     3121 cacctaccgc tcaaaaaaaa ccgtgggtaa cattaaaatg cctggtgttc atgcagttga
     3181 tcatcgtctg gaacgtattg aagaaagcga taatgaaacc tatgttgtgc agcgtgaagt
     3241 tgcagttgca aaatatagca atctgggtgg tggtatggat gagctgtata aaaccggctg
     3301 gtcccacccc cagttcgaga agtaatacta gagacgggtg gcatccctgt gacccctccc
     3361 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     3421 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     3481 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     3541 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     3601 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     3661 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     3721 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     3781 aggcgtgaac cactgctccc ttccctgtcc ttt
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