##gff-version 3
##sequence-region BBa_K2170002 1 3813
BBa_K2170002	.	promoter	1	559	.	+	0	ID=annotation2513388;Name=CMV
BBa_K2170002	.	sequence_feature	632	643	.	+	0	ID=annotation2513390;Name=extended Kozak sequence
BBa_K2170002	.	polyA_site	3333	3813	.	+	0	ID=annotation2513400;Name=polyA
BBa_K2170002	.	tag	2378	2410	.	+	0	ID=annotation2513393;Name=A3C5 Tag
BBa_K2170002	.	CDS	701	2371	.	+	0	ID=annotation2513392;Name=scAvidin
BBa_K2170002	.	CDS	2522	2548	.	+	0	ID=annotation2513396;Name=EGFR stop transfer sequence
BBa_K2170002	.	CDS	2585	3292	.	+	0	ID=annotation2513398;Name=mRuby3
BBa_K2170002	.	CDS	2417	2446	.	+	0	ID=annotation2513394;Name=poly G linker
BBa_K2170002	.	CDS	2453	2521	.	+	0	ID=annotation2513395;Name=EGFR TMD
BBa_K2170002	.	tag	3299	3322	.	+	0	ID=annotation2513399;Name=Strep TagII
BBa_K2170002	.	CDS	641	691	.	+	0	ID=annotation2513391;Name=BM40 signal peptide
BBa_K2170002	.	promoter	604	622	.	+	0	ID=annotation2513389;Name=T7
BBa_K2170002	.	CDS	2549	2578	.	+	0	ID=annotation2513397;Name=poly G linker
>BBa_K2170002
atagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccg
cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactt
tccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtac
gccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctact
tggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagc
ggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacggga
ctttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagc
agagctggtttagtgaaccgtcagatctactagaactcactatagggacttaatacgactcactatagggactagaccg
ccgccaccatgcgggcctggatcttcttcctgctgtgcctggctggcagagccctggctgccaccggcgctagaaaaag
aacccagcctaccttcggcttcaccgtgaactggaagttcagcgagagcaccaccgtgttcaccggccagtgcttcatc
gacagaaacggcaaagaggtgctgaaaaccatgtggctgctgagaagcagcgtgaacgacatcggcgacgactggaagg
ccaccagagtgggcatcaacatcttcaccagactgaggacccagaaagagggcggctctggcggaagcgccagaaagtg
tagcctgaccggcaagtggaccaacgacctgggcagcaacatgaccatcggcgccgtgaacagcagaggcgagttcaca
ggcacctacatcaccgccgtgaccgccaccagcaacgagatcaaagagagccccctgcacggcacccagaacaccatca
acaagagcggcggcagcacaacagtgtttacaggacagtgttttatcgaccggaatgggaaagaagtgctgaaaacaat
gtggctgctgcggtcctccgtgaacgacattggagatgattggaaagctacacgagtggggattaacatttttacccgg
ctgcgcacacagaaagaagggggcagcggcggctccgctagaaagtgttctctgactggaaaatggacaaacgatctgg
ggtccaatatgacaatcggggcagtgaactctaggggcgagtttaccggaacatatattacagccgtgacagctacctc
taacgaaatcaaagagtctcctctgcacgggacacagaataccattaacaaaagaacccagcccacattcgggtttaca
gtgaattggaaattctccgagggcggcagcggaagcggatctggctctggatctggcaggacacagcccacctttggat
tcactgtgaattggaagttttctgagtctaccacagtgttcactgggcagtgtttcattgatcgcaatggaaaagaggt
gctgaaaactatgtggctgctgcgctcaagtgtgaatgacatcggggatgattggaaggcaactcgcgtgggaatcaat
atctttacacggctgagaactcagaaagaggggggaagcggaggcagcgcccggaaatgctctctgacagggaagtgga
ctaatgatctgggctctaacatgactattggagctgtgaatagccggggagagttcaccgggacttatatcactgctgt
gactgccacctcaaatgagatcaaagaatcccccctgcatggaacacagaacactattaacaagtccggcggctccaca
accgtgttcacagggcagtgctttattgaccggaacggcaaagaggtgctgaaaacaatgtggctgctgcgaagctctg
tgaatgatattggggacgactggaaagcaactagagtggggatcaatattttcactcgcctgcggacccagaaagaagg
cggaagcggaggatctgccagaaagtgctcactgacaggcaaatggacaaatgacctggggagtaatatgactattggg
gccgtgaacagtcgcggcgagtttactgggacttacattaccgcagtgacagcaacatccaatgagatcaaagaaagtc
ctctgcatggcactcagaacacaatcaacaaaaggacccagccaacctttggctttaccgtgaattggaagttctctga
aaccggcttcgacaaaaactacgtagctaactctggtaagaccggcggaggcggaggatctggcggaggcggatctcct
tctatcgccaccggaatggtgggagccctcctgctgctgctggtggtggctctgggcatcggcctgttcatgcggcgga
gacacatcgtgcggaagagaggcggcggaggaagcggagggggcggatctaccggcgtgagcaaaggtgaagaactgat
caaagaaaacatgcggatgaaagttgttatggaaggtagcgttaatggccaccagtttaaatgtactggtgaaggtgaa
ggtcgtccgtatgaaggtgttcaggttatgcgtattaaagtgattgaaggtggtccgctgccgtttgcatttgatattc
tggcaaccagctttatgtatggtagccgtacctttatcaaatatccggcagatattccggacttctttaaacagagctt
tccggaaggttttacctgggaacgtgttacccgttatgaagatggtggtgttgttaccgttacccaggataccagcctg
gaagatggcgaactggtttataatgttaaagtgcgtggtgtgaattttccgagcaatggtccggttatgcagaaaaaaa
ccaaaggttgggaaccgaataccgaaatgatgtatcctgccgatggtggtctgcgtggttataccgatattgcactgaa
agttgatggtggcggtcatctgcattgtaattttgttaccacctaccgctcaaaaaaaaccgtgggtaacattaaaatg
cctggtgttcatgcagttgatcatcgtctggaacgtattgaagaaagcgataatgaaacctatgttgtgcagcgtgaag
ttgcagttgcaaaatatagcaatctgggtggtggtatggatgagctgtataaaaccggctggtcccacccccagttcga
gaagtaatactagagacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagt
gcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtgg
aggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctgga
gtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagtt
gttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggcc
aggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaacca
ctgctcccttccctgtccttt